SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP009588
Component protein type	TssL
T6SS ID (Type)	T6SS00151 (Type i4a)
Strain	Pseudomonas aeruginosa UCBPP-PA14
Replicon	chromosome
Sequence	Protein sequence (253 a.a.); Nucleotide sequence (759 bp)
Reference	[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537 [2] Lesic B, Starkey M, He J, et al. Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis. Microbiology (Reading). 2009 Sep;155(Pt 9):2845-2855. doi: 10.1099/mic.0.029082-0. Epub 2009 Jun 4. PMID: 19497948

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PA14_RS13910
Coordinate (Strand)	3031802..3032560 (+)
NCBI ID	WP_003131567.1
RefSeq	NC_008463
Uniprot ID	V6AEN5_PSEAI, A0A0H2ZBD5_PSEAB, A0A6N0KLD1_9PSED, A0A077JVX9_PSEAI
KEGG ID	pau:PA14_34110
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	2.1e-29	72..227

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-209	MPEGSAGPFSQAYQEQPLSTAFRQAWQEWLEAWGALDRDAQDVPRMVERALELSTRITRRLWRSAFASVGDAAGVQVKAMVYAFVALVDETLVFSAWPGQGAWQEKPLESHLYGSRQAGEYLPLAIKRLLDERAPASRDLANVYLQCLILGFRGRLRGPRGEALHEKWRQALFAFAWQREADAADLGRRLEQPAAAPPRRLPVRAALPD
Transmembrane helix	210-232	GFRLGLAVLGLVLLMSGIGHLFW
Inside	233-252	RDIQHEMAAVTHLADAEQAP

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9886	-	-

Protein sequence: 253 a.a.

						>T6CP009588 NC_008463:3031802-3032560 [Pseudomonas aeruginosa UCBPP-PA14] [TssL]
MPEGSAGPFSQAYQEQPLSTAFRQAWQEWLEAWGALDRDAQDVPRMVERALELSTRITRRLWRSAFASVGDAAGVQVKAM
VYAFVALVDETLVFSAWPGQGAWQEKPLESHLYGSRQAGEYLPLAIKRLLDERAPASRDLANVYLQCLILGFRGRLRGPR
GEALHEKWRQALFAFAWQREADAADLGRRLEQPAAAPPRRLPVRAALPDGFRLGLAVLGLVLLMSGIGHLFWRDIQHEMA
AVTHLADAEQAP*					

Nucleotide sequence: 759 bp

						>T6CP009588 NC_008463:3031802-3032560 [Pseudomonas aeruginosa UCBPP-PA14] [TssL]
ATGCCCGAAGGGAGTGCAGGGCCTTTTTCCCAGGCGTACCAGGAGCAGCCGCTCAGCACGGCATTCCGCCAGGCCTGGCA
GGAATGGCTGGAGGCCTGGGGCGCCCTCGACCGCGACGCCCAGGATGTGCCACGGATGGTCGAGCGCGCCCTCGAACTGT
CCACCCGCATCACCCGGCGTCTGTGGCGCAGCGCCTTCGCCAGCGTCGGCGACGCGGCGGGCGTGCAGGTCAAGGCGATG
GTCTATGCCTTCGTCGCCCTGGTCGACGAGACCCTGGTCTTCAGCGCCTGGCCGGGGCAGGGCGCCTGGCAGGAAAAACC
GCTGGAGTCGCATCTCTACGGCAGTCGCCAGGCGGGCGAATACCTGCCGCTGGCGATCAAGCGCCTGCTCGACGAACGCG
CCCCGGCCAGTCGCGACCTGGCCAACGTCTACCTGCAATGCCTGATCCTCGGCTTCCGCGGTCGCCTGCGCGGGCCGCGC
GGCGAGGCGCTGCACGAGAAGTGGCGGCAGGCGCTGTTCGCCTTCGCCTGGCAGCGCGAGGCGGACGCCGCCGACCTCGG
CCGTCGCCTTGAGCAGCCTGCCGCCGCGCCGCCACGGCGCCTGCCGGTGCGCGCGGCGCTGCCGGACGGTTTCCGCCTGG
GCCTGGCGGTGCTCGGCCTGGTGCTGCTGATGAGCGGCATCGGGCACCTGTTCTGGCGCGACATCCAGCATGAAATGGCG
GCGGTGACCCACCTGGCCGATGCGGAGCAGGCGCCATGA					