Detailed information of regulator
Summary
| Regulator ID | REG0355  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | QS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | Two-component system (map02020); Quorum sensing (map02024); Biofilm formation - Vibrio cholerae (map05111) |
| Related T6SS | T6SS01280 (Type i5); T6SS01281 (Type i1) |
| Strain | Vibrio coralliilyticus OCN008 |
| Replicon | chromosome |
| Sequence | Protein sequence (202 a.a.); Nucleotide sequence (606 bp) |
| Description | The ΔvtpR mutant recovery was reduced by nearly one log-fold in the challenge against T6SS+ V.cholerae in comparison to the challenge against T6SS- V.cholerae and that it was unable to kill V.cholerae shows VtpR did play a role in the success of V.coralliilyticus survival against T6SS+ V.cholerae. |
| Reference |
External database links
| Locus tag (Gene) | N779_15250 (VtpR) |
| Coordinates (Strand) | 7579..8184 (-) |
| NCBI ID | 536747482 |
| GenBank | AVOO01000045 |
| Uniprot ID | UPI0001B949AA |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | map02020; map02024; map05111 |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| Unknown | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| TetR_N | PF00440.25 | 5.8e-09 | 22..66 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-201 | MDSIAKRPRTRLSPQKRKQQLMEIALEVFARRGIGRGGHADIAEIAQVSVATVFNYFPTREDLVDDVLNYVVRQFSNFLSDNIDLDIHAKENLRNLTSAMVDLVVNDCHWLKVWFEWSASTRDEVWPLFVTTNRTNQLLVQNMFIKAIERGEVCDDHDPADLATLFHGICYSLFVQANRTQDDESVNRLTQSYLDMLCIYK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9899 | - | - |
Protein sequence of regulator: 202 a.a. .
>REG0355 AVOO01000045:c8184-7579 [Vibrio coralliilyticus OCN008]
MDSIAKRPRTRLSPQKRKQQLMEIALEVFARRGIGRGGHADIAEIAQVSVATVFNYFPTREDLVDDVLNYVVRQFSNFLS
DNIDLDIHAKENLRNLTSAMVDLVVNDCHWLKVWFEWSASTRDEVWPLFVTTNRTNQLLVQNMFIKAIERGEVCDDHDPA
DLATLFHGICYSLFVQANRTQDDESVNRLTQSYLDMLCIYK*
MDSIAKRPRTRLSPQKRKQQLMEIALEVFARRGIGRGGHADIAEIAQVSVATVFNYFPTREDLVDDVLNYVVRQFSNFLS
DNIDLDIHAKENLRNLTSAMVDLVVNDCHWLKVWFEWSASTRDEVWPLFVTTNRTNQLLVQNMFIKAIERGEVCDDHDPA
DLATLFHGICYSLFVQANRTQDDESVNRLTQSYLDMLCIYK*
Nucleotide sequence of regulator: 606 bp .
>REG0355 AVOO01000045:c8184-7579 [Vibrio coralliilyticus OCN008]
ATGGATTCTATAGCTAAGAGACCGCGTACGCGTCTTTCACCACAAAAACGCAAACAACAACTGATGGAAATCGCATTAGA
GGTGTTTGCTCGTCGTGGTATTGGTCGCGGTGGTCATGCTGACATTGCAGAAATCGCTCAGGTTTCTGTTGCTACTGTTT
TTAACTACTTCCCAACCCGCGAAGACTTGGTTGATGATGTACTTAACTACGTAGTTCGTCAGTTCTCAAACTTCCTATCA
GATAATATCGATCTTGATATTCATGCAAAAGAAAACCTACGCAACCTAACTTCGGCAATGGTTGACCTTGTTGTCAACGA
CTGCCACTGGCTTAAAGTTTGGTTCGAGTGGAGTGCATCGACACGTGATGAAGTATGGCCACTGTTTGTTACGACAAACC
GTACCAACCAGCTTCTAGTTCAGAACATGTTTATCAAAGCAATCGAGCGCGGTGAAGTATGTGATGATCACGACCCAGCA
GACCTAGCGACACTATTCCACGGCATCTGCTACTCATTGTTTGTCCAGGCTAACCGTACTCAAGATGATGAGTCTGTAAA
CCGCCTGACACAAAGCTACCTAGATATGCTTTGCATCTACAAGTAG
ATGGATTCTATAGCTAAGAGACCGCGTACGCGTCTTTCACCACAAAAACGCAAACAACAACTGATGGAAATCGCATTAGA
GGTGTTTGCTCGTCGTGGTATTGGTCGCGGTGGTCATGCTGACATTGCAGAAATCGCTCAGGTTTCTGTTGCTACTGTTT
TTAACTACTTCCCAACCCGCGAAGACTTGGTTGATGATGTACTTAACTACGTAGTTCGTCAGTTCTCAAACTTCCTATCA
GATAATATCGATCTTGATATTCATGCAAAAGAAAACCTACGCAACCTAACTTCGGCAATGGTTGACCTTGTTGTCAACGA
CTGCCACTGGCTTAAAGTTTGGTTCGAGTGGAGTGCATCGACACGTGATGAAGTATGGCCACTGTTTGTTACGACAAACC
GTACCAACCAGCTTCTAGTTCAGAACATGTTTATCAAAGCAATCGAGCGCGGTGAAGTATGTGATGATCACGACCCAGCA
GACCTAGCGACACTATTCCACGGCATCTGCTACTCATTGTTTGTCCAGGCTAACCGTACTCAAGATGATGAGTCTGTAAA
CCGCCTGACACAAAGCTACCTAGATATGCTTTGCATCTACAAGTAG