Detailed information of component protein


Summary

Component ID T6CP001476
Component protein type TssM
T6SS ID (Type) T6SS00151 experimental (Type i4a)
Strain Pseudomonas aeruginosa UCBPP-PA14
Replicon chromosome
Sequence Protein sequence (1272 a.a.); Nucleotide sequence (3816 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PA14_RS13915
Coordinate (Strand) 3032557..3036372 (+)
NCBI ID WP_003139320.1
RefSeq NC_008463
Uniprot ID A0A0H2ZBB2_PSEAB
KEGG ID pau:PA14_34130
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 3.3e-48 115..351
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-4 MSGA
Transmembrane helix 5-27 TLFMLVLLLLLLALLLGALGWWW
Inside 28-348 RTRGGTEIRSFYAAVRQMEREQGWQGRYEAPWLLMLGNETEGEQLCATWRLLPVARPAWFGRWWSDGEGAILLVPESVFLPDEGLRRQSGAWLRLLRLFLRLRGRRALDGVVWNIPLARLQDGEQAAALGLAARRRYVELTQRLGLSLPVYVVITGLEDLPGFQELLAALPEEARERALGWSSPFAAEAAWQSRWCEQALEEITATLTESIVELGTLRGQVNNDLYCLPPRLESLRGSLQALLEPVFQGNARGEAPRFRGLYLSGSEAAGAASDEALPAVDAPRRRSSFASQLWARRILAEEGLAQAVPRILQLRQRWQRG
Transmembrane helix 349-368 IGLVALCLCLLWGGLMTWVW
Outside 369-1271 RDALRDAGELSQLLHGASERYQPLDDDTRRAAQVRQNVQAWWQLVAQAPRWRFTSLAFPSSWFSSLDARIDSAYRRVSERLLVRPLRSLLEGEASDLRAIRSDGQPGLKEGDDPSQWKDYLAAKDLVARATTLERHNRLFAQAIDNRKTPLDDLLQLSNDALGSSYNGGSLARLAYYNRTLFAERPADLAALDLGRVGATAADNFHDLMARWLNAYFLTDSLERTGNALTQELAQLGQERDATAAQLLGVGELINHLQQQVNRINLIWGNGVGQDLVPGYQNLLKSAQQSSLLGDRVEELQNLTVQLQQQFRSEWIAPPEAAGDGLLVKQGATLKLADDLLGLKRAIDDLKTKDFVSVALAGKTLAEHSLLSIDDIGVTQALSFFNDFSGYYESTLPSLNPRYRYSVLHAAASATTEAMWQSLGPRSRSLTSRNASRFDVQVKQVQALQAALLELQDMQAGARLLASLNALAVADIGQALRDIDEQAVLREPVDFSRWSGAPNFGLQAFRAQDSAELKQSLTSQFNAMAAVAERHAPALEWLQAQQSSLATADYNAFVRFSALNAELQKFKADNPASTAAQLAKLVGNDFNQMDIGSCADILNGVLLPSSRSDLATRLVDLRQGALGRCQSLQQQQAAQAWKDLADYFNQYLAGRFPFAYSLEAADAEPGRVRHLLKLMETRLPQVREGLAQVRSPDLPAAEDFVRRLEQAQRWLGPLFERDKSGLLGVALDIDWRSDRSLERGADQVIAWSLYSGDQESRFPGAQDKGLTWNVGDPLKIMLRWAKNGSQRPADDPRQASLAVADLEAGWSYQGSWALLRMMRAHFSRQRPPNVDYTEFPLVLQLPVYAPYSPENEARMFLRLSLMSLGGKTPLSIQPLPVRAPQSPFATLLPATVASTGGIP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9909 - -


  Protein sequence: 1272 a.a.    

