Detailed information of regulator
Summary
| Regulator ID | REG0167  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | QS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00152 (Type i1); T6SS00151 (Type i4a); T6SS00150 (Type i3) |
| Strain | Pseudomonas aeruginosa UCBPP-PA14 |
| Replicon | chromosome |
| Sequence | Protein sequence (333 a.a.); Nucleotide sequence (999 bp) |
| Description | Transcription of hcp2 and hcp3 was affected in the QS mutant ΔmvfR, demonstrating that both MvfR control hcp2 and hcp3 expression. In contrast, hcp1 transcript levels were maintained at low levels of expression, which increased in ΔmvfR mutant, suggesting that MvfR repress the HSI-I effector hcp1. |
| Reference |
External database links
| Locus tag (Gene) | PA14_51340 (MvfR) |
| Coordinates (Strand) | 4562837..4563835 (+) |
| NCBI ID | 489176730 |
| RefSeq | NC_008463 |
| Uniprot ID | V6AIV4_PSEAI, A0A1C7BZI4_PSEAI, A0A0H2Z7A6_PSEAB, A0A1C6ZJJ6_PSEAI |
| KEGG ID | pau:PA14_51340 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | 4JVC, 4JVD, 6Z17, 6Z5K, 4JVI, 6Z07, 6TPR, 6YZ3 |
Target
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Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| Upstream of pqs operon | ↓ | Putative MvfR binding sites using the only known MvfR binding site to date : TTCGGACTCCGAA |
| HSI-III transcripts | ↓ | - |
| HSI-I transcripts | ↑ | - |
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Pfam domain hit(s) of regulator
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Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| LysR_substrate | PF03466.22 | 2.7e-14 | 139..292 |
| HTH_1 | PF00126.29 | 1.7e-10 | 6..64 |
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Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
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Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-332 | MPIHNLNHVNMFLQVIASGSISSAARILRKSHTAVSSAVSNLEIDLCVELVRRDGYKVEPTEQALRLIPYMRSLLNYQQLIGDIAFNLNKGPRNLRVLLDTAIPPSFCDTVSSVLLDDFNMVSLIRTSPADSLATIKQDNAEIDIAITIDEELKISRFNQCVLGYTKAFVVAHPQHPLCNASLHSIASLANYRQISLGSRSGQHSNLLRPVSDKVLFVENFDDMLRLVEAGVGWGIAPHYFVEERLRNGTLAVLSELYEPGGIDTKVYCYYNTALESERSFLRFLESARQRLRELGRQRFDDAPAWQPSIVETVQRRSGPKALAYRQRAAPE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
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Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.6122 | - | - |
Protein sequence of regulator: 333 a.a. .
>REG0167 NC_008463:4562837-4563835 [Pseudomonas aeruginosa UCBPP-PA14]
MPIHNLNHVNMFLQVIASGSISSAARILRKSHTAVSSAVSNLEIDLCVELVRRDGYKVEPTEQALRLIPYMRSLLNYQQL
IGDIAFNLNKGPRNLRVLLDTAIPPSFCDTVSSVLLDDFNMVSLIRTSPADSLATIKQDNAEIDIAITIDEELKISRFNQ
CVLGYTKAFVVAHPQHPLCNASLHSIASLANYRQISLGSRSGQHSNLLRPVSDKVLFVENFDDMLRLVEAGVGWGIAPHY
FVEERLRNGTLAVLSELYEPGGIDTKVYCYYNTALESERSFLRFLESARQRLRELGRQRFDDAPAWQPSIVETVQRRSGP
KALAYRQRAAPE*
MPIHNLNHVNMFLQVIASGSISSAARILRKSHTAVSSAVSNLEIDLCVELVRRDGYKVEPTEQALRLIPYMRSLLNYQQL
IGDIAFNLNKGPRNLRVLLDTAIPPSFCDTVSSVLLDDFNMVSLIRTSPADSLATIKQDNAEIDIAITIDEELKISRFNQ
CVLGYTKAFVVAHPQHPLCNASLHSIASLANYRQISLGSRSGQHSNLLRPVSDKVLFVENFDDMLRLVEAGVGWGIAPHY
FVEERLRNGTLAVLSELYEPGGIDTKVYCYYNTALESERSFLRFLESARQRLRELGRQRFDDAPAWQPSIVETVQRRSGP
KALAYRQRAAPE*
Nucleotide sequence of regulator: 999 bp .
