Detailed information of regulator


Summary

Regulator ID REG0164 experimental
Regulator type protein
Regulatory pathway (from literatures) QS regulatory pathway
KEGG pathway (determined by BlastKOALA) Biofilm formation - Pseudomonas aeruginosa (map02025)
Related T6SS T6SS00150 experimental (Type i3)
Strain Pseudomonas aeruginosa UCBPP-PA14
Replicon chromosome
Sequence Protein sequence (812 a.a.); Nucleotide sequence (2437 bp)
Description -
Reference
External database links

Locus tag (Gene) PA14_RS05020 (LadS)
Coordinates (Strand) 1069280..1071716 (+)
NCBI ID 15599169
RefSeq NC_008463
Uniprot ID Q9HX42_PSEAE
KEGG ID pae:PA3974
KEGG pathway (determined by BlastKOALA) map02025
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
Unknown -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
7TMR-DISM_7TM PF07695.13 2.1e-51 184..380
7TMR-DISMED2 PF07696.13 6e-37 32..171
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-182 MRHWLILFLLALPCLAGAVSFNEQVERLPLGQSIDVFEDVRGSADINDITSRAIDSSFRRHDKDVLNAGYSRSVFWLRLDLDYRPVASSDPRTWLLELAYPPLDKLDLYLPDGQGGYRLAQRTGDTLPFASRPIRQNNYLFELGLEPNKPQRVYLRLESQGSIQAPLTLWSPKAYLEEQPER
Transmembrane helix 183-205 IYVLGIIYGVLLVMLIYNLFIFL
Inside 206-211 SVRDTS
Transmembrane helix 212-234 YLYYILYIASFGLYQVSVNGAGI
Outside 235-248 EYFWPDSPWWANAA
Transmembrane helix 249-268 TPFLIGSAALFGCQFARSFL
Inside 269-279 HTRDHSVWVDR
Transmembrane helix 280-302 GLLALMAVGALVMLMALTMSYAV
Outside 303-306 ALRL
Transmembrane helix 307-329 ATYLALAFTGLIFAAGILAWLRG
Inside 330-335 MRVARY
Transmembrane helix 336-358 FIIAWTAFLLGGIVNTLMVLGYL
Outside 359-811 PNMFLTMYASQIGSALEVGLLYYVCQPDRFGAGGGLAVAGAGRPDQRDEGGARANPPGVQPQAGSAEPGTGQQQSPEGRVPRHRHPRTAHPDERGDRFAGADADGADGRRTRRVPAHRRRFGAGHDADGQRHPRPHRTAGRQALPAPRAVQPARPVRQPARAVRAARRGEGPALRPATGRQPAGHPRRRCRQAGPGAGLPGRQRDQVHRQGQRHPARRRRAYP*RGGAAGGSDRHRHRFRHGRRQRPLPAFRPGRQLVDPWLRRPRHRPGSVPQAGGAAWRRADPRVPAGTGQPLPPAPATDPAGPGPGAAAAARRRAGGAASRGVHGAGGGGQRHQPVGDSRHAAQTRLPGAHRRQRQRGPGVAGARAAGRRTARLPDAGDGRLRHLSGDPRAARLRGTAGAGVDRPQPQRRSRALPGGGHERLHGQAGQVRRVADPAARLAAVPADRHQVR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.7135 pos: 18-19. AGA-VS. MRHWLILFLLALPCLAGA


  Protein sequence of regulator: 812 a.a.    .

>REG0164 NC_008463:1069280-1071716 [Pseudomonas aeruginosa UCBPP-PA14]
MRHWLILFLLALPCLAGAVSFNEQVERLPLGQSIDVFEDVRGSADINDITSRAIDSSFRRHDKDVLNAGYSRSVFWLRLD
LDYRPVASSDPRTWLLELAYPPLDKLDLYLPDGQGGYRLAQRTGDTLPFASRPIRQNNYLFELGLEPNKPQRVYLRLESQ
GSIQAPLTLWSPKAYLEEQPERIYVLGIIYGVLLVMLIYNLFIFLSVRDTSYLYYILYIASFGLYQVSVNGAGIEYFWPD
SPWWANAATPFLIGSAALFGCQFARSFLHTRDHSVWVDRGLLALMAVGALVMLMALTMSYAVALRLATYLALAFTGLIFA
AGILAWLRGMRVARYFIIAWTAFLLGGIVNTLMVLGYLPNMFLTMYASQIGSALEVGLLYYVCQPDRFGAGGGLAVAGAG
RPDQRDEGGARANPPGVQPQAGSAEPGTGQQQSPEGRVPRHRHPRTAHPDERGDRFAGADADGADGRRTRRVPAHRRRFG
AGHDADGQRHPRPHRTAGRQALPAPRAVQPARPVRQPARAVRAARRGEGPALRPATGRQPAGHPRRRCRQAGPGAGLPGR
QRDQVHRQGQRHPARRRRAYP*RGGAAGGSDRHRHRFRHGRRQRPLPAFRPGRQLVDPWLRRPRHRPGSVPQAGGAAWRR
ADPRVPAGTGQPLPPAPATDPAGPGPGAAAAARRRAGGAASRGVHGAGGGGQRHQPVGDSRHAAQTRLPGAHRRQRQRGP
GVAGARAAGRRTARLPDAGDGRLRHLSGDPRAARLRGTAGAGVDRPQPQRRSRALPGGGHERLHGQAGQVRRVADPAARL
AAVPADRHQVRL

  Nucleotide sequence of regulator: 2437 bp    .