>T6CP001476 NC_008463:3032557-3036372 [Pseudomonas aeruginosa UCBPP-PA14] [TssM]
MSGATLFMLVLLLLLLALLLGALGWWWRTRGGTEIRSFYAAVRQMEREQGWQGRYEAPWLLMLGNETEGEQLCATWRLLP
VARPAWFGRWWSDGEGAILLVPESVFLPDEGLRRQSGAWLRLLRLFLRLRGRRALDGVVWNIPLARLQDGEQAAALGLAA
RRRYVELTQRLGLSLPVYVVITGLEDLPGFQELLAALPEEARERALGWSSPFAAEAAWQSRWCEQALEEITATLTESIVE
LGTLRGQVNNDLYCLPPRLESLRGSLQALLEPVFQGNARGEAPRFRGLYLSGSEAAGAASDEALPAVDAPRRRSSFASQL
WARRILAEEGLAQAVPRILQLRQRWQRGIGLVALCLCLLWGGLMTWVWRDALRDAGELSQLLHGASERYQPLDDDTRRAA
QVRQNVQAWWQLVAQAPRWRFTSLAFPSSWFSSLDARIDSAYRRVSERLLVRPLRSLLEGEASDLRAIRSDGQPGLKEGD
DPSQWKDYLAAKDLVARATTLERHNRLFAQAIDNRKTPLDDLLQLSNDALGSSYNGGSLARLAYYNRTLFAERPADLAAL
DLGRVGATAADNFHDLMARWLNAYFLTDSLERTGNALTQELAQLGQERDATAAQLLGVGELINHLQQQVNRINLIWGNGV
GQDLVPGYQNLLKSAQQSSLLGDRVEELQNLTVQLQQQFRSEWIAPPEAAGDGLLVKQGATLKLADDLLGLKRAIDDLKT
KDFVSVALAGKTLAEHSLLSIDDIGVTQALSFFNDFSGYYESTLPSLNPRYRYSVLHAAASATTEAMWQSLGPRSRSLTS
RNASRFDVQVKQVQALQAALLELQDMQAGARLLASLNALAVADIGQALRDIDEQAVLREPVDFSRWSGAPNFGLQAFRAQ
DSAELKQSLTSQFNAMAAVAERHAPALEWLQAQQSSLATADYNAFVRFSALNAELQKFKADNPASTAAQLAKLVGNDFNQ
MDIGSCADILNGVLLPSSRSDLATRLVDLRQGALGRCQSLQQQQAAQAWKDLADYFNQYLAGRFPFAYSLEAADAEPGRV
RHLLKLMETRLPQVREGLAQVRSPDLPAAEDFVRRLEQAQRWLGPLFERDKSGLLGVALDIDWRSDRSLERGADQVIAWS
LYSGDQESRFPGAQDKGLTWNVGDPLKIMLRWAKNGSQRPADDPRQASLAVADLEAGWSYQGSWALLRMMRAHFSRQRPP
NVDYTEFPLVLQLPVYAPYSPENEARMFLRLSLMSLGGKTPLSIQPLPVRAPQSPFATLLPATVASTGGIP*

  Nucleotide sequence: 3816 bp    

>T6CP001476 NC_008463:3032557-3036372 [Pseudomonas aeruginosa UCBPP-PA14] [TssM]
ATGAGCGGCGCGACGCTGTTCATGCTGGTTCTCCTCCTGCTGCTGCTGGCGCTGCTGCTGGGGGCGCTGGGCTGGTGGTG
GCGGACCCGGGGCGGTACGGAGATCCGCAGCTTCTACGCGGCGGTCCGGCAGATGGAGCGCGAGCAGGGCTGGCAGGGCC
GCTACGAAGCGCCCTGGCTGCTGATGCTCGGCAACGAGACCGAGGGCGAGCAGTTGTGCGCTACCTGGCGCCTGCTGCCG
GTGGCGCGTCCGGCCTGGTTCGGCCGCTGGTGGTCGGACGGCGAGGGGGCGATCCTGCTGGTGCCCGAGTCGGTGTTCCT
