Detailed information of regulator
Summary
| Regulator ID | REG0353  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | QS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00157 (Type i1) |
| Strain | Aeromonas hydrophila subsp. hydrophila ATCC 7966 |
| Replicon | chromosome |
| Sequence | Protein sequence (513 a.a.); Nucleotide sequence (1539 bp) |
| Description | The reporter fusion assays results that the bEBPs bind to the T6SS gene cluster promoters to activate their transcription and EMSA assays results that bEBPs specifically bind the promoter region of their cognate T6SS gene cluster. |
| Reference |
External database links
| Locus tag (Gene) | AHA_1842 (VasH) |
| Coordinates (Strand) | 2010521..2012059 (+) |
| NCBI ID | 117617496 |
| RefSeq | NC_008570 |
| Uniprot ID | A0KJC5_AERHH |
| KEGG ID | aha:AHA_1842 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| T6SS gene cluster promoter (PAHA1826) | ↓ | gcgaagggcagggaacatagagcccaagctatctcttcagtgacagccatagattgccccgcgaaggcacactatccaagtaaccggcccgatggtcggtcacttggatatatgggtgatgctgtgtttggcgtctgggcgttatgcaaggaagcttgccaagctgtcccatctcattgagcagtgcgcccaccacgttgcctctcttcttacttattacgctggtgccaagagacctctttttcgaagacatcttggcgcgcttctttctgtttctctctgccaatggtgagagtctttggtcgcccacgctttgattggccaagtgcctatcttcagtaagtgatcgccgggtaacaggtgaggggtagatataaactcttccctgcgcaatgaattgccatttattcagtgattgatgctggttgtgcaaggtcgtgtagttttatgtgccacctacgcaacttgttgcgccatatggcggctgtgcaagtgagggggcgttttaattatttgttttttaacaaataattagtttggcatggtcactgctaacggattcggtcgagtgtcattaccgttcaaccaatcaggagcatacccatg |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Sigma54_activat | PF00158.28 | 8.8e-66 | 198..361 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-512 | MEQALAFSLALTAQRDEPHLCHWWSSTVHASFQPRGLLLGMLDVSGRQLECQGWVRGKEVALGLAVDDFSHPLAYVLHKAQSRTWDSLYGGARIEHAGFRALLANLGQQCGLHAYPLLDGNGKPFAVLALMDEGEVLRAWADGPELAQLSQVFCNQLTLIRDLGRSRRDQGVLRDSLRQMKGEGERLRQHEKLLGDQLVGQSAVIRGLREQINQAGQHKLTVLIQGETGSGKEVVARLVHQCSDRANKPFVAINCAAIPENLIESELFGYQKGAFSGALANKTGLVAQANGGTLFLDEVGDMPAAMQAKLLRVLETRSYRPLGAEQEYHSDFRLIAATHQPLTRHVEEGQFRADLYHRLCQCLLLIAPLREHMDDVPLLCQHFMAQFAAQDGKTLVGLQRKFLKQLQTYDFPGNVRELRNLLEVACAHTRHGEEVGLEALPPELRERVSALLPSSMDDYNHIRDLRRAMQQYEASVIEARLRHFQGNRMLVAESLNIPKRTLDHKCQKLEVN |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9541 | - | - |
Protein sequence of regulator: 513 a.a. .