>REG0167 NC_008463:4562837-4563835 [Pseudomonas aeruginosa UCBPP-PA14]
ATGCCTATTCATAACCTGAATCACGTGAACATGTTCCTCCAGGTCATCGCCTCCGGTTCGATTTCCTCCGCTGCGCGGAT
CCTGCGCAAGTCGCACACCGCGGTCAGCTCGGCGGTCAGCAACCTGGAAATCGACCTGTGCGTGGAGCTGGTCCGTCGGG
ACGGCTACAAGGTCGAACCCACCGAGCAGGCGCTTCGCCTGATCCCTTACATGCGCAGCCTGCTGAACTACCAGCAGCTG
ATCGGCGACATCGCCTTCAATCTCAACAAGGGTCCGCGCAATCTCCGGGTGCTGCTGGACACCGCCATCCCGCCGTCGTT
CTGCGATACGGTGAGCAGCGTACTGCTCGACGATTTCAACATGGTCAGCCTGATACGCACCTCGCCCGCCGATAGCCTGG
CGACGATCAAGCAGGACAACGCGGAAATCGATATCGCCATCACCATCGACGAGGAACTGAAGATCTCCCGCTTCAACCAG
TGCGTGCTCGGCTACACCAAGGCGTTCGTCGTCGCCCATCCGCAGCACCCGTTGTGCAATGCCTCCCTGCACAGCATCGC
GAGCCTGGCCAATTACCGGCAGATCAGCCTCGGCAGCCGCTCCGGGCAGCATTCGAACCTGCTGCGGCCGGTCAGCGACA
AGGTGCTCTTCGTGGAAAACTTCGACGACATGCTGCGTCTGGTGGAAGCCGGCGTCGGATGGGGCATCGCGCCGCATTAT
TTCGTCGAGGAACGCCTGCGCAACGGTACCCTGGCAGTCCTCAGCGAACTCTACGAACCGGGCGGCATCGACACCAAGGT
GTATTGCTACTACAACACCGCGCTGGAATCCGAGCGCAGCTTCCTGCGCTTTCTCGAAAGCGCCCGCCAGCGCCTGCGCG
AACTGGGCCGCCAGCGTTTCGACGATGCGCCGGCCTGGCAACCGAGCATCGTCGAAACGGTGCAGCGCCGCTCAGGCCCG
AAGGCGCTCGCGTACCGCCAGCGCGCCGCACCAGAGTAG
ATGCCTATTCATAACCTGAATCACGTGAACATGTTCCTCCAGGTCATCGCCTCCGGTTCGATTTCCTCCGCTGCGCGGAT
CCTGCGCAAGTCGCACACCGCGGTCAGCTCGGCGGTCAGCAACCTGGAAATCGACCTGTGCGTGGAGCTGGTCCGTCGGG
ACGGCTACAAGGTCGAACCCACCGAGCAGGCGCTTCGCCTGATCCCTTACATGCGCAGCCTGCTGAACTACCAGCAGCTG
ATCGGCGACATCGCCTTCAATCTCAACAAGGGTCCGCGCAATCTCCGGGTGCTGCTGGACACCGCCATCCCGCCGTCGTT
CTGCGATACGGTGAGCAGCGTACTGCTCGACGATTTCAACATGGTCAGCCTGATACGCACCTCGCCCGCCGATAGCCTGG
CGACGATCAAGCAGGACAACGCGGAAATCGATATCGCCATCACCATCGACGAGGAACTGAAGATCTCCCGCTTCAACCAG
TGCGTGCTCGGCTACACCAAGGCGTTCGTCGTCGCCCATCCGCAGCACCCGTTGTGCAATGCCTCCCTGCACAGCATCGC
GAGCCTGGCCAATTACCGGCAGATCAGCCTCGGCAGCCGCTCCGGGCAGCATTCGAACCTGCTGCGGCCGGTCAGCGACA
AGGTGCTCTTCGTGGAAAACTTCGACGACATGCTGCGTCTGGTGGAAGCCGGCGTCGGATGGGGCATCGCGCCGCATTAT
TTCGTCGAGGAACGCCTGCGCAACGGTACCCTGGCAGTCCTCAGCGAACTCTACGAACCGGGCGGCATCGACACCAAGGT
GTATTGCTACTACAACACCGCGCTGGAATCCGAGCGCAGCTTCCTGCGCTTTCTCGAAAGCGCCCGCCAGCGCCTGCGCG
AACTGGGCCGCCAGCGTTTCGACGATGCGCCGGCCTGGCAACCGAGCATCGTCGAAACGGTGCAGCGCCGCTCAGGCCCG
AAGGCGCTCGCGTACCGCCAGCGCGCCGCACCAGAGTAG