Detailed information of regulator


Summary

Regulator ID REG0353 experimental
Regulator type protein
Regulatory pathway (from literatures) QS regulatory pathway
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00157 experimental (Type i1)
Strain Aeromonas hydrophila subsp. hydrophila ATCC 7966
Replicon chromosome
Sequence Protein sequence (513 a.a.); Nucleotide sequence (1539 bp)
Description The reporter fusion assays results that the bEBPs bind to the T6SS gene cluster promoters to activate their transcription and EMSA assays results that bEBPs specifically bind the promoter region of their cognate T6SS gene cluster.
Reference

External database links

Locus tag (Gene) AHA_1842 (VasH)
Coordinates (Strand) 2010521..2012059 (+)
NCBI ID 117617496
RefSeq NC_008570
Uniprot ID A0KJC5_AERHH
KEGG ID aha:AHA_1842
KEGG pathway (determined by BlastKOALA) -
PDB ID -

  Target
Target Regulation (↓/↑) Target sequence
T6SS gene cluster promoter (PAHA1826) gcgaagggcagggaacatagagcccaagctatctcttcagtgacagccatagattgccccgcgaaggcacactatccaagtaaccggcccgatggtcggtcacttggatatatgggtgatgctgtgtttggcgtctgggcgttatgcaaggaagcttgccaagctgtcccatctcattgagcagtgcgcccaccacgttgcctctcttcttacttattacgctggtgccaagagacctctttttcgaagacatcttggcgcgcttctttctgtttctctctgccaatggtgagagtctttggtcgcccacgctttgattggccaagtgcctatcttcagtaagtgatcgccgggtaacaggtgaggggtagatataaactcttccctgcgcaatgaattgccatttattcagtgattgatgctggttgtgcaaggtcgtgtagttttatgtgccacctacgcaacttgttgcgccatatggcggctgtgcaagtgagggggcgttttaattatttgttttttaacaaataattagtttggcatggtcactgctaacggattcggtcgagtgtcattaccgttcaaccaatcaggagcatacccatg

Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Sigma54_activat PF00158.28 8.8e-66 198..361

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-512 MEQALAFSLALTAQRDEPHLCHWWSSTVHASFQPRGLLLGMLDVSGRQLECQGWVRGKEVALGLAVDDFSHPLAYVLHKAQSRTWDSLYGGARIEHAGFRALLANLGQQCGLHAYPLLDGNGKPFAVLALMDEGEVLRAWADGPELAQLSQVFCNQLTLIRDLGRSRRDQGVLRDSLRQMKGEGERLRQHEKLLGDQLVGQSAVIRGLREQINQAGQHKLTVLIQGETGSGKEVVARLVHQCSDRANKPFVAINCAAIPENLIESELFGYQKGAFSGALANKTGLVAQANGGTLFLDEVGDMPAAMQAKLLRVLETRSYRPLGAEQEYHSDFRLIAATHQPLTRHVEEGQFRADLYHRLCQCLLLIAPLREHMDDVPLLCQHFMAQFAAQDGKTLVGLQRKFLKQLQTYDFPGNVRELRNLLEVACAHTRHGEEVGLEALPPELRERVSALLPSSMDDYNHIRDLRRAMQQYEASVIEARLRHFQGNRMLVAESLNIPKRTLDHKCQKLEVN



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9541 - -



  Protein sequence of regulator: 513 a.a.    .

>REG0353 NC_008570:2010521-2012059 [Aeromonas hydrophila subsp. hydrophila ATCC 7966]
MEQALAFSLALTAQRDEPHLCHWWSSTVHASFQPRGLLLGMLDVSGRQLECQGWVRGKEVALGLAVDDFSHPLAYVLHKA
QSRTWDSLYGGARIEHAGFRALLANLGQQCGLHAYPLLDGNGKPFAVLALMDEGEVLRAWADGPELAQLSQVFCNQLTLI
RDLGRSRRDQGVLRDSLRQMKGEGERLRQHEKLLGDQLVGQSAVIRGLREQINQAGQHKLTVLIQGETGSGKEVVARLVH
QCSDRANKPFVAINCAAIPENLIESELFGYQKGAFSGALANKTGLVAQANGGTLFLDEVGDMPAAMQAKLLRVLETRSYR
PLGAEQEYHSDFRLIAATHQPLTRHVEEGQFRADLYHRLCQCLLLIAPLREHMDDVPLLCQHFMAQFAAQDGKTLVGLQR
KFLKQLQTYDFPGNVRELRNLLEVACAHTRHGEEVGLEALPPELRERVSALLPSSMDDYNHIRDLRRAMQQYEASVIEAR
LRHFQGNRMLVAESLNIPKRTLDHKCQKLEVN*

  Nucleotide sequence of regulator: 1539 bp    .