>REG0353 NC_008570:2010521-2012059 [Aeromonas hydrophila subsp. hydrophila ATCC 7966]
MEQALAFSLALTAQRDEPHLCHWWSSTVHASFQPRGLLLGMLDVSGRQLECQGWVRGKEVALGLAVDDFSHPLAYVLHKA
QSRTWDSLYGGARIEHAGFRALLANLGQQCGLHAYPLLDGNGKPFAVLALMDEGEVLRAWADGPELAQLSQVFCNQLTLI
RDLGRSRRDQGVLRDSLRQMKGEGERLRQHEKLLGDQLVGQSAVIRGLREQINQAGQHKLTVLIQGETGSGKEVVARLVH
QCSDRANKPFVAINCAAIPENLIESELFGYQKGAFSGALANKTGLVAQANGGTLFLDEVGDMPAAMQAKLLRVLETRSYR
PLGAEQEYHSDFRLIAATHQPLTRHVEEGQFRADLYHRLCQCLLLIAPLREHMDDVPLLCQHFMAQFAAQDGKTLVGLQR
KFLKQLQTYDFPGNVRELRNLLEVACAHTRHGEEVGLEALPPELRERVSALLPSSMDDYNHIRDLRRAMQQYEASVIEAR
LRHFQGNRMLVAESLNIPKRTLDHKCQKLEVN*
MEQALAFSLALTAQRDEPHLCHWWSSTVHASFQPRGLLLGMLDVSGRQLECQGWVRGKEVALGLAVDDFSHPLAYVLHKA
QSRTWDSLYGGARIEHAGFRALLANLGQQCGLHAYPLLDGNGKPFAVLALMDEGEVLRAWADGPELAQLSQVFCNQLTLI
RDLGRSRRDQGVLRDSLRQMKGEGERLRQHEKLLGDQLVGQSAVIRGLREQINQAGQHKLTVLIQGETGSGKEVVARLVH
QCSDRANKPFVAINCAAIPENLIESELFGYQKGAFSGALANKTGLVAQANGGTLFLDEVGDMPAAMQAKLLRVLETRSYR
PLGAEQEYHSDFRLIAATHQPLTRHVEEGQFRADLYHRLCQCLLLIAPLREHMDDVPLLCQHFMAQFAAQDGKTLVGLQR
KFLKQLQTYDFPGNVRELRNLLEVACAHTRHGEEVGLEALPPELRERVSALLPSSMDDYNHIRDLRRAMQQYEASVIEAR
LRHFQGNRMLVAESLNIPKRTLDHKCQKLEVN*
Nucleotide sequence of regulator: 1539 bp .
>REG0353 NC_008570:2010521-2012059 [Aeromonas hydrophila subsp. hydrophila ATCC 7966]
ATGGAGCAAGCCCTCGCATTTTCCCTGGCCCTCACCGCCCAGCGCGATGAGCCACACCTGTGCCACTGGTGGAGCTCGAC
CGTCCATGCCAGCTTCCAGCCCAGGGGGCTGCTGCTCGGCATGCTGGATGTGAGCGGCCGCCAGCTGGAGTGCCAAGGCT
GGGTCAGGGGGAAGGAGGTGGCGCTGGGGCTGGCGGTGGACGATTTTTCCCACCCCCTTGCCTATGTGCTGCACAAGGCC
CAGTCCCGTACCTGGGACTCCCTCTACGGCGGTGCCCGCATCGAGCACGCCGGTTTTCGCGCACTGCTGGCCAACCTTGG
CCAGCAGTGCGGGCTGCACGCGTATCCGCTGCTGGATGGCAACGGCAAGCCGTTTGCCGTGCTGGCCCTGATGGATGAAG
GGGAGGTGCTGCGGGCCTGGGCCGACGGCCCCGAGCTGGCCCAGCTCTCCCAGGTCTTTTGCAACCAGCTCACCCTCATT
CGCGATCTCGGTCGCAGTCGGCGGGATCAGGGGGTACTGCGCGACTCCCTGCGCCAGATGAAGGGGGAGGGGGAGCGGCT
GCGCCAGCACGAGAAGCTGCTCGGCGATCAGCTGGTCGGCCAGTCGGCGGTGATCCGCGGGCTGCGCGAGCAGATCAACC
AGGCCGGCCAGCACAAGCTGACCGTGCTGATCCAGGGGGAGACCGGCAGCGGCAAGGAGGTGGTGGCACGGCTGGTGCAC
CAGTGCTCCGATCGCGCCAACAAGCCGTTTGTCGCCATCAACTGCGCCGCCATCCCGGAGAACCTCATCGAGAGCGAGCT
GTTTGGTTACCAGAAGGGAGCCTTCTCCGGCGCCCTTGCCAACAAGACCGGGCTGGTGGCCCAGGCCAACGGTGGCACCC
