Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00152 experimental
T6SS type Type i1
Strain Pseudomonas aeruginosa UCBPP-PA14
Replicon (RefSeq) chromosome (NC_008463)
Location 3806993..3840213
Function anti-eukaryotic; anti-bacterial; virulence
Comment Needed for full virulence in mouse lung and acute burn model; required for wild-type infection of Arabidopsis; A Rhs element and the cognate immunity protein are involved in the bacterial killing.
References
[1] Hachani A et al (2014) The VgrG proteins are "A la carte" delivery systems for bacterial type VI effectors. J Biol Chem. 289(25):17872-17884. [PudMed:24794869]
[2] Lesic B et al (2009) Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis. Microbiology. 155(Pt 9):2845-55. [PudMed:19497948]
[3] Barret M et al (2011) Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537]
[4] Jones C et al (2013) An rhs-encoding gene linked to the second type VI secretion cluster is a feature of the Pseudomonas aeruginosa strain PA14. J Bacteriol. 196(4):800-10. [PudMed:24317402]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentFHASfa2Stk1Stp1TssATssBTssCTssDTssETssFTssGTssHTssITssJTssKTssLTssM
Number11111111111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
PA14_RS17365 (aroC) 3802844..3803935 - 1092 WP_003087653.1 chorismate synthase
PA14_RS17370 3804043..3805026 + 984 WP_003140073.1 alpha/beta hydrolase
PA14_RS17375 3805104..3805883 + 780 WP_003087649.1 hypothetical protein
PA14_RS17380 (prmB) 3805908..3806822 - 915 WP_003087648.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase
PA14_RS17385 3806993..3807589 + 597 WP_003140074.1 cysteine hydrolase
PA14_RS17390 3807762..3808082 + 321 WP_003140075.1 hypothetical protein
PA14_RS17395 3808139..3808696 + 558 WP_003140076.1 Smr/MutS family protein
PA14_RS17400 (folE) 3808774..3809319 + 546 WP_003087639.1 GTP cyclohydrolase I FolE
PA14_RS17405 3809384..3809845 - 462 WP_003087637.1 bacteriohemerythrin
PA14_RS17410 3810092..3810472 + 381 WP_003110435.1 VOC family protein
T6CP012463 Stk1 PA14_RS17415 3810500..3811489 - 990 WP_003140077.1 serine/threonine protein kinase
T6CP012462 Stp1 PA14_RS17420 3811486..3812214 - 729 WP_003110437.1 serine/threonine-protein phosphatase
T6CP001478 TssM PA14_RS17425 (tssM) 3812214..3815741 - 3528 WP_003140078.1 type VI secretion system membrane subunit TssM
T6CP001479 TssL PA14_RS17430 3815757..3816626 - 870 WP_003120354.1 type IVB secretion system protein IcmH/DotU
T6CP001480 TssK PA14_RS17435 (tssK) 3816629..3817960 - 1332 WP_003087620.1 type VI secretion system baseplate subunit TssK
T6CP001481 TssJ PA14_RS17440 (tssJ) 3817957..3818463 - 507 WP_003110438.1 type VI secretion system lipoprotein TssJ
T6CP011365 FHA PA14_RS17445 (tagH) 3818469..3819662 - 1194 WP_003140079.1 type VI secretion system-associated FHA domain protein TagH
PA14_RS17450 3819680..3819820 - 141 WP_003087614.1 hypothetical protein
T6CP012461 Sfa2 PA14_RS17455 3819909..3821420 - 1512 WP_003140081.1 sigma-54-dependent Fis family transcriptional regulator
T6CP001482 TssH PA14_RS17460 (tssH) 3821431..3824064 - 2634 WP_003140083.1 type VI secretion system ATPase TssH
T6CP001483 TssG PA14_RS17465 (tssG) 3824071..3825078 - 1008 WP_003140086.1 type VI secretion system baseplate subunit TssG
T6CP001484 TssF PA14_RS17470 (tssF) 3825042..3826829 - 1788 WP_003087605.1 type VI secretion system baseplate subunit TssF
PA14_RS31155 3826989..3827156 - 168 pseudogene PAAR domain-containing protein
T6CP009592 TssE PA14_RS17475 (tssE) 3827167..3827574 - 408 WP_003140089.1 type VI secretion system baseplate subunit TssE
T6CP001485 TssC PA14_RS17480 (tssC) 3827587..3829062 - 1476 WP_003087596.1 type VI secretion system contractile sheath large subunit
T6CP001486 TssB PA14_RS17485 (tssB) 3829090..3829596 - 507 WP_003087592.1 type VI secretion system contractile sheath small subunit
T6CP009591 TssA PA14_RS17490 (tssA) 3829632..3831188 - 1557 WP_003140090.1 type VI secretion system protein TssA
T6CP009590 TssD PA14_RS17495 3831886..3832404 + 519 WP_003083317.1 Hcp family type VI secretion system effector
T6CP001487 TssI PA14_RS17500 3832600..3834642 + 2043 WP_011666709.1 type VI secretion system tip protein VgrG
PA14_RS17505 3834632..3835372 + 741 WP_003140105.1 DUF4123 domain-containing protein
PA14_RS17510 3835369..3840213 + 4845 WP_003140107.1 RHS repeat protein
PA14_RS30650 3840223..3840657 + 435 WP_016254153.1 hypothetical protein
PA14_RS17515 3841020..3841622 - 603 WP_003097816.1 glutathione S-transferase family protein
PA14_RS17520 3841678..3842844 - 1167 WP_003140110.1 PLP-dependent aminotransferase family protein
PA14_RS17525 3842926..3843402 + 477 WP_003097819.1 MarR family transcriptional regulator
PA14_RS17530 3843452..3844087 - 636 WP_003087573.1 hypothetical protein
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0166 experimental PA14_45960 (lasR) protein QS regulatory pathway Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 4085339..4086058 (-) 489173455 TssA2 (PA14_43050) promotor (↓); HSI-III transcripts (↓); HSI-I transcripts (↑)
REG0167 experimental PA14_51340 (MvfR) protein QS regulatory pathway Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 4562837..4563835 (+) 489176730 Upstream of pqs operon (↓); HSI-III transcripts (↓); HSI-I transcripts (↑)
REG0168 experimental PA14_51380 (pqsE) protein QS regulatory pathway Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 4566077..4566982 (-) 489176725 T6SS Transciption (↓)
REG0169 experimental PA14_22470 (PA3225) protein Global regulator Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 1955624..1956553 (-) 489182081 PA14_00990 (TssA) promotor (↓); TssA1 (PA14_00990) transcript (↑); TssA2 (PA14_43050) transcript (↑); TssB3 (PA14_34070) transcript (↑)
REG0170 experimental PA14_20290 (AmrZ) protein Global regulator Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 1747602..1747928 (-) 489182353 TssA1 (PA14_00990) promotor (↓); TssA2 (PA14_43050) promotor (↑); TssB3 (PA14_34070) promotor (↓)
REG0172 experimental PA14_48150 (QslA) protein QS regulatory pathway Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 4286222..4286536 (+) 489177206 H2-T6SS (PA14_43050) promotor (↑)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF00186 experimental PA14_03240 (hcp2) - - Pseudomonas aeruginosa UCBPP-PA14 chromosome (NC_008463) 288454..288972 (-) 116053976

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector