Detailed information of regulator
Summary
| Regulator ID | REG0172  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | QS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00152 (Type i1) |
| Strain | Pseudomonas aeruginosa UCBPP-PA14 |
| Replicon | chromosome |
| Sequence | Protein sequence (105 a.a.); Nucleotide sequence (315 bp) |
| Description | The expression of H2-T6SS machinery genes was controlled at a transcriptional level by QslA. |
| Reference |
External database links
| Locus tag (Gene) | PA14_48150 (QslA) |
| Coordinates (Strand) | 4286222..4286536 (+) |
| NCBI ID | 489177206 |
| RefSeq | NC_008463 |
| Uniprot ID | A0A071L909_PSEAI |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| H2-T6SS (PA14_43050) promotor | ↑ | 489210784 |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| QslA_E | PF18226.3 | 1.2e-37 | 27..97 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-104 | MTKDEKEKTHVDAIIERYKDLMVEIPPADRQPGLSLLWPVPAQPAIDKGVRQAENWLADQIEGQLWTAFAFGRDSLPTPMQKTAFEVAFLTRLQQRLVAARRSG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9866 | - | - |
Protein sequence of regulator: 105 a.a. .
>REG0172 NC_008463:4286222-4286536 [Pseudomonas aeruginosa UCBPP-PA14]
MTKDEKEKTHVDAIIERYKDLMVEIPPADRQPGLSLLWPVPAQPAIDKGVRQAENWLADQIEGQLWTAFAFGRDSLPTPM
QKTAFEVAFLTRLQQRLVAARRSG*
MTKDEKEKTHVDAIIERYKDLMVEIPPADRQPGLSLLWPVPAQPAIDKGVRQAENWLADQIEGQLWTAFAFGRDSLPTPM
QKTAFEVAFLTRLQQRLVAARRSG*
Nucleotide sequence of regulator: 315 bp .
>REG0172 NC_008463:4286222-4286536 [Pseudomonas aeruginosa UCBPP-PA14]
ATGACGAAGGATGAAAAGGAAAAGACCCACGTCGACGCAATCATCGAACGCTACAAGGACCTGATGGTCGAGATTCCCCC
CGCCGACCGGCAACCCGGTCTCTCCCTGCTCTGGCCGGTTCCCGCCCAGCCCGCCATCGACAAGGGCGTCCGCCAGGCCG
AGAACTGGCTTGCCGACCAGATCGAAGGCCAGCTCTGGACCGCCTTCGCCTTCGGCCGCGACAGCCTGCCCACACCGATG
CAGAAGACCGCCTTCGAAGTGGCCTTCCTGACGCGCCTGCAGCAGCGCCTGGTAGCCGCTCGACGTTCCGGTTGA
ATGACGAAGGATGAAAAGGAAAAGACCCACGTCGACGCAATCATCGAACGCTACAAGGACCTGATGGTCGAGATTCCCCC
CGCCGACCGGCAACCCGGTCTCTCCCTGCTCTGGCCGGTTCCCGCCCAGCCCGCCATCGACAAGGGCGTCCGCCAGGCCG
AGAACTGGCTTGCCGACCAGATCGAAGGCCAGCTCTGGACCGCCTTCGCCTTCGGCCGCGACAGCCTGCCCACACCGATG
CAGAAGACCGCCTTCGAAGTGGCCTTCCTGACGCGCCTGCAGCAGCGCCTGGTAGCCGCTCGACGTTCCGGTTGA