>REG0353 NC_008570:2010521-2012059 [Aeromonas hydrophila subsp. hydrophila ATCC 7966]
ATGGAGCAAGCCCTCGCATTTTCCCTGGCCCTCACCGCCCAGCGCGATGAGCCACACCTGTGCCACTGGTGGAGCTCGAC
CGTCCATGCCAGCTTCCAGCCCAGGGGGCTGCTGCTCGGCATGCTGGATGTGAGCGGCCGCCAGCTGGAGTGCCAAGGCT
GGGTCAGGGGGAAGGAGGTGGCGCTGGGGCTGGCGGTGGACGATTTTTCCCACCCCCTTGCCTATGTGCTGCACAAGGCC
CAGTCCCGTACCTGGGACTCCCTCTACGGCGGTGCCCGCATCGAGCACGCCGGTTTTCGCGCACTGCTGGCCAACCTTGG
CCAGCAGTGCGGGCTGCACGCGTATCCGCTGCTGGATGGCAACGGCAAGCCGTTTGCCGTGCTGGCCCTGATGGATGAAG
GGGAGGTGCTGCGGGCCTGGGCCGACGGCCCCGAGCTGGCCCAGCTCTCCCAGGTCTTTTGCAACCAGCTCACCCTCATT
CGCGATCTCGGTCGCAGTCGGCGGGATCAGGGGGTACTGCGCGACTCCCTGCGCCAGATGAAGGGGGAGGGGGAGCGGCT
GCGCCAGCACGAGAAGCTGCTCGGCGATCAGCTGGTCGGCCAGTCGGCGGTGATCCGCGGGCTGCGCGAGCAGATCAACC
AGGCCGGCCAGCACAAGCTGACCGTGCTGATCCAGGGGGAGACCGGCAGCGGCAAGGAGGTGGTGGCACGGCTGGTGCAC
CAGTGCTCCGATCGCGCCAACAAGCCGTTTGTCGCCATCAACTGCGCCGCCATCCCGGAGAACCTCATCGAGAGCGAGCT
GTTTGGTTACCAGAAGGGAGCCTTCTCCGGCGCCCTTGCCAACAAGACCGGGCTGGTGGCCCAGGCCAACGGTGGCACCC
TGTTTCTGGACGAGGTGGGGGACATGCCCGCTGCCATGCAGGCCAAGCTGCTGCGGGTGCTGGAAACCCGCAGTTACCGG
CCGCTCGGGGCCGAGCAGGAGTATCACTCCGATTTTCGCCTCATCGCCGCTACCCATCAGCCGCTCACCCGACATGTGGA
GGAGGGGCAGTTTAGGGCCGACCTCTACCACCGTCTCTGTCAGTGCCTGCTGCTGATTGCCCCCCTGCGCGAACACATGG
ATGACGTGCCGCTGTTGTGCCAGCACTTCATGGCCCAGTTTGCTGCCCAAGATGGCAAGACCTTGGTGGGGCTGCAGCGC
AAGTTCTTGAAACAGCTGCAGACCTATGACTTCCCCGGCAACGTGCGCGAGCTGCGCAACCTGCTGGAGGTGGCCTGCGC
CCATACCCGTCACGGGGAGGAGGTGGGGCTGGAGGCGCTGCCGCCGGAGCTGCGCGAGCGGGTCAGCGCCCTGCTGCCCT
CTTCCATGGACGACTACAACCACATCCGCGATCTGCGCCGGGCCATGCAGCAGTATGAGGCTTCCGTCATCGAGGCGCGG
CTGCGCCACTTCCAGGGCAATCGGATGCTGGTGGCCGAGAGCCTCAACATTCCCAAGCGCACCCTGGATCACAAGTGCCA
GAAACTGGAGGTGAACTGA