SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0170
Regulator type	protein
Regulatory pathway (from literatures)	Global regulator
KEGG pathway (determined by BlastKOALA)	-
Related T6SS	T6SS00150 (Type i3); T6SS00152 (Type i1); T6SS00151 (Type i4a)
Strain	Pseudomonas aeruginosa UCBPP-PA14
Replicon	chromosome
Sequence	Protein sequence (109 a.a.); Nucleotide sequence (327 bp)
Description	AmrZ binds to all three assessed T6SS promoter regions and activates expression of H1-T6SS (tssA1).; AmrZ binds to all three assessed T6SS promoter regions and represses H2-T6SS(tssB2) transcription and H2-T6SS bacterial Killing.; AmrZ binds to all three assessed T6SS promoter regions and activates expression of H3-T6SS(vgrG3).
Reference	[1] Allsopp LP, Wood TE, Howard SA, et al. RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):7707-7712. doi: 10.1073/pnas.1700286114. Epub 2017 Jul 3. PMID: 28673999

External database links

Locus tag (Gene)	PA14_20290 (AmrZ)
Coordinates (Strand)	1747602..1747928 (-)
NCBI ID	489182353
RefSeq	NC_008463
Uniprot ID	V6AC97_PSEAI, A0A1C7BYS8_PSEAI, A0A1F0IH25_9PSED, A0A0H2ZEB1_PSEAB, AMRZ_PSEAE
KEGG ID	pae:PA3385, pau:PA14_20290
KEGG pathway (determined by BlastKOALA)	-
PDB ID	3QOQ

Target

Target	Regulation (↓/↑)	Target sequence
TssA1 (PA14_00990) promotor	↓	tcttttaaagatttgtccgatctgcggtgtcatgcaactagtggcgttgtgccttccttgacaagccttgcggcatgccacaagaattttgccaacctttcgagtcatccgatattcatcaatggctcctacaagatccgacggagtggatcttacaagcgaaggtgag
TssA2 (PA14_43050) promotor	↑	ccctctttgggaattttcctattcattgatacgaatcaatgaaagattttcagccaaacgtcaggcgtctgcgtccatgccctttacggcttggctatagccatcataacgacattgatggccatttggaaaaccctaccaaaacaggtagttaacaatttataaaaaataaggcagacaacagtggattcc
TssB3 (PA14_34070) promotor	↓	cctctggtccgtcactggcgacgaactacaaaagacgctagaacaggtctatggatttgcaaaataaaattttcgttggactcccatgaagcacggatgtttcaccatatgtcggaaattctccctggcgctggcgatcctggcgctggccggctgctcgtatttcttcgcgccgcgggtcgagttgcagcggctgaccctggatgtcgccgcgcgcgccaacgacgactcgccgatcgccgtggacttcgtcgcggtgaaggatgcggagctgttcaaggagctggccggactgccggccagccagtggttcgccaatcgcgagcagtaccgccgcgactaccggcagcagttcgcggtatggagcctggagctggtcccggggcagttcatcgagtcg

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
Arc	PF03869.16	1.7e-28	13..62

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-108	MRPLKQATPTYSSRTADKFVVRLPEGMREQIAEVARSHHRSMNSEIIARLEQSLLQEGALQDNLGVRLDSPELSLHERELLQRFRQLTHRQQNALVALIAHDAELAQA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9886	-	-

Protein sequence of regulator: 109 a.a. .

                                    >REG0170 NC_008463:c1747928-1747602 [Pseudomonas aeruginosa UCBPP-PA14]
MRPLKQATPTYSSRTADKFVVRLPEGMREQIAEVARSHHRSMNSEIIARLEQSLLQEGALQDNLGVRLDSPELSLHEREL
LQRFRQLTHRQQNALVALIAHDAELAQA*

                                 

Nucleotide sequence of regulator: 327 bp .

                                    >REG0170 NC_008463:c1747928-1747602 [Pseudomonas aeruginosa UCBPP-PA14]
ATGCGCCCACTGAAACAGGCAACTCCTACCTACTCCAGCCGTACCGCTGACAAATTCGTCGTTCGTCTGCCCGAGGGCAT
GCGTGAGCAGATCGCAGAAGTCGCTCGCAGCCATCACCGCAGCATGAACTCCGAGATCATCGCCCGACTCGAGCAGAGCC
TGCTCCAGGAAGGGGCGCTGCAAGACAATCTCGGTGTTCGCCTGGACAGCCCGGAACTCAGCCTGCACGAGCGCGAGCTG
CTGCAGCGTTTCCGCCAGCTGACCCACCGTCAGCAGAACGCGCTGGTCGCCCTGATCGCACACGATGCGGAGCTGGCCCA
GGCCTGA

                                