Detailed information of component protein


Summary

Component ID T6CP001455
Component protein type TssM
T6SS ID (Type) T6SS00150 experimental (Type i3)
Strain Pseudomonas aeruginosa UCBPP-PA14
Replicon chromosome
Sequence Protein sequence (1178 a.a.); Nucleotide sequence (3534 bp)
Reference
[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
[2] Zhang L, Hinz AJ, Nadeau JP, et al. Pseudomonas aeruginosa tssC1 links type VI secretion and biofilm-specific antibiotic resistance. J Bacteriol. 2011 Oct;193(19):5510-3. doi: 10.1128/JB.00268-11. Epub 2011 Jul 22. PMID: 21784934
[3] Lesic B, Starkey M, He J, et al. Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis. Microbiology (Reading). 2009 Sep;155(Pt 9):2845-2855. doi: 10.1099/mic.0.029082-0. Epub 2009 Jun 4. PMID: 19497948
[4] Mikkelsen H, McMullan R, Filloux A. The Pseudomonas aeruginosa reference strain PA14 displays increased virulence due to a mutation in ladS. PLoS One. 2011;6(12):e29113. doi: 10.1371/journal.pone.0029113. Epub 2011 Dec 22. PMID: 22216178
[5] Hachani A, Allsopp LP, Oduko Y, et al. The VgrG proteins are "à la carte" delivery systems for bacterial type VI effectors. J Biol Chem. 2014 Jun 20;289(25):17872-84. doi: 10.1074/jbc.M114.563429. Epub 2014 May 2. PMID: 24794869
[6] Jones C, Hachani A, Manoli E, et al. An rhs gene linked to the second type VI secretion cluster is a feature of the Pseudomonas aeruginosa strain PA14. J Bacteriol. 2014 Feb;196(4):800-10. doi: 10.1128/JB.00863-13. Epub 2013 Dec 6. PMID: 24317402
[7] Zhang L, Hinz AJ, Nadeau JP, et al. Pseudomonas aeruginosa tssC1 links type VI secretion and biofilm-specific antibiotic resistance. J Bacteriol. 2011 Oct;193(19):5510-3. doi: 10.1128/JB.00268-11. Epub 2011 Jul 22. PMID: 21784934
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PA14_RS00390
Coordinate (Strand) 89166..92699 (-)
NCBI ID WP_003111851.1
RefSeq NC_008463
Uniprot ID UPI000045D381
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 4.9e-99 195..452
IcmF-related PF06761.14 3e-86 504..797
IcmF_C PF06744.14 5.9e-28 1052..1158
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 MKGFLN
