Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00152  |
| T6SS type | Type i1 |
| Strain | Pseudomonas aeruginosa UCBPP-PA14 |
| Replicon (RefSeq) | chromosome (NC_008463) |
| Location | 3806993..3840213 |
| Function | anti-eukaryotic; anti-bacterial; virulence |
| Comment | Needed for full virulence in mouse lung and acute burn model; required for wild-type infection of Arabidopsis; A Rhs element and the cognate immunity protein are involved in the bacterial killing. |
| References | [1] Hachani A et al (2014) The VgrG proteins are "A la carte" delivery systems for bacterial type VI effectors. J Biol Chem. 289(25):17872-17884. [PudMed:24794869] [2] Lesic B et al (2009) Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis. Microbiology. 155(Pt 9):2845-55. [PudMed:19497948] [3] Barret M et al (2011) Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537] [4] Jones C et al (2013) An rhs-encoding gene linked to the second type VI secretion cluster is a feature of the Pseudomonas aeruginosa strain PA14. J Bacteriol. 196(4):800-10. [PudMed:24317402] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | FHA | Sfa2 | Stk1 | Stp1 | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| PA14_RS17365 (aroC) | 3802844..3803935 | - | 1092 | WP_003087653.1 | chorismate synthase | ||
| PA14_RS17370 | 3804043..3805026 | + | 984 | WP_003140073.1 | alpha/beta hydrolase | ||
| PA14_RS17375 | 3805104..3805883 | + | 780 | WP_003087649.1 | hypothetical protein | ||
| PA14_RS17380 (prmB) | 3805908..3806822 | - | 915 | WP_003087648.1 | 50S ribosomal protein L3 N(5)-glutamine methyltransferase | ||
| PA14_RS17385 | 3806993..3807589 | + | 597 | WP_003140074.1 | cysteine hydrolase | ||
| PA14_RS17390 | 3807762..3808082 | + | 321 | WP_003140075.1 | hypothetical protein | ||
| PA14_RS17395 | 3808139..3808696 | + | 558 | WP_003140076.1 | Smr/MutS family protein | ||
| PA14_RS17400 (folE) | 3808774..3809319 | + | 546 | WP_003087639.1 | GTP cyclohydrolase I FolE | ||
| PA14_RS17405 | 3809384..3809845 | - | 462 | WP_003087637.1 | bacteriohemerythrin | ||
| PA14_RS17410 | 3810092..3810472 | + | 381 | WP_003110435.1 | VOC family protein | ||
| T6CP012463 | Stk1 | PA14_RS17415 | 3810500..3811489 | - | 990 | WP_003140077.1 | serine/threonine protein kinase |
| T6CP012462 | Stp1 | PA14_RS17420 | 3811486..3812214 | - | 729 | WP_003110437.1 | serine/threonine-protein phosphatase |
| T6CP001478 | TssM | PA14_RS17425 (tssM) | 3812214..3815741 | - | 3528 | WP_003140078.1 | type VI secretion system membrane subunit TssM |
| T6CP001479 | TssL | PA14_RS17430 | 3815757..3816626 | - | 870 | WP_003120354.1 | type IVB secretion system protein IcmH/DotU |
| T6CP001480 | TssK | PA14_RS17435 (tssK) | 3816629..3817960 | - | 1332 | WP_003087620.1 | type VI secretion system baseplate subunit TssK |
| T6CP001481 | TssJ | PA14_RS17440 (tssJ) | 3817957..3818463 | - | 507 | WP_003110438.1 | type VI secretion system lipoprotein TssJ |
| T6CP011365 | FHA | PA14_RS17445 (tagH) | 3818469..3819662 | - | 1194 | WP_003140079.1 | type VI secretion system-associated FHA domain protein TagH |
| PA14_RS17450 | 3819680..3819820 | - | 141 | WP_003087614.1 | hypothetical protein | ||
| T6CP012461 | Sfa2 | PA14_RS17455 | 3819909..3821420 | - | 1512 | WP_003140081.1 | sigma-54-dependent Fis family transcriptional regulator |
| T6CP001482 | TssH | PA14_RS17460 (tssH) | 3821431..3824064 | - | 2634 | WP_003140083.1 | type VI secretion system ATPase TssH |
| T6CP001483 | TssG | PA14_RS17465 (tssG) | 3824071..3825078 | - | 1008 | WP_003140086.1 | type VI secretion system baseplate subunit TssG |
| T6CP001484 | TssF | PA14_RS17470 (tssF) | 3825042..3826829 | - | 1788 | WP_003087605.1 | type VI secretion system baseplate subunit TssF |
| PA14_RS31155 | 3826989..3827156 | - | 168 | pseudogene | PAAR domain-containing protein | ||
| T6CP009592 | TssE | PA14_RS17475 (tssE) | 3827167..3827574 | - | 408 | WP_003140089.1 | type VI secretion system baseplate subunit TssE |
| T6CP001485 | TssC | PA14_RS17480 (tssC) | 3827587..3829062 | - | 1476 | WP_003087596.1 | type VI secretion system contractile sheath large subunit |
| T6CP001486 | TssB | PA14_RS17485 (tssB) | 3829090..3829596 | - | 507 | WP_003087592.1 | type VI secretion system contractile sheath small subunit |
| T6CP009591 | TssA | PA14_RS17490 (tssA) | 3829632..3831188 | - | 1557 | WP_003140090.1 | type VI secretion system protein TssA |
| T6CP009590 | TssD | PA14_RS17495 | 3831886..3832404 | + | 519 | WP_003083317.1 | Hcp family type VI secretion system effector |
| T6CP001487 | TssI | PA14_RS17500 | 3832600..3834642 | + | 2043 | WP_011666709.1 | type VI secretion system tip protein VgrG |
| PA14_RS17505 | 3834632..3835372 | + | 741 | WP_003140105.1 | DUF4123 domain-containing protein | ||
| PA14_RS17510 | 3835369..3840213 | + | 4845 | WP_003140107.1 | RHS repeat protein | ||
| PA14_RS30650 | 3840223..3840657 | + | 435 | WP_016254153.1 | hypothetical protein | ||
| PA14_RS17515 | 3841020..3841622 | - | 603 | WP_003097816.1 | glutathione S-transferase family protein | ||
| PA14_RS17520 | 3841678..3842844 | - | 1167 | WP_003140110.1 | PLP-dependent aminotransferase family protein | ||
| PA14_RS17525 | 3842926..3843402 | + | 477 | WP_003097819.1 | MarR family transcriptional regulator | ||
| PA14_RS17530 | 3843452..3844087 | - | 636 | WP_003087573.1 | hypothetical protein |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed. Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF00186 |
PA14_03240 (hcp2) | - | - | Pseudomonas aeruginosa UCBPP-PA14 | chromosome (NC_008463) | 288454..288972 (-) | 116053976 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
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