Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00151  |
| T6SS type | Type i4a |
| Strain | Pseudomonas aeruginosa UCBPP-PA14 |
| Replicon (RefSeq) | chromosome (NC_008463) |
| Location | 3011563..3044708 |
| Function | anti-eukaryotic; virulence |
| Comment | Needed for full virulence in mouse lung and acute burn model; required for wild-type infection of Arabidopsis. |
| References | [1] Lesic B et al (2009) Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis. Microbiology. 155(Pt 9):2845-55. [PudMed:19497948] [2] Barret M et al (2011) Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | Sfa3 | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssK | TssL | TssM |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| PA14_RS13795 | 3007457..3007780 | + | 324 | WP_003089535.1 | transcriptional repressor | ||
| PA14_RS13800 | 3007822..3008742 | + | 921 | WP_003124571.1 | LysR family transcriptional regulator | ||
| PA14_RS13805 | 3008839..3009990 | + | 1152 | WP_003104946.1 | L-lactate dehydrogenase LldA | ||
| PA14_RS13810 | 3010057..3010299 | - | 243 | WP_003089526.1 | DUF1654 domain-containing protein | ||
| PA14_RS13815 | 3010594..3010830 | + | 237 | WP_003104944.1 | hypothetical protein | ||
| PA14_RS13820 | 3011096..3011566 | + | 471 | WP_003089521.1 | (2Fe-2S)-binding protein | ||
| PA14_RS13825 | 3011563..3013878 | + | 2316 | WP_003139301.1 | xanthine dehydrogenase family protein molybdopterin-binding subunit | ||
| PA14_RS13830 | 3014295..3015500 | - | 1206 | WP_003089517.1 | ABC transporter substrate-binding protein | ||
| PA14_RS13835 | 3015701..3016342 | - | 642 | WP_003139305.1 | response regulator transcription factor | ||
| PA14_RS13840 | 3016619..3017014 | + | 396 | WP_003089513.1 | DUF4280 domain-containing protein | ||
| PA14_RS13845 | 3017037..3017573 | - | 537 | WP_003139307.1 | toxin-antitoxin system YwqK family antitoxin | ||
| T6CP001468 | TssI | PA14_RS13850 (tssI) | 3017584..3019590 | - | 2007 | WP_003139309.1 | type VI secretion system tip protein VgrG |
| PA14_RS13855 | 3019627..3020694 | - | 1068 | WP_003124577.1 | hypothetical protein | ||
| PA14_RS13860 | 3020733..3021266 | - | 534 | WP_003089506.1 | hypothetical protein | ||
| T6CP001469 | TssH | PA14_RS13865 (tssH) | 3021340..3023889 | - | 2550 | WP_003139311.1 | type VI secretion system ATPase TssH |
| T6CP001470 | TssG | PA14_RS13870 (tssG) | 3023891..3024907 | - | 1017 | WP_003139313.1 | type VI secretion system baseplate subunit TssG |
| T6CP001471 | TssF | PA14_RS13875 (tssF) | 3024871..3026664 | - | 1794 | WP_003089497.1 | type VI secretion system baseplate subunit TssF |
| T6CP009589 | TssE | PA14_RS13880 (tssE) | 3026648..3027073 | - | 426 | WP_003089496.1 | type VI secretion system baseplate subunit TssE |
| T6CP001472 | TssD | PA14_RS13885 | 3027086..3027583 | - | 498 | WP_003089495.1 | type VI secretion system tube protein Hcp |
| T6CP001473 | TssC | PA14_RS13890 (tssC) | 3027657..3029141 | - | 1485 | WP_003139317.1 | type VI secretion system contractile sheath large subunit |
| T6CP001474 | TssB | PA14_RS13895 (tssB) | 3029164..3029709 | - | 546 | WP_003089493.1 | type VI secretion system contractile sheath small subunit |
| PA14_RS13900 | 3029917..3030393 | + | 477 | WP_003089492.1 | hypothetical protein | ||