Transmembrane helix 7-29 FFARWVVPVLGLLALSLIIWFLG
Outside 30-43 PLLRLGNYEPLASA
Transmembrane helix 44-66 TSRWVLIVLLFLVWIGFRVLRIV
Inside 67-444 QARRNAAKVMQSLAEVSAPDAASVATAEELATLKQRMDEALALLKRAKLGGSERRNLYELPWYVIIGPPGSGKTTALMNSGLDFPLAAQMGAGAIRGVGGTRNCDWWFTDEAVLLDTAGRYTTQDSHAQVDKAAWLGFLDLLKTQRKRRPIDGAFIAISLSDLLLGSDAERAAHAQAIRARIQELYQQLGVRFPIYVMLTKFDLVPGFMEFFDSLNREERAQVWGMTFALDDGKSAEGPLAVFDSEFALLEQRLTARLVERLQQERDPARRDLVYGFPQQFAALRECLGEFLNGVFKPNPYEERPLLRGLYFTSGTQEGSPIDRLIGSMAQSMNLDRQHLARQTGTGRSYFIERLFREVAFGERGLVGTNPKVERRRK
Transmembrane helix 445-467 WLTIGALSATALVVLAVTAVWIA
Outside 468-1177 SYRANQSYIAAVDQRVDPLARGIESLSPAQRDVLAVLPQLNAVQNLAGDAPSWAEGYGLYQGDMLGEESASVYRKLLIAVFAPRLVTRIEEQLRSGGSSDFLYEGLKAYLMLGSPDHYDADFIKAWISLDWERNLPRDLSPEQRQALHAHLDALLERRPPSARLDQDLVEDLRRQLQQLPVAQRVYDRVKRQRLPKDVPDFRISDAAGRDAPLVFARKSGKPLTDPLSGFFTYRGYREVFLTASLSQAGTIAEEQWVLGRDLNDAGDAANLALDVRRLYFQDYLRQWDDLLADLTVVPITNVTQAADVLRILSGPTSPFRKLLEAVARETDLQKGDRLVAAQVKKAADGTVDKLKQRLGSLVGQEEEEGAREQPQQADSDPISAHFAELNSLVSKGEGGNEPAPIDSLLEDMNALYVQVSAMAGASGDSLLGDAKNQVAAAASRVALSAERQPPVVQGLVKNVVNSTTSSMMGSVRNQLNAAWISDVVSVYRQSLAGRYPIAAGSSRDATLEDFGHFFGAGGVMDSYFRQYLQPYVDTSASTWRWQPGAAQKLGINPGVLHTFQRAAAIRDAFFRSGGMQPAVRFELKPVTMDAAISQFILDLDGQQLTYDHGPARPVAMQWPSANGLGVVRLTVTPPPSSGRSGLTLEGPWAWFRLLDQSDLERGNSPDRFTLRLRIDGSSIACELRASSAFNPFKSRVVSGFSLPERL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9969 - -


  Protein sequence: 1178 a.a.    

>T6CP001455 NC_008463:c92699-89166 [Pseudomonas aeruginosa UCBPP-PA14] [TssM]
MKGFLNFFARWVVPVLGLLALSLIIWFLGPLLRLGNYEPLASATSRWVLIVLLFLVWIGFRVLRIVQARRNAAKVMQSLA
EVSAPDAASVATAEELATLKQRMDEALALLKRAKLGGSERRNLYELPWYVIIGPPGSGKTTALMNSGLDFPLAAQMGAGA
IRGVGGTRNCDWWFTDEAVLLDTAGRYTTQDSHAQVDKAAWLGFLDLLKTQRKRRPIDGAFIAISLSDLLLGSDAERAAH
AQAIRARIQELYQQLGVRFPIYVMLTKFDLVPGFMEFFDSLNREERAQVWGMTFALDDGKSAEGPLAVFDSEFALLEQRL
TARLVERLQQERDPARRDLVYGFPQQFAALRECLGEFLNGVFKPNPYEERPLLRGLYFTSGTQEGSPIDRLIGSMAQSMN
LDRQHLARQTGTGRSYFIERLFREVAFGERGLVGTNPKVERRRKWLTIGALSATALVVLAVTAVWIASYRANQSYIAAVD
QRVDPLARGIESLSPAQRDVLAVLPQLNAVQNLAGDAPSWAEGYGLYQGDMLGEESASVYRKLLIAVFAPRLVTRIEEQL
RSGGSSDFLYEGLKAYLMLGSPDHYDADFIKAWISLDWERNLPRDLSPEQRQALHAHLDALLERRPPSARLDQDLVEDLR
