SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001471
Component protein type	TssF
T6SS ID (Type)	T6SS00151 (Type i4a)
Strain	Pseudomonas aeruginosa UCBPP-PA14
Replicon	chromosome
Sequence	Protein sequence (598 a.a.); Nucleotide sequence (1794 bp)
Reference	[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537 [2] Lesic B, Starkey M, He J, et al. Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis. Microbiology (Reading). 2009 Sep;155(Pt 9):2845-2855. doi: 10.1099/mic.0.029082-0. Epub 2009 Jun 4. PMID: 19497948

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PA14_RS13875
Coordinate (Strand)	3024871..3026664 (-)
NCBI ID	WP_003089497.1
RefSeq	NC_008463
Uniprot ID	A0A0H2ZA48_PSEAB, A0A233T148_PSEAI
KEGG ID	pau:PA14_34010
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	4.2e-184	6..593

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-597	MSDSIDADLLDYYQRELTWLRHAGGAFAERYPKVARRLQLAPGECPDPHVERLLEGFSLLTARLQRRLDDDYAEFSDALLEQLYPLALRPLPSCAIVQFEPDPTQGNLAEGYRLPRDTALFVTTRGGDSVHFRSSAEATLWPLAIDEATLLGGEEAQALTGVGAARSALRLSLRCLGEYRWDELSIERLRIHLAASPMGCAILYDLLAAHAVQVMAGAPGTPPAALRGLPEIVGFAADEALLPEEDGGHPGLRLLAEYFAFPDKFHFFDLPLSGALADGQTLYLYLVFDRAPTARLHLQAEDFQLGCAPAINLFPRTSEPLRPDGTRSEYRLVADSHRENSVEIHSIRAVRSVAGSTVQPVPAYYGCRHDSHLGTLYWHARRIAGLTPNRLGSDLLLSLVDTGFDPLREAPAFSLTAELLCTNRYLAERLGAGTALGFERPGPVAAARLRNAPSAQSQPRLDGQSRWRLVSQLTLNHLSLVEGPRALEALREILALHNLRDDASARRQIEGLRQLDCQRVVAHVGEDAWRGWRNGLEIRLRLDPQHFVGASGVLFSAVLAQFFSLYATANRFVRTVLVEADKEVKTWQPQAGSPLSL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9899	-	-

Protein sequence: 598 a.a.

						>T6CP001471 NC_008463:c3026664-3024871 [Pseudomonas aeruginosa UCBPP-PA14] [TssF]
MSDSIDADLLDYYQRELTWLRHAGGAFAERYPKVARRLQLAPGECPDPHVERLLEGFSLLTARLQRRLDDDYAEFSDALL
EQLYPLALRPLPSCAIVQFEPDPTQGNLAEGYRLPRDTALFVTTRGGDSVHFRSSAEATLWPLAIDEATLLGGEEAQALT
GVGAARSALRLSLRCLGEYRWDELSIERLRIHLAASPMGCAILYDLLAAHAVQVMAGAPGTPPAALRGLPEIVGFAADEA
LLPEEDGGHPGLRLLAEYFAFPDKFHFFDLPLSGALADGQTLYLYLVFDRAPTARLHLQAEDFQLGCAPAINLFPRTSEP
LRPDGTRSEYRLVADSHRENSVEIHSIRAVRSVAGSTVQPVPAYYGCRHDSHLGTLYWHARRIAGLTPNRLGSDLLLSLV
DTGFDPLREAPAFSLTAELLCTNRYLAERLGAGTALGFERPGPVAAARLRNAPSAQSQPRLDGQSRWRLVSQLTLNHLSL
VEGPRALEALREILALHNLRDDASARRQIEGLRQLDCQRVVAHVGEDAWRGWRNGLEIRLRLDPQHFVGASGVLFSAVLA
QFFSLYATANRFVRTVLVEADKEVKTWQPQAGSPLSL*					

