Detailed information of regulator
|Regulatory pathway (from literatures)||QS regulatory pathway|
|KEGG pathway (determined by BlastKOALA)||Quorum sensing (map02024); Biofilm formation - Pseudomonas aeruginosa (map02025)|
|Related T6SS||T6SS00152 (Type i1); T6SS00151 (Type i4a); T6SS00150 (Type i3)|
|Strain||Pseudomonas aeruginosa UCBPP-PA14|
|Sequence||Protein sequence (240 a.a.); Nucleotide sequence (720 bp)|
|Description||Transcription of hcp2 and hcp3 was affected in the QS mutants, demonstrating that LasR control hcp2 and hcp3 expression. In contrast, hcp1 transcript levels were maintained at low levels of expression, which increased in ΔlasR mutant, suggesting that LasR repress the HSI-I effector hcp1.; No LasR binding sites in either HSI-I or HSI-III were found, suggesting that LasR does not directly bind to these loci.Transcription of hcp2 and hcp3 was affected in the QS mutants ΔlasR, demonstrating that LasR control hcp2 and hcp3 expression. In contrast, hcp1 transcript levels were maintained at low levels of expression, which increased in ΔlasR mutant, suggesting that LasR repress the HSI-I effector hcp1.|
 Lesic B, Starkey M, He J, et al. Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis. Microbiology (Reading). 2009 Sep;155(Pt 9):2845-2855. doi: 10.1099/mic.0.029082-0. Epub 2009 Jun 4. PMID: 19497948
External database links
|Locus tag (Gene)||PA14_45960 (lasR)|
|Coordinates (Strand)||4085339..4086058 (-)|
|Uniprot ID||V6AHU1_PSEAI, A0A1F0I3V8_9PSED, A0A0H2Z901_PSEAB, LASR_PSEAE, A0A0C7AE60_PSEAI|
|KEGG ID||pau:PA14_45960, pae:PA1430|
|KEGG pathway (determined by BlastKOALA)||map02024; map02025|
|PDB ID||3IX4, 6D6A, 6V7W, 6D6O, 6MWL, 6MVM, 2UV0, 3JPU, 6D6D, 6D6L, 6D6M, 6MWW, 4NG2, 6MWH, 6D6C, 6V7X, 6MVN, 6D6P, 3IX8, 3IX3, 6D6N, 6MWZ, 6D6B|
|Target||Regulation (↓/↑)||Target sequence|
|TssA2 (PA14_43050) promotor||↓||A LasR box (AACTACCTGTTTTGGTAGGG)|
Pfam domain hit(s) of regulator
|Domain||Pfam ID||E-value||Aligned region|
- Transmembrane helices are predicted using TMHMM 2.0 software.
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
|Prediction||Probability||Cleavage site||Signal peptide sequence|
Protein sequence of regulator: 240 a.a. .
Nucleotide sequence of regulator: 720 bp .