TGTTTCTGGACGAGGTGGGGGACATGCCCGCTGCCATGCAGGCCAAGCTGCTGCGGGTGCTGGAAACCCGCAGTTACCGG
CCGCTCGGGGCCGAGCAGGAGTATCACTCCGATTTTCGCCTCATCGCCGCTACCCATCAGCCGCTCACCCGACATGTGGA
GGAGGGGCAGTTTAGGGCCGACCTCTACCACCGTCTCTGTCAGTGCCTGCTGCTGATTGCCCCCCTGCGCGAACACATGG
ATGACGTGCCGCTGTTGTGCCAGCACTTCATGGCCCAGTTTGCTGCCCAAGATGGCAAGACCTTGGTGGGGCTGCAGCGC
AAGTTCTTGAAACAGCTGCAGACCTATGACTTCCCCGGCAACGTGCGCGAGCTGCGCAACCTGCTGGAGGTGGCCTGCGC
CCATACCCGTCACGGGGAGGAGGTGGGGCTGGAGGCGCTGCCGCCGGAGCTGCGCGAGCGGGTCAGCGCCCTGCTGCCCT
CTTCCATGGACGACTACAACCACATCCGCGATCTGCGCCGGGCCATGCAGCAGTATGAGGCTTCCGTCATCGAGGCGCGG
CTGCGCCACTTCCAGGGCAATCGGATGCTGGTGGCCGAGAGCCTCAACATTCCCAAGCGCACCCTGGATCACAAGTGCCA
GAAACTGGAGGTGAACTGA
ATGGAGCAAGCCCTCGCATTTTCCCTGGCCCTCACCGCCCAGCGCGATGAGCCACACCTGTGCCACTGGTGGAGCTCGAC
CGTCCATGCCAGCTTCCAGCCCAGGGGGCTGCTGCTCGGCATGCTGGATGTGAGCGGCCGCCAGCTGGAGTGCCAAGGCT
GGGTCAGGGGGAAGGAGGTGGCGCTGGGGCTGGCGGTGGACGATTTTTCCCACCCCCTTGCCTATGTGCTGCACAAGGCC
CAGTCCCGTACCTGGGACTCCCTCTACGGCGGTGCCCGCATCGAGCACGCCGGTTTTCGCGCACTGCTGGCCAACCTTGG
CCAGCAGTGCGGGCTGCACGCGTATCCGCTGCTGGATGGCAACGGCAAGCCGTTTGCCGTGCTGGCCCTGATGGATGAAG
GGGAGGTGCTGCGGGCCTGGGCCGACGGCCCCGAGCTGGCCCAGCTCTCCCAGGTCTTTTGCAACCAGCTCACCCTCATT
CGCGATCTCGGTCGCAGTCGGCGGGATCAGGGGGTACTGCGCGACTCCCTGCGCCAGATGAAGGGGGAGGGGGAGCGGCT
GCGCCAGCACGAGAAGCTGCTCGGCGATCAGCTGGTCGGCCAGTCGGCGGTGATCCGCGGGCTGCGCGAGCAGATCAACC
AGGCCGGCCAGCACAAGCTGACCGTGCTGATCCAGGGGGAGACCGGCAGCGGCAAGGAGGTGGTGGCACGGCTGGTGCAC
CAGTGCTCCGATCGCGCCAACAAGCCGTTTGTCGCCATCAACTGCGCCGCCATCCCGGAGAACCTCATCGAGAGCGAGCT
GTTTGGTTACCAGAAGGGAGCCTTCTCCGGCGCCCTTGCCAACAAGACCGGGCTGGTGGCCCAGGCCAACGGTGGCACCC
TGTTTCTGGACGAGGTGGGGGACATGCCCGCTGCCATGCAGGCCAAGCTGCTGCGGGTGCTGGAAACCCGCAGTTACCGG
CCGCTCGGGGCCGAGCAGGAGTATCACTCCGATTTTCGCCTCATCGCCGCTACCCATCAGCCGCTCACCCGACATGTGGA
GGAGGGGCAGTTTAGGGCCGACCTCTACCACCGTCTCTGTCAGTGCCTGCTGCTGATTGCCCCCCTGCGCGAACACATGG
ATGACGTGCCGCTGTTGTGCCAGCACTTCATGGCCCAGTTTGCTGCCCAAGATGGCAAGACCTTGGTGGGGCTGCAGCGC
AAGTTCTTGAAACAGCTGCAGACCTATGACTTCCCCGGCAACGTGCGCGAGCTGCGCAACCTGCTGGAGGTGGCCTGCGC
CCATACCCGTCACGGGGAGGAGGTGGGGCTGGAGGCGCTGCCGCCGGAGCTGCGCGAGCGGGTCAGCGCCCTGCTGCCCT
CTTCCATGGACGACTACAACCACATCCGCGATCTGCGCCGGGCCATGCAGCAGTATGAGGCTTCCGTCATCGAGGCGCGG
CTGCGCCACTTCCAGGGCAATCGGATGCTGGTGGCCGAGAGCCTCAACATTCCCAAGCGCACCCTGGATCACAAGTGCCA
GAAACTGGAGGTGAACTGA