GCCCGACGAGGGCCTGCGCCGGCAAAGCGGCGCCTGGCTGCGCCTGTTGCGTCTGTTCCTGCGCCTGCGCGGCAGGCGCG
CGCTGGACGGGGTGGTCTGGAACATTCCGCTGGCGCGGTTGCAGGATGGCGAGCAGGCGGCCGCCCTCGGCCTCGCCGCC
CGTCGTCGCTACGTGGAGCTGACCCAGCGCCTTGGCCTGAGCCTGCCGGTGTACGTGGTGATCACTGGCCTGGAAGACCT
GCCGGGTTTCCAGGAACTTCTCGCGGCGCTGCCCGAGGAGGCGCGCGAGCGCGCCCTCGGCTGGTCCTCGCCGTTCGCCG
CCGAGGCGGCCTGGCAGTCGCGCTGGTGCGAGCAGGCGCTGGAGGAAATCACCGCCACCCTGACCGAGTCGATCGTCGAA
CTCGGTACCTTGCGCGGCCAGGTGAACAACGACCTGTATTGCCTGCCGCCACGCCTGGAAAGCCTCCGCGGTTCGTTGCA
GGCGCTGCTCGAACCGGTCTTCCAGGGCAACGCCAGGGGTGAGGCGCCGCGCTTTCGCGGTCTCTACCTGAGCGGCAGCG
AGGCGGCGGGGGCGGCAAGCGACGAAGCCCTGCCGGCGGTCGACGCGCCGCGTCGGCGCAGCAGCTTCGCCAGCCAACTG
TGGGCACGGCGGATCCTTGCCGAGGAAGGCCTGGCCCAGGCGGTGCCGCGCATCCTCCAGCTGCGCCAGCGCTGGCAGCG
CGGTATCGGCCTCGTTGCGCTGTGCCTGTGCCTGCTCTGGGGCGGCCTGATGACCTGGGTCTGGCGTGACGCGCTGCGCG
ACGCCGGCGAACTGTCGCAACTGCTGCATGGCGCCAGCGAGCGCTACCAGCCGCTCGACGACGACACCCGGCGCGCCGCC
CAGGTCCGGCAGAACGTCCAGGCCTGGTGGCAACTGGTGGCACAGGCGCCGCGCTGGCGCTTCACCTCGCTGGCGTTCCC
CAGTTCCTGGTTTTCCTCCCTGGATGCACGTATCGACAGCGCGTATCGCCGCGTTTCCGAGCGCCTGCTGGTGCGCCCGC
TGCGCAGCCTGCTGGAAGGCGAGGCGAGCGACCTGCGGGCGATCCGCAGCGACGGCCAGCCCGGCCTGAAGGAGGGCGAC
GACCCCAGCCAGTGGAAGGACTACCTGGCGGCGAAGGACCTGGTCGCCCGCGCCACTACCCTGGAGCGCCACAATCGCCT
GTTCGCCCAGGCCATCGACAACCGCAAGACGCCGCTCGACGATCTCCTGCAACTGAGCAACGACGCCCTGGGCAGCAGCT
ACAACGGCGGCAGCCTGGCGCGCCTGGCCTACTACAACCGCACGCTGTTCGCCGAGCGTCCGGCCGATCTCGCCGCCCTC
GACCTGGGCCGGGTCGGCGCCACGGCGGCGGATAACTTCCATGACCTGATGGCACGCTGGCTGAACGCCTACTTTCTCAC
CGACAGCCTGGAACGCACCGGCAATGCCCTGACCCAGGAACTCGCGCAGCTCGGCCAGGAACGAGATGCCACGGCGGCCC
AGTTGCTGGGGGTCGGCGAATTGATCAACCACCTGCAGCAGCAGGTCAACCGCATCAACCTGATCTGGGGCAATGGCGTC
GGCCAGGATCTCGTCCCTGGCTACCAGAACCTGCTCAAGAGCGCCCAGCAAAGCAGCCTGCTGGGAGATCGCGTGGAGGA
ACTGCAGAACCTCACGGTGCAGTTGCAGCAGCAGTTCCGCAGCGAATGGATCGCGCCTCCGGAGGCTGCCGGCGACGGCC
