Detailed information of regulator


Summary

Regulator ID REG0166 experimental
Regulator type protein
Regulatory pathway (from literatures) QS regulatory pathway
KEGG pathway (determined by BlastKOALA) Quorum sensing (map02024); Biofilm formation - Pseudomonas aeruginosa (map02025)
Related T6SS T6SS00152 experimental (Type i1); T6SS00151 experimental (Type i4a); T6SS00150 experimental (Type i3)
Strain Pseudomonas aeruginosa UCBPP-PA14
Replicon chromosome
Sequence Protein sequence (240 a.a.); Nucleotide sequence (720 bp)
Description Transcription of hcp2 and hcp3 was affected in the QS mutants, demonstrating that LasR control hcp2 and hcp3 expression. In contrast, hcp1 transcript levels were maintained at low levels of expression, which increased in ΔlasR mutant, suggesting that LasR repress the HSI-I effector hcp1.; No LasR binding sites in either HSI-I or HSI-III were found, suggesting that LasR does not directly bind to these loci.Transcription of hcp2 and hcp3 was affected in the QS mutants ΔlasR, demonstrating that LasR control hcp2 and hcp3 expression. In contrast, hcp1 transcript levels were maintained at low levels of expression, which increased in ΔlasR mutant, suggesting that LasR repress the HSI-I effector hcp1.
Reference

External database links

Locus tag (Gene) PA14_45960 (lasR)
Coordinates (Strand) 4085339..4086058 (-)
NCBI ID 489173455
RefSeq NC_008463
Uniprot ID V6AHU1_PSEAI, A0A0H2Z901_PSEAB, LASR_PSEAE, A0A0C7AE60_PSEAI
KEGG ID pau:PA14_45960, pae:PA1430
KEGG pathway (determined by BlastKOALA) map02024; map02025
PDB ID 3IX4, 6D6A, 6V7W, 6D6O, 6MWL, 6MVM, 2UV0, 3JPU, 6D6D, 6D6L, 6D6M, 6MWW, 4NG2, 6MWH, 6D6C, 6V7X, 6MVN, 6D6P, 3IX8, 3IX3, 6D6N, 6MWZ, 6D6B

  Target
Target Regulation (↓/↑) Target sequence
TssA2 (PA14_43050) promotor A LasR box (AACTACCTGTTTTGGTAGGG)
HSI-III transcripts -
HSI-I transcripts -

Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Autoind_bind PF03472.17 1.3e-37 18..160
GerE PF00196.21 3.1e-22 176..231

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-239 MALVDGFLELERSSGKLEWSAILQKMASDLGFSKILFGLLPKDSQDYENAFIVGNYPAAWREHYDRAGYARVDPTVSHCTQSVLPIFWEPSIYQTRKQHEFFEEASAAGLVYGLTMPLHGARGELGALSLSVEAENRAEANRFMESVLPTLWMLKDYALQSGAGLAFEHPVSKPVVLTSREKEVLQWCAIGKTSWEISVICNCSEANVNFHMGNIRRKFGVTSRRVAAIMAVNLGLITL



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9926 - -



  Protein sequence of regulator: 240 a.a.    .

>REG0166 NC_008463:c4086058-4085339 [Pseudomonas aeruginosa UCBPP-PA14]
MALVDGFLELERSSGKLEWSAILQKMASDLGFSKILFGLLPKDSQDYENAFIVGNYPAAWREHYDRAGYARVDPTVSHCT
QSVLPIFWEPSIYQTRKQHEFFEEASAAGLVYGLTMPLHGARGELGALSLSVEAENRAEANRFMESVLPTLWMLKDYALQ
SGAGLAFEHPVSKPVVLTSREKEVLQWCAIGKTSWEISVICNCSEANVNFHMGNIRRKFGVTSRRVAAIMAVNLGLITL*

  Nucleotide sequence of regulator: 720 bp    .

>REG0166 NC_008463:c4086058-4085339 [Pseudomonas aeruginosa UCBPP-PA14]
ATGGCCTTGGTTGACGGTTTTCTTGAGCTGGAACGCTCAAGTGGAAAATTGGAGTGGAGCGCCATCCTGCAGAAGATGGC
GAGCGACCTTGGATTCTCGAAGATCCTCTTCGGCCTGTTGCCTAAGGACAGCCAGGACTACGAGAACGCCTTCATCGTCG
GCAACTACCCGGCCGCCTGGCGCGAGCATTACGACCGGGCTGGCTACGCGCGGGTCGACCCAACGGTCAGTCACTGTACC
CAGAGCGTACTGCCGATTTTCTGGGAACCGTCCATCTACCAGACGCGAAAGCAGCACGAGTTCTTCGAGGAAGCCTCGGC
GGCCGGCCTGGTGTATGGGCTGACCATGCCGCTGCATGGTGCGCGCGGCGAACTCGGCGCGCTGAGCCTCAGCGTGGAAG
CGGAAAACCGGGCCGAGGCCAACCGTTTCATGGAGTCGGTCCTGCCGACCCTGTGGATGCTCAAGGACTACGCACTGCAG
AGCGGTGCCGGACTGGCCTTCGAACATCCGGTCAGCAAACCGGTGGTTCTGACCAGCCGGGAGAAGGAAGTGTTGCAGTG
GTGCGCCATCGGCAAGACCAGTTGGGAGATATCGGTTATCTGCAACTGCTCGGAAGCCAATGTGAACTTCCATATGGGAA
ATATTCGGCGGAAGTTCGGTGTGACCTCCCGCCGCGTAGCGGCCATTATGGCCGTTAATTTGGGTCTTATTACTCTCTGA