RQLQQLPVAQRVYDRVKRQRLPKDVPDFRISDAAGRDAPLVFARKSGKPLTDPLSGFFTYRGYREVFLTASLSQAGTIAE
EQWVLGRDLNDAGDAANLALDVRRLYFQDYLRQWDDLLADLTVVPITNVTQAADVLRILSGPTSPFRKLLEAVARETDLQ
KGDRLVAAQVKKAADGTVDKLKQRLGSLVGQEEEEGAREQPQQADSDPISAHFAELNSLVSKGEGGNEPAPIDSLLEDMN
ALYVQVSAMAGASGDSLLGDAKNQVAAAASRVALSAERQPPVVQGLVKNVVNSTTSSMMGSVRNQLNAAWISDVVSVYRQ
SLAGRYPIAAGSSRDATLEDFGHFFGAGGVMDSYFRQYLQPYVDTSASTWRWQPGAAQKLGINPGVLHTFQRAAAIRDAF
FRSGGMQPAVRFELKPVTMDAAISQFILDLDGQQLTYDHGPARPVAMQWPSANGLGVVRLTVTPPPSSGRSGLTLEGPWA
WFRLLDQSDLERGNSPDRFTLRLRIDGSSIACELRASSAFNPFKSRVVSGFSLPERL*

  Nucleotide sequence: 3534 bp    

>T6CP001455 NC_008463:c92699-89166 [Pseudomonas aeruginosa UCBPP-PA14] [TssM]
ATGAAAGGCTTCCTCAACTTCTTCGCGCGCTGGGTGGTGCCGGTGCTCGGCCTGCTCGCCCTGAGCCTGATCATCTGGTT
CCTCGGCCCGCTGCTGCGCCTGGGCAACTACGAACCGCTGGCCTCGGCGACCAGCCGCTGGGTGCTGATCGTCCTGCTGT
TCCTGGTCTGGATCGGCTTCCGCGTGCTGCGCATCGTGCAGGCCCGGCGCAACGCGGCGAAGGTCATGCAGAGTCTCGCC
GAGGTCAGCGCGCCGGACGCCGCCAGCGTCGCCACCGCCGAAGAGCTGGCGACCCTCAAGCAACGCATGGACGAGGCCCT
GGCCCTGCTCAAGCGGGCCAAGCTGGGCGGCAGCGAGCGGCGCAACCTGTACGAGTTGCCCTGGTACGTGATCATCGGCC
CGCCCGGTTCGGGCAAGACCACCGCGCTGATGAACTCCGGCCTGGACTTCCCGCTCGCCGCACAGATGGGCGCCGGGGCG
ATCCGCGGGGTCGGCGGCACGCGCAACTGCGACTGGTGGTTCACCGACGAAGCGGTGCTGCTGGACACCGCCGGCCGCTA
TACCACCCAGGACAGCCACGCCCAGGTGGACAAGGCCGCCTGGCTGGGCTTCCTCGACCTGCTCAAGACCCAGCGCAAGC
GCCGCCCCATCGATGGCGCGTTCATCGCCATCAGCCTTTCCGACCTGCTGCTGGGCAGCGACGCCGAGCGCGCCGCCCAT
GCCCAGGCGATCCGCGCGCGCATCCAGGAGCTGTACCAGCAGCTCGGGGTGCGCTTCCCGATCTACGTGATGCTGACCAA
GTTCGACCTGGTGCCGGGCTTCATGGAGTTCTTCGACAGCCTGAACCGCGAGGAACGCGCGCAGGTCTGGGGGATGACCT
TCGCCCTCGACGACGGCAAGAGCGCGGAAGGCCCGCTGGCGGTGTTCGACAGCGAATTCGCCCTGCTCGAACAGCGTCTC
ACCGCGCGCCTGGTGGAACGCCTGCAGCAGGAGCGCGACCCGGCGCGGCGCGACCTGGTCTACGGCTTCCCGCAGCAATT
CGCCGCCCTGCGCGAATGCCTCGGCGAGTTCCTCAACGGCGTGTTCAAGCCCAACCCCTACGAGGAACGCCCGCTGTTGC
GCGGCCTGTACTTCACCAGCGGAACCCAGGAAGGCAGTCCGATCGACCGCCTGATCGGCAGCATGGCGCAGTCGATGAAC
CTCGACCGCCAGCACCTGGCGCGGCAGACCGGAACCGGGCGCAGCTACTTCATCGAGCGGCTGTTCCGCGAAGTGGCGTT
CGGCGAACGCGGCCTGGTCGGCACCAACCCGAAGGTCGAGCGCCGCCGCAAATGGCTGACCATCGGCGCGCTGAGCGCCA
CCGCGCTGGTGGTGCTGGCGGTGACGGCGGTATGGATCGCCAGCTACCGCGCCAACCAGAGCTACATCGCCGCGGTCGAC
