SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF00186
Effector type	-
Cognate immunity protein	-
Related T6SS (Type)	T6SS00152 (Type i1)
Strain	Pseudomonas aeruginosa UCBPP-PA14
Replicon	chromosome
Sequence	Protein sequence (173 a.a.); Nucleotide sequence (519 bp)
Description	Hcp protein
Reference	[1] Allsopp LP, Wood TE, Howard SA, et al. RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):7707-7712. doi: 10.1073/pnas.1700286114. Epub 2017 Jul 3. PMID: 28673999

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	PA14_03240 (hcp2)
Coordinates (Strand)	288454..288972 (-)
NCBI ID	116053976
RefSeq	NC_008463
Uniprot ID	UPI00000C6079
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_HCP	PF05638.14	6.5e-39	6..155

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-172	MATPAYMSITGTKQGLITAGAFTEDSVGNTYQEGHEDQVMVQGFNHEVIIPRDPQSGQPTGQRVHKPVVITKVFDKASPLLLAALTSGERLTKVEIQWYRTSAAGTQEHYYTTVLEDAIIVDIKDYMHNCQDPGNAHFTHLEDVHFTYRKITWTHEVSGTSGSDDWRSPVAG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9168	-	-

Protein sequence: 173 a.a. .

                                    >EFF00186 NC_008463:c288972-288454  (Unknown) [Pseudomonas aeruginosa UCBPP-PA14]
MATPAYMSITGTKQGLITAGAFTEDSVGNTYQEGHEDQVMVQGFNHEVIIPRDPQSGQPTGQRVHKPVVITKVFDKASPL
LLAALTSGERLTKVEIQWYRTSAAGTQEHYYTTVLEDAIIVDIKDYMHNCQDPGNAHFTHLEDVHFTYRKITWTHEVSGT
SGSDDWRSPVAG*

                                

Nucleotide sequence: 519 bp .

                                    >EFF00186 NC_008463:c288972-288454  (Unknown) [Pseudomonas aeruginosa UCBPP-PA14]
ATGGCAACCCCCGCCTATATGTCCATCACCGGCACCAAGCAGGGCCTGATCACCGCCGGCGCCTTCACCGAGGACTCGGT
CGGCAACACCTATCAGGAAGGCCACGAGGACCAGGTGATGGTCCAGGGCTTCAACCACGAAGTGATCATCCCGCGTGACC
CGCAGTCCGGCCAGCCGACCGGCCAGCGCGTGCACAAGCCTGTGGTCATCACCAAGGTCTTCGACAAGGCCTCGCCGCTG
CTGCTGGCCGCCCTGACCTCCGGCGAGCGCCTGACCAAGGTCGAGATCCAGTGGTACCGCACCTCCGCCGCCGGCACCCA
GGAGCACTACTACACCACCGTCCTGGAAGACGCGATCATCGTCGACATCAAGGACTACATGCACAACTGCCAGGACCCGG
GCAACGCCCACTTCACCCACCTGGAAGACGTGCACTTCACCTACCGCAAGATCACCTGGACCCACGAAGTCTCCGGCACC
TCCGGCTCCGACGACTGGCGCTCCCCGGTGGCAGGGTGA

                                