TGCTGGTGAAACAGGGTGCGACGCTCAAGCTGGCCGATGACCTGCTCGGCCTGAAACGCGCCATCGATGACCTGAAGACC
AAGGACTTCGTCTCCGTGGCCCTGGCCGGCAAGACACTGGCGGAGCACTCCTTGCTGAGCATCGACGACATCGGCGTGAC
TCAGGCGTTGAGCTTCTTCAACGACTTCAGCGGCTACTACGAGAGCACCCTGCCGTCGCTCAACCCGCGCTATCGCTACT
CGGTCCTGCACGCCGCCGCCAGCGCGACGACCGAGGCCATGTGGCAGAGCCTCGGGCCGCGCAGTCGCAGCCTGACCAGC
CGCAATGCCTCGCGCTTCGACGTGCAGGTCAAGCAGGTCCAGGCATTGCAGGCGGCGCTGCTGGAGTTGCAGGACATGCA
GGCCGGCGCGCGCCTGCTGGCCAGCCTCAACGCGCTGGCCGTGGCCGATATCGGCCAGGCCCTGCGCGACATCGACGAGC
AGGCGGTGCTGCGCGAGCCGGTCGATTTCAGCCGTTGGTCGGGCGCGCCGAATTTCGGCCTGCAGGCGTTCCGCGCCCAG
GACAGCGCCGAGCTGAAGCAGAGCCTGACCAGCCAGTTCAATGCCATGGCCGCGGTCGCCGAGCGCCACGCCCCGGCGCT
GGAGTGGCTGCAGGCGCAGCAGTCCAGCCTGGCCACCGCCGACTACAACGCGTTCGTGCGCTTCAGCGCGCTCAACGCCG
AACTGCAGAAATTCAAGGCCGACAACCCGGCGAGCACCGCGGCGCAGCTGGCGAAGCTGGTGGGTAACGATTTCAACCAG
ATGGACATCGGCAGTTGCGCCGACATTCTCAACGGCGTGCTCCTGCCGTCCAGCCGCAGCGACCTGGCGACGCGCCTGGT
GGACCTGCGCCAGGGCGCGCTCGGGCGCTGCCAGTCGTTGCAACAGCAGCAGGCGGCGCAGGCCTGGAAGGACCTCGCCG
ACTACTTCAACCAGTACCTCGCCGGACGCTTCCCGTTCGCCTACAGCCTGGAAGCGGCCGATGCCGAGCCGGGGCGGGTG
CGGCACCTGCTGAAGCTGATGGAGACGCGCTTGCCGCAGGTGCGCGAAGGGCTGGCCCAGGTTCGCTCGCCGGACCTGCC
GGCCGCCGAGGACTTCGTCCGCCGCCTGGAGCAGGCGCAGCGCTGGCTCGGCCCGCTGTTCGAGCGCGACAAGTCGGGCC
TGCTCGGCGTCGCCCTGGACATCGACTGGCGCAGCGACCGCAGCCTGGAACGCGGCGCCGACCAGGTGATCGCCTGGAGC
CTGTACTCGGGCGACCAGGAAAGCCGGTTCCCCGGCGCGCAGGACAAGGGCCTGACCTGGAACGTCGGCGATCCGCTGAA
GATCATGCTGCGCTGGGCGAAGAACGGCTCGCAGCGTCCGGCAGACGACCCGCGCCAGGCCAGCCTGGCGGTGGCCGACC
TCGAGGCCGGCTGGAGCTACCAGGGCTCCTGGGCGCTGCTGCGCATGATGCGTGCGCACTTCTCCCGGCAGCGCCCGCCG
AACGTCGACTACACGGAGTTTCCGCTGGTCCTGCAACTGCCGGTGTATGCGCCCTACAGCCCGGAAAACGAGGCGCGGAT
GTTCCTCCGCCTGTCGTTGATGAGCCTGGGCGGCAAGACCCCGCTGTCCATCCAGCCGCTGCCGGTGCGCGCGCCGCAAT
CGCCGTTCGCGACGCTGTTGCCGGCCACCGTCGCCAGCACCGGAGGTATCCCATGA