Detailed information of regulator


Summary

Regulator ID REG0029 experimental
Regulator type protein
Regulatory pathway (from literatures) QS regulatory pathway
KEGG pathway (determined by BlastKOALA) Two-component system (map02020)
Related T6SS T6SS01168 experimental (Type i1)
Strain Vibrio cholerae C6706
Replicon chromosome
Sequence Protein sequence (451 a.a.); Nucleotide sequence (1353 bp)
Description In the absence of the Qrr sRNAs, LuxO∼P cannot control T6SS shows that LuxO functions through the Qrr sRNAs to repress T6SS. Deletion of luxO or tsrA alone each caused a small but significant increase in transcription of hcp1 and the T6SS structural gene VCA0107, and simultaneous deletion of both genes (ΔluxO tsrA) caused a further increase in their expression. Phosphorylated LuxO (LuxO~P) indirectly (via the regulation of small RNAs) leads to the degradation of hapR mRNA.
Reference
External database links

Locus tag (Gene) GPY04_RS15835 (luxO)
Coordinates (Strand) 273631..274983 (-)
NCBI ID 446367907
RefSeq NZ_CP046845
Uniprot ID Q9KN24_VIBCH, A0A0X1L1S6_VIBCO, A0A0H3AF68_VIBC3, A0A0K9UXM7_VIBCL, A0A0H3Q3M0_VIBCL, C3LUG6_VIBCM, A0A0H7FVS1_VIBCL
KEGG ID vcq:EN18_04425, vcm:VCM66_A0140, vco:VC0395_1135, vch:VCA0142
KEGG pathway (determined by BlastKOALA) map02020
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
VC0070 (tsrA) ↑ -
GPY04_RS00870 (hapR) ↑ 446262243
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Sigma54_activat PF00158.28 3.2e-64 145..308
Response_reg PF00072.26 1.9e-26 6..115
HTH_8 PF02954.21 2e-10 400..438
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-450 VESQRIALIEDDDIVRQATGQWLQLAGFDVAMFADGASALAAIEQQSFAAVVSDVRLPDTDGLSLLVALKNVQSLMPVILITGHGDVDMAVKALHQGAFDFIEKPFQPERLANTVAQAVSQYQNALSSQSRAHYLASAKGLEQILIGQCKSIQLLREQIAKVAAMDTNVIIYGETGCGKELVAQCLHQASERQRGQFVAINCGAIPENLFESELFGHEAGAFTGAVKRRIGKLELADKGTLFLDEIESMPLAMQVKVLRVLQDHVVERVGSNQPITIDLRVIAAAKEELRQHAEFRQDLFYRLNVAQLYLPPLRERGDDILLLFEHFCVQVKPTWRSLSEADRLALLTYRWPGNVRELRNVALRYALDDSISVGEILASCPGMTEEEASAGLPLAIQVHNFERKVIAQALHRHQGNISEVMKELDLPRRTLNQKMQKFGLLRGDFTAQEE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9648 - -


  Protein sequence of regulator: 451 a.a.    .

>REG0029 NZ_CP046845:c274983-273631 [Vibrio cholerae C6706]
VESQRIALIEDDDIVRQATGQWLQLAGFDVAMFADGASALAAIEQQSFAAVVSDVRLPDTDGLSLLVALKNVQSLMPVIL
ITGHGDVDMAVKALHQGAFDFIEKPFQPERLANTVAQAVSQYQNALSSQSRAHYLASAKGLEQILIGQCKSIQLLREQIA
KVAAMDTNVIIYGETGCGKELVAQCLHQASERQRGQFVAINCGAIPENLFESELFGHEAGAFTGAVKRRIGKLELADKGT
LFLDEIESMPLAMQVKVLRVLQDHVVERVGSNQPITIDLRVIAAAKEELRQHAEFRQDLFYRLNVAQLYLPPLRERGDDI
LLLFEHFCVQVKPTWRSLSEADRLALLTYRWPGNVRELRNVALRYALDDSISVGEILASCPGMTEEEASAGLPLAIQVHN
FERKVIAQALHRHQGNISEVMKELDLPRRTLNQKMQKFGLLRGDFTAQEE*

  Nucleotide sequence of regulator: 1353 bp    .

>REG0029 NZ_CP046845:c274983-273631 [Vibrio cholerae C6706]
GTGGAAAGCCAACGGATTGCATTGATTGAGGACGACGACATTGTGCGTCAAGCCACTGGCCAGTGGTTACAACTGGCGGG
TTTTGACGTGGCGATGTTTGCTGATGGCGCATCCGCGTTAGCGGCGATTGAGCAGCAGAGTTTCGCCGCCGTGGTGAGTG
ATGTACGCCTGCCCGATACCGATGGATTGAGTCTGTTGGTGGCGTTGAAAAATGTGCAATCGCTGATGCCAGTGATTTTG
ATCACTGGGCATGGCGATGTCGACATGGCGGTGAAAGCACTGCATCAAGGCGCGTTCGATTTTATCGAGAAGCCATTCCA
ACCGGAGCGCCTTGCCAACACCGTGGCGCAAGCGGTCAGTCAGTACCAAAACGCGCTGTCATCGCAGTCGCGTGCCCACT
ATCTTGCCTCTGCCAAAGGGTTAGAGCAGATCTTGATTGGGCAGTGTAAGTCGATTCAACTGCTGCGCGAGCAAATCGCC
AAAGTCGCAGCGATGGATACCAACGTGATCATTTACGGCGAAACTGGCTGTGGCAAAGAACTGGTGGCGCAGTGTCTGCA
TCAAGCCAGCGAGCGTCAGCGTGGTCAGTTTGTGGCGATCAACTGTGGCGCGATCCCAGAAAATCTGTTTGAAAGTGAAC
TGTTCGGCCATGAAGCGGGCGCGTTTACCGGTGCGGTGAAGCGGAGGATTGGCAAGCTGGAACTGGCCGATAAAGGCACG
CTGTTTCTGGATGAAATCGAGAGCATGCCGCTGGCCATGCAAGTGAAAGTGCTCCGCGTGCTGCAAGATCATGTGGTAGA
GCGGGTGGGCAGTAATCAGCCGATCACCATTGATCTGCGGGTCATCGCGGCAGCCAAGGAAGAGCTGCGTCAACACGCGG
AATTTCGCCAAGATCTCTTTTACCGCCTTAATGTCGCGCAGCTCTATTTACCGCCGCTGCGTGAGCGAGGCGACGATATT
TTGCTGCTGTTTGAACACTTCTGTGTACAAGTGAAACCGACTTGGCGCAGCCTGAGTGAAGCGGATCGGTTGGCGCTGCT
TACTTATCGCTGGCCGGGCAACGTGCGCGAGCTGCGCAATGTCGCGCTGCGTTATGCGCTGGATGACAGCATCAGTGTTG
GGGAAATTCTGGCTTCATGTCCGGGAATGACTGAAGAAGAAGCTTCAGCAGGGTTACCGCTGGCGATTCAAGTGCACAAC
TTTGAACGTAAAGTGATCGCTCAGGCGCTGCATCGCCATCAAGGTAACATCAGTGAAGTGATGAAAGAGCTGGATTTGCC
AAGGCGCACCCTAAATCAGAAAATGCAGAAGTTTGGTTTGCTTCGCGGTGATTTTACTGCGCAGGAGGAATGA