Detailed information of component protein


Summary

Component ID T6CP001479
Component protein type TssL
T6SS ID (Type) T6SS00152 experimental (Type i1)
Strain Pseudomonas aeruginosa UCBPP-PA14
Replicon chromosome
Sequence Protein sequence (290 a.a.); Nucleotide sequence (870 bp)
Reference
[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
[2] Lesic B, Starkey M, He J, et al. Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis. Microbiology (Reading). 2009 Sep;155(Pt 9):2845-2855. doi: 10.1099/mic.0.029082-0. Epub 2009 Jun 4. PMID: 19497948
[3] Hachani A, Allsopp LP, Oduko Y, et al. The VgrG proteins are "à la carte" delivery systems for bacterial type VI effectors. J Biol Chem. 2014 Jun 20;289(25):17872-84. doi: 10.1074/jbc.M114.563429. Epub 2014 May 2. PMID: 24794869
[4] Jones C, Hachani A, Manoli E, et al. An rhs gene linked to the second type VI secretion cluster is a feature of the Pseudomonas aeruginosa strain PA14. J Bacteriol. 2014 Feb;196(4):800-10. doi: 10.1128/JB.00863-13. Epub 2013 Dec 6. PMID: 24317402
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PA14_RS17430
Coordinate (Strand) 3815757..3816626 (-)
NCBI ID WP_003120354.1
RefSeq NC_008463
Uniprot ID A0A0H2Z9C9_PSEAB, A0A0C6EWN9_PSEAI
KEGG ID pau:PA14_42910
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 3.1e-66 62..264
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-243 MIKEREYHEDEKTVLLDHQGQVPAQGPLTDFAEPPRFEQLEERMIYAARLRPGEAFNVSLNPLVAAASELLSEVVRLKHSSVLENLAALKERLTSAMKLFEFRAVQDGVENSQVMAARYVLCTVVDEAVVTTPWGNESEWSKISLLSSFHNETFGGEKFFQLLDRLSKNPVKHLPMLELMYLCLALGFEGKYRVMARGVLDLDGISDALYRQIRQLRGDVPRELSPHWQGLSDQRRGLVRIVP
Transmembrane helix 244-266 WWMVALFTLVCLVVMYSGFAWVL
Inside 267-289 GEQRDTVLQPYQSLDAAAGQPGS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9871 - -


  Protein sequence: 290 a.a.    

>T6CP001479 NC_008463:c3816626-3815757 [Pseudomonas aeruginosa UCBPP-PA14] [TssL]
MIKEREYHEDEKTVLLDHQGQVPAQGPLTDFAEPPRFEQLEERMIYAARLRPGEAFNVSLNPLVAAASELLSEVVRLKHS
SVLENLAALKERLTSAMKLFEFRAVQDGVENSQVMAARYVLCTVVDEAVVTTPWGNESEWSKISLLSSFHNETFGGEKFF
QLLDRLSKNPVKHLPMLELMYLCLALGFEGKYRVMARGVLDLDGISDALYRQIRQLRGDVPRELSPHWQGLSDQRRGLVR
IVPWWMVALFTLVCLVVMYSGFAWVLGEQRDTVLQPYQSLDAAAGQPGS*

  Nucleotide sequence: 870 bp    

>T6CP001479 NC_008463:c3816626-3815757 [Pseudomonas aeruginosa UCBPP-PA14] [TssL]
ATGATCAAGGAAAGGGAATACCACGAGGACGAGAAGACCGTCCTCCTCGATCACCAGGGACAGGTACCGGCCCAGGGGCC
GCTGACCGATTTCGCCGAACCGCCGCGCTTCGAGCAGCTCGAGGAGCGGATGATCTACGCGGCGCGCCTGCGTCCGGGCG
AGGCCTTCAACGTCAGCCTGAACCCGCTGGTGGCGGCGGCTTCCGAACTGCTTTCGGAAGTGGTCCGGCTCAAGCACAGC
AGCGTGCTGGAGAACCTTGCGGCGCTCAAGGAGCGGTTGACCTCCGCCATGAAGCTCTTCGAATTCCGCGCCGTGCAGGA
TGGCGTGGAAAACAGCCAGGTCATGGCGGCGCGCTACGTGCTGTGCACGGTGGTCGACGAGGCGGTGGTGACCACCCCGT
GGGGCAACGAAAGCGAATGGTCGAAGATCAGCCTGCTGAGCAGCTTCCATAACGAGACCTTCGGCGGCGAGAAGTTCTTC
CAGTTGCTCGACCGGCTGTCGAAGAACCCGGTCAAGCATCTGCCGATGCTGGAGCTGATGTACCTCTGCCTGGCCCTCGG
CTTCGAGGGCAAGTACCGGGTGATGGCTCGCGGCGTGCTCGACCTGGACGGTATTTCCGATGCGCTCTACCGGCAGATCC
GCCAGTTGCGCGGCGACGTGCCGCGCGAACTGTCGCCGCACTGGCAGGGGCTCAGCGACCAGCGTCGCGGCCTGGTGCGG
ATCGTGCCCTGGTGGATGGTGGCGCTGTTCACCCTGGTCTGCCTGGTGGTGATGTATTCGGGATTCGCCTGGGTTCTCGG
CGAGCAACGCGACACGGTGCTGCAGCCTTACCAGTCGCTCGATGCGGCGGCAGGACAGCCCGGTTCGTAA