CAGCGCGTCGACCCGCTGGCGCGCGGCATCGAGTCGCTGAGCCCGGCCCAGCGCGACGTCCTGGCCGTACTGCCACAGCT
CAACGCGGTGCAGAATCTCGCCGGCGACGCGCCAAGCTGGGCCGAAGGCTACGGGCTGTACCAGGGCGACATGCTCGGCG
AGGAGTCCGCCAGCGTCTACCGCAAGCTGCTGATCGCGGTGTTCGCCCCGCGCCTGGTGACCCGCATCGAGGAACAGTTG
CGCTCCGGCGGCAGCAGCGATTTCCTCTACGAGGGCCTGAAGGCCTACCTGATGCTCGGCAGCCCGGACCATTACGACGC
CGACTTCATCAAGGCCTGGATCAGCCTCGACTGGGAACGCAACCTGCCGCGCGACCTCTCGCCGGAACAGCGCCAGGCGC
TGCACGCGCACCTCGACGCGTTGCTCGAACGACGGCCGCCGTCGGCGCGCCTGGACCAGGACCTGGTCGAGGACCTGCGC
CGCCAGTTGCAGCAACTACCGGTGGCGCAGCGGGTCTACGACCGGGTCAAGCGGCAGAGGCTGCCCAAGGACGTGCCCGA
CTTCCGCATCAGCGACGCCGCCGGCCGCGACGCACCGCTGGTGTTCGCGCGCAAGAGCGGCAAGCCGCTCACCGACCCGC
TCAGCGGCTTCTTCACCTACCGCGGCTATCGCGAGGTGTTCCTCACCGCCAGCCTCAGCCAGGCCGGCACCATCGCCGAG
GAACAGTGGGTGCTCGGGCGCGACCTGAACGATGCCGGCGATGCCGCCAACCTGGCCCTCGACGTGCGTCGCCTGTACTT
CCAGGACTACCTGCGGCAATGGGACGACCTGCTCGCCGACCTCACCGTGGTGCCGATCACCAACGTCACCCAGGCCGCCG
ACGTGCTGCGCATTCTCTCCGGGCCGACCTCGCCGTTCCGCAAGCTGCTCGAGGCGGTGGCCCGCGAGACCGACCTGCAG
AAGGGCGACCGGCTGGTCGCCGCGCAAGTGAAGAAGGCCGCCGACGGCACCGTGGACAAGCTCAAGCAGCGCCTCGGCTC
GCTGGTCGGCCAGGAGGAGGAGGAAGGCGCCCGCGAACAGCCGCAGCAGGCCGACAGCGACCCGATCAGCGCCCACTTCG
CCGAACTCAACAGCCTGGTCAGCAAGGGCGAGGGCGGCAACGAGCCGGCGCCGATCGACAGCCTGCTGGAGGACATGAAC
GCGCTCTACGTACAGGTCAGCGCCATGGCCGGAGCCAGCGGCGACTCGCTGCTCGGCGACGCCAAGAACCAGGTGGCCGC
CGCCGCCTCGCGCGTGGCCCTCAGCGCCGAGCGCCAGCCGCCGGTGGTCCAGGGCCTGGTGAAGAACGTGGTCAACTCCA
CCACCAGCAGCATGATGGGCAGCGTGCGCAACCAGCTCAACGCGGCCTGGATCAGCGACGTGGTCAGCGTCTACCGGCAG
TCCCTGGCCGGGCGCTACCCGATCGCCGCCGGCAGCTCGCGCGACGCCACCCTGGAAGACTTCGGCCACTTCTTCGGTGC
CGGTGGGGTGATGGACAGCTACTTCCGCCAGTACCTGCAACCCTACGTCGACACCTCCGCCAGCACCTGGCGCTGGCAGC
CCGGCGCGGCGCAGAAGCTCGGCATCAACCCCGGCGTGCTGCACACCTTCCAGCGCGCGGCGGCGATCCGCGACGCCTTC
TTCCGCTCCGGCGGCATGCAGCCGGCGGTGCGCTTCGAACTCAAGCCGGTGACCATGGACGCGGCGATCAGCCAGTTCAT
CCTCGACCTCGACGGCCAGCAGCTGACCTACGACCACGGCCCCGCCCGCCCGGTGGCGATGCAGTGGCCGAGCGCCAACG
GCCTCGGCGTGGTCCGCCTGACGGTGACGCCGCCGCCCAGCAGCGGCCGTTCCGGCCTGACCCTGGAGGGCCCCTGGGCC
TGGTTCCGCCTGCTCGACCAGTCGGACCTGGAACGCGGCAACTCGCCGGACCGCTTCACCCTGCGGCTGCGCATCGACGG
CTCCAGCATCGCCTGCGAGCTGCGCGCCAGCAGCGCCTTCAACCCCTTCAAGAGCCGGGTCGTGTCCGGCTTCAGCCTCC
CGGAGCGCCTTTGA