>REG0164 NC_008463:1069280-1071716 [Pseudomonas aeruginosa UCBPP-PA14]
ATGCGGCACTGGCTGATTCTCTTTCTTCTTGCGTTGCCGTGCCTGGCGGGTGCGGTGAGCTTCAACGAGCAGGTCGAGCG
CCTGCCGCTGGGGCAGTCCATCGACGTGTTCGAGGACGTGCGCGGTAGCGCCGATATCAACGACATCACCTCGCGGGCCA
TCGACAGCAGCTTCCGCCGCCATGACAAGGACGTGCTCAATGCCGGCTATTCGCGCTCGGTGTTCTGGTTGCGCCTGGAC
CTGGACTACCGGCCGGTCGCCTCCAGCGATCCGCGCACCTGGCTGCTGGAGCTGGCCTATCCGCCGCTGGACAAGCTCGA
CCTCTACCTGCCCGATGGCCAGGGCGGCTACCGCCTCGCCCAACGCACCGGCGACACATTGCCGTTCGCCAGTCGGCCGA
TCCGGCAGAACAACTACCTGTTCGAACTCGGCCTCGAGCCGAACAAGCCGCAGCGTGTCTACCTGCGGCTGGAAAGCCAG
GGATCGATCCAGGCGCCGTTGACCCTGTGGTCGCCCAAGGCCTACCTGGAGGAGCAGCCCGAACGCATCTATGTGCTCGG
CATCATCTATGGCGTGCTGCTGGTGATGCTGATCTACAACCTGTTCATCTTCCTCAGCGTCCGCGATACCAGCTATCTCT
ACTACATCCTCTATATCGCTTCCTTCGGGCTCTACCAGGTCTCGGTGAACGGCGCCGGCATCGAGTACTTCTGGCCTGAC
AGCCCCTGGTGGGCGAACGCCGCCACGCCATTCCTGATCGGTTCGGCGGCGCTGTTCGGCTGCCAGTTCGCGCGCAGCTT
CCTGCATACCCGCGACCACAGCGTGTGGGTCGACCGCGGCCTGCTGGCGCTGATGGCGGTCGGCGCGCTGGTCATGCTGA
TGGCGCTGACCATGAGCTACGCCGTGGCTCTACGCCTGGCCACCTACCTGGCGCTGGCCTTCACCGGCCTGATCTTCGCC
GCCGGCATCCTCGCCTGGCTGCGCGGCATGCGCGTGGCGCGCTATTTCATCATCGCCTGGACCGCCTTCCTTCTCGGCGG
CATCGTCAATACCCTGATGGTCCTCGGCTACCTGCCGAACATGTTCCTCACTATGTATGCCAGCCAGATCGGTTCGGCGC
TGGAGGTGGGCTTGCTGTACTATGTATGCCAGCCAGATCGGTTCGGCGCTGGAGGTGGGCTTGCTGTCGCTGGCGCTGGC
CGACCGGATCAACGCGATGAAGGAGGAGCGCGCGCGAATCCTCCAGGAGTCCAGCCGCAAGCTGGAAGCGCTGAACCAGG
AACTGGCCAACAGCAATCGCCTGAAGGACGAGTTCCTCGCCACCGTCACCCACGAACTGCGCACCCCGATGAGCGGGGTG
ATCGGTTCGCTGGAGCTGATGCAGACGGTGCCGATGGACGTCGAACTCGCCGAGTACCAGCGCACCGCCGCCGGTTCGGC
GCGGGACATGATGCGGATGGTCAACGACATCCTCGCCCTCATCGAACTGCAGGCCGGCAAGCTTTACCCGCGCCGCGAGC
CGTTCAGCCTGCGCGGCCTGTTCGACAGCCTGCGCGCGCAGTACGCGCCGCGCGCCGAGGAGAAGGGCCTGCGCTTCGCC
CTGCAACTGGACGACAGCCTGCCGGACACCCTCGAAGGCGATGCCGGCAAGCTGGCCCAGGCGCTGGGCTACCTGGTCGA
CAACGCGATCAAGTTCACCGCCAGGGGCAGCGTCACCCTGCGCGTCGCCGCCGGGCGTACCCATGACGGGGTGGCGCTGC
GGGTGGAAGTGATCGACACCGGCATCGGTTTCGACATGGCCGCCGGCAGCGACCTCTACCAGCGTTTCGTCCAGGCCGAC
AGCTCGTTGACCCGTGGCTACGGAGGCCTCGGCATCGGCCTGGCTCTGTGCCGCAAGCTGGTGGAGCTGCTTGGCGGCGA
GCTGACCCACGAGTCCCGGCCGGGACAGGGCAGCCGCTTCCTCCTGCGCCTGCAACTGACCCAGCCGGCCCAGGGCCTGG
CGCCGCCGCCGCGGCGCGCCGGCGGGCAGGCGGTGCGGCGTCCCGAGGAGTGCACGGTGCTGGTGGTGGAGGACAACGCC
ATCAACCAGTTGGTGACTCGCGGCATGCTGCTCAAACTCGGCTACCGGGTGCGCACCGCCGACAACGGCAGCGAGGCCCT
GGAGTTGCTGGCGCGCGAGCGGCCGGACGGCGTACTGCTCGACTGCCAGATGCCGGTGATGGACGGCTTCGCCACCTGTC
GGGCGATCCGCGCGCTGCCCGGCTGCGCGGAACTGCCGGTGCTGGCGTTGACCGCCCACAGCCACAGCGGCGATCGCGAG
CGCTGCCTGGCGGCGGGCATGAGCGACTACATGGCCAAGCCGGTCAAGTTCGAAGAGTTGCAGACCCTGCTGCACGATTG
GCTGCTGTGCCAGCCGATCGTCACCAAGTCCGCCTGA