Nucleotide sequence: 1794 bp

						>T6CP001471 NC_008463:c3026664-3024871 [Pseudomonas aeruginosa UCBPP-PA14] [TssF]
ATGAGCGATTCGATTGATGCCGACCTGCTCGACTACTACCAGCGCGAGCTGACCTGGCTACGGCACGCCGGCGGCGCCTT
CGCCGAGCGCTATCCGAAGGTCGCCCGGCGCCTGCAACTGGCCCCCGGCGAATGCCCCGACCCGCACGTCGAGCGTCTGC
TGGAAGGTTTCTCGCTGCTCACCGCGCGCCTGCAACGGCGCCTCGACGACGACTACGCAGAGTTCAGCGACGCCCTCCTC
GAACAGCTCTACCCCCTGGCGCTGCGCCCGCTGCCGTCCTGCGCGATCGTCCAGTTCGAACCGGACCCGACCCAGGGCAA
CCTCGCCGAGGGCTACCGGCTGCCGCGCGACACCGCGCTGTTCGTCACCACCCGTGGCGGCGACAGCGTGCATTTCCGCA
GCAGCGCCGAGGCGACCCTGTGGCCGCTGGCGATCGACGAGGCGACCCTGCTCGGCGGCGAGGAGGCACAAGCCCTGACC
GGAGTCGGCGCCGCCCGCTCGGCGTTGCGCCTGAGCCTGCGCTGCCTGGGCGAGTACCGCTGGGACGAACTGTCGATCGA
GCGCCTGCGCATCCACCTGGCGGCTTCGCCGATGGGCTGCGCGATCCTCTACGACCTGCTCGCCGCGCATGCCGTGCAGG
TCATGGCGGGGGCGCCCGGCACGCCGCCGGCGGCGCTCCGCGGACTGCCGGAGATCGTCGGCTTCGCCGCCGACGAGGCG
CTGCTGCCGGAGGAAGACGGCGGACATCCGGGCCTGCGCCTGCTCGCCGAGTACTTCGCCTTCCCCGACAAGTTCCACTT
CTTCGACCTGCCGCTGTCCGGCGCGCTCGCCGACGGACAGACGCTCTACCTGTACCTGGTCTTCGATCGCGCACCGACGG
CACGCCTGCATTTGCAGGCGGAAGACTTCCAGCTCGGCTGCGCGCCGGCGATCAACCTCTTTCCGCGGACCTCGGAGCCG
CTGCGCCCGGACGGCACCCGCAGCGAGTACCGCCTGGTCGCCGACAGTCACCGCGAGAACAGCGTGGAAATCCATAGCAT
CCGCGCCGTGCGCTCGGTTGCCGGGAGCACCGTGCAGCCAGTCCCGGCCTACTATGGCTGCCGCCACGACAGCCACCTCG
GCACGCTCTACTGGCACGCCCGGCGCATCGCCGGGCTGACCCCGAACCGGCTGGGCAGCGACCTGCTGCTGAGCCTGGTG
GATACCGGCTTCGACCCGTTGCGCGAGGCCCCGGCCTTCAGCCTGACCGCCGAGCTGCTATGCACCAATCGCTATCTGGC
CGAGCGCCTGGGTGCCGGCACCGCGCTGGGCTTCGAACGGCCCGGGCCGGTCGCCGCCGCACGCCTGCGCAACGCGCCCA
GCGCGCAGAGCCAGCCGCGCCTGGACGGCCAGTCGCGCTGGAGACTGGTCTCGCAGCTGACCCTCAACCACCTCTCGCTG
GTGGAAGGTCCGCGCGCCCTCGAAGCCCTGCGGGAAATCCTCGCCCTGCACAACCTGCGCGACGACGCCAGCGCCCGCCG
GCAGATAGAAGGGCTGCGGCAACTCGACTGCCAGCGGGTGGTGGCGCACGTCGGCGAGGATGCCTGGCGCGGCTGGCGCA
ATGGCCTGGAAATCCGCCTGCGACTCGATCCGCAACACTTCGTCGGCGCCAGCGGCGTGCTGTTCTCCGCCGTGCTGGCG
CAGTTCTTCTCGCTGTATGCCACCGCCAACCGCTTCGTGCGCACGGTCCTGGTGGAAGCCGACAAGGAGGTGAAGACATG
GCAACCCCAGGCCGGCTCGCCGCTCTCCCTCTGA					