| T6CP001475 | TssK | PA14_RS13905 (tssK) | 3030453..3031784 | + | 1332 | WP_003139318.1 | type VI secretion system baseplate subunit TssK |
| T6CP009588 | TssL | PA14_RS13910 | 3031802..3032560 | + | 759 | WP_003131567.1 | DotU family type IV/VI secretion system protein |
| T6CP001476 | TssM | PA14_RS13915 | 3032557..3036372 | + | 3816 | WP_003139320.1 | type VI secretion system membrane subunit |
| T6CP001477 | TssA | PA14_RS13920 (tssA) | 3036369..3037469 | + | 1101 | WP_003139323.1 | type VI secretion system protein TssA |
| T6CP012460 | Sfa3 | PA14_RS13925 | 3037579..3038664 | + | 1086 | WP_003139325.1 | sigma-54-dependent transcriptional regulator |
| PA14_RS13930 | 3038900..3039166 | + | 267 | WP_003104712.1 | hypothetical protein | ||
| PA14_RS13935 (msuE) | 3039326..3039886 | + | 561 | WP_003089470.1 | FMN reductase | ||
| PA14_RS13940 (ssuD) | 3039896..3041041 | + | 1146 | WP_003104713.1 | FMNH2-dependent alkanesulfonate monooxygenase | ||
| PA14_RS13945 | 3041071..3042255 | + | 1185 | WP_003139331.1 | acyl-CoA dehydrogenase family protein | ||
| PA14_RS13950 | 3042252..3043382 | + | 1131 | WP_003139334.1 | sigma-54 dependent transcriptional regulator | ||
| PA14_RS13955 | 3043554..3044708 | + | 1155 | WP_003139336.1 | cell division protein ZapE | ||
| PA14_RS13960 | 3044774..3045901 | - | 1128 | WP_003139338.1 | glycerophosphodiester phosphodiesterase | ||
| PA14_RS13965 | 3046079..3046732 | - | 654 | WP_003111646.1 | ABC transporter permease | ||
| PA14_RS13970 | 3046716..3047825 | - | 1110 | WP_003139341.1 | methionine ABC transporter ATP-binding protein | ||
| PA14_RS13975 | 3047822..3048616 | - | 795 | WP_003139343.1 | MetQ/NlpA family ABC transporter substrate-binding protein |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|---|---|---|---|---|---|---|---|
| REG0166 |
PA14_45960 (lasR) | protein | QS regulatory pathway | Pseudomonas aeruginosa UCBPP-PA14 | chromosome (NC_008463) | 4085339..4086058 (-) | 489173455 | TssA2 (PA14_43050) promotor (↓); HSI-III transcripts (↓); HSI-I transcripts (↑) |
| REG0167 |
PA14_51340 (MvfR) | protein | QS regulatory pathway | Pseudomonas aeruginosa UCBPP-PA14 | chromosome (NC_008463) | 4562837..4563835 (+) | 489176730 | Upstream of pqs operon (↓); HSI-III transcripts (↓); HSI-I transcripts (↑) |
| REG0169 |
PA14_22470 (PA3225) | protein | Global regulator | Pseudomonas aeruginosa UCBPP-PA14 | chromosome (NC_008463) | 1955624..1956553 (-) | 489182081 | PA14_00990 (TssA) promotor (↓); TssA1 (PA14_00990) transcript (↑); TssA2 (PA14_43050) transcript (↑); TssB3 (PA14_34070) transcript (↑) |
| REG0170 |
PA14_20290 (AmrZ) | protein | Global regulator | Pseudomonas aeruginosa UCBPP-PA14 | chromosome (NC_008463) | 1747602..1747928 (-) | 489182353 | TssA1 (PA14_00990) promotor (↓); TssA2 (PA14_43050) promotor (↑); TssB3 (PA14_34070) promotor (↓) |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF01817 |
PA14_03220 (VgrG2b) | Tpe | - | Pseudomonas aeruginosa UCBPP-PA14 | chromosome (NC_008463) | 285213..288272 (-) | 489228784 |
| EFF01822 |
PA14_34030 (hcp3) | - | - | Pseudomonas aeruginosa UCBPP-PA14 | chromosome (NC_008463) | 3027086..3027583 (-) | 489180008 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
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