Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00151 experimental
T6SS type Type i4a
Strain Pseudomonas aeruginosa UCBPP-PA14
Replicon (RefSeq) chromosome (NC_008463)
Location 3011563..3044708
Function anti-eukaryotic; virulence
Comment Needed for full virulence in mouse lung and acute burn model; required for wild-type infection of Arabidopsis.
References
[1] Lesic B et al (2009) Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis. Microbiology. 155(Pt 9):2845-55. [PudMed:19497948]
[2] Barret M et al (2011) Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentSfa3TssATssBTssCTssDTssETssFTssGTssHTssITssKTssLTssM
Number1111111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
PA14_RS13795 3007457..3007780 + 324 WP_003089535.1 transcriptional repressor
PA14_RS13800 3007822..3008742 + 921 WP_003124571.1 LysR family transcriptional regulator
PA14_RS13805 3008839..3009990 + 1152 WP_003104946.1 L-lactate dehydrogenase LldA
PA14_RS13810 3010057..3010299 - 243 WP_003089526.1 DUF1654 domain-containing protein
PA14_RS13815 3010594..3010830 + 237 WP_003104944.1 hypothetical protein
PA14_RS13820 3011096..3011566 + 471 WP_003089521.1 (2Fe-2S)-binding protein
PA14_RS13825 3011563..3013878 + 2316 WP_003139301.1 xanthine dehydrogenase family protein molybdopterin-binding subunit
PA14_RS13830 3014295..3015500 - 1206 WP_003089517.1 ABC transporter substrate-binding protein
PA14_RS13835 3015701..3016342 - 642 WP_003139305.1 response regulator transcription factor
PA14_RS13840 3016619..3017014 + 396 WP_003089513.1 DUF4280 domain-containing protein
PA14_RS13845 3017037..3017573 - 537 WP_003139307.1 toxin-antitoxin system YwqK family antitoxin
T6CP001468 TssI PA14_RS13850 (tssI) 3017584..3019590 - 2007 WP_003139309.1 type VI secretion system tip protein VgrG
PA14_RS13855 3019627..3020694 - 1068 WP_003124577.1 hypothetical protein
PA14_RS13860 3020733..3021266 - 534 WP_003089506.1 hypothetical protein
T6CP001469 TssH PA14_RS13865 (tssH) 3021340..3023889 - 2550 WP_003139311.1 type VI secretion system ATPase TssH
T6CP001470 TssG PA14_RS13870 (tssG) 3023891..3024907 - 1017 WP_003139313.1 type VI secretion system baseplate subunit TssG
T6CP001471 TssF PA14_RS13875 (tssF) 3024871..3026664 - 1794 WP_003089497.1 type VI secretion system baseplate subunit TssF
T6CP009589 TssE PA14_RS13880 (tssE) 3026648..3027073 - 426 WP_003089496.1 type VI secretion system baseplate subunit TssE
T6CP001472 TssD PA14_RS13885 3027086..3027583 - 498 WP_003089495.1 type VI secretion system tube protein Hcp
T6CP001473 TssC PA14_RS13890 (tssC) 3027657..3029141 - 1485 WP_003139317.1 type VI secretion system contractile sheath large subunit
T6CP001474 TssB PA14_RS13895 (tssB) 3029164..3029709 - 546 WP_003089493.1 type VI secretion system contractile sheath small subunit
PA14_RS13900 3029917..3030393 + 477 WP_003089492.1 hypothetical protein
T6CP001475 TssK PA14_RS13905 (tssK) 3030453..3031784 + 1332 WP_003139318.1 type VI secretion system baseplate subunit TssK
T6CP009588 TssL PA14_RS13910 3031802..3032560 + 759 WP_003131567.1 DotU family type IV/VI secretion system protein
T6CP001476 TssM PA14_RS13915 3032557..3036372 + 3816 WP_003139320.1 type VI secretion system membrane subunit
T6CP001477 TssA PA14_RS13920 (tssA) 3036369..3037469 + 1101 WP_003139323.1 type VI secretion system protein TssA
T6CP012460 Sfa3 PA14_RS13925 3037579..3038664 + 1086 WP_003139325.1 sigma-54-dependent transcriptional regulator
PA14_RS13930 3038900..3039166 + 267 WP_003104712.1 hypothetical protein
PA14_RS13935 (msuE) 3039326..3039886 + 561 WP_003089470.1 FMN reductase
PA14_RS13940 (ssuD) 3039896..3041041 + 1146 WP_003104713.1 FMNH2-dependent alkanesulfonate monooxygenase
PA14_RS13945 3041071..3042255 + 1185 WP_003139331.1 acyl-CoA dehydrogenase family protein
PA14_RS13950 3042252..3043382 + 1131 WP_003139334.1 sigma-54 dependent transcriptional regulator
PA14_RS13955 3043554..3044708 + 1155 WP_003139336.1 cell division protein ZapE
PA14_RS13960 3044774..3045901 - 1128 WP_003139338.1 glycerophosphodiester phosphodiesterase
PA14_RS13965 3046079..3046732 - 654 WP_003111646.1 ABC transporter permease
PA14_RS13970 3046716..3047825 - 1110 WP_003139341.1 methionine ABC transporter ATP-binding protein
PA14_RS13975 3047822..3048616 - 795 WP_003139343.1 MetQ/NlpA family ABC transporter substrate-binding protein
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0166 experimental PA14_45960 (lasR) protein QS regulatory pathway Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 4085339..4086058 (-) 489173455 TssA2 (PA14_43050) promotor (↓); HSI-III transcripts (↓); HSI-I transcripts (↑)
REG0167 experimental PA14_51340 (MvfR) protein QS regulatory pathway Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 4562837..4563835 (+) 489176730 Upstream of pqs operon (↓); HSI-III transcripts (↓); HSI-I transcripts (↑)
REG0169 experimental PA14_22470 (PA3225) protein Global regulator Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 1955624..1956553 (-) 489182081 PA14_00990 (TssA) promotor (↓); TssA1 (PA14_00990) transcript (↑); TssA2 (PA14_43050) transcript (↑); TssB3 (PA14_34070) transcript (↑)
REG0170 experimental PA14_20290 (AmrZ) protein Global regulator Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 1747602..1747928 (-) 489182353 TssA1 (PA14_00990) promotor (↓); TssA2 (PA14_43050) promotor (↑); TssB3 (PA14_34070) promotor (↓)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF01817 experimental PA14_03220 (VgrG2b) Tpe - Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 285213..288272 (-) 489228784
EFF01822 experimental PA14_34030 (hcp3) - - Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 3027086..3027583 (-) 489180008

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector