Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00157 experimental
T6SS type Type i1
Strain Aeromonas hydrophila subsp. hydrophila ATCC 7966
Replicon (RefSeq) chromosome (NC_008570)
Location 1984799..2036268
Function -
Comment This T6SS can secrete HCP.
References
[1] Wang N et al (2015) Protective efficacy of recombinant hemolysin co-regulated protein (Hcp) of Aeromonas hydrophila in common carp (Cyprinus carpio). Fish Shellfish Immunol. 46(2):297-304. [PudMed:26093203]
[2] Suarez G et al (2010) A type VI secretion system effector protein, VgrG1, from Aeromonas hydrophila that induces host cell toxicity by ADP ribosylation of actin. J Bacteriol. 192(1):155-68. [PudMed:19880608]
[3] Sha J et al (2013) Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis. Microbiology. 159(Pt 6):1120-35. [PudMed:23519162]
[4] Bernard CS et al (2011) Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins. J Bacteriol. 193(9):2158-67. [PudMed:21378190]
[5] Khajanchi BK et al (2009) N-acylhomoserine lactones involved in quorum sensing control the type VI secretion system, biofilm formation, protease production, and in vivo virulence in a clinical isolate of Aeromonas hydrophila. Microbiology. 155(Pt 11):3518-31. [PudMed:19729404]
[6] Suarez G et al (2010) Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. Microbiology. 156(Pt 12):3678-88. [PudMed:20798163]
[7] Suarez G et al (2008) Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog. 44(4):344-61. [PudMed:18037263]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentFHAPAARTssATssBTssCTssDTssETssFTssGTssHTssITssJTssKTssLTssM
Number112111111221111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
AHA_RS09135 (rrtA) 1980299..1980856 - 558 WP_011705695.1 rhombosortase
AHA_RS09140 1980807..1981493 + 687 WP_011705696.1 DTW domain-containing protein
AHA_RS09145 (queC) 1981516..1982223 + 708 WP_011705697.1 7-cyano-7-deazaguanine synthase QueC
AHA_RS09150 (queE) 1982285..1982956 + 672 WP_045791049.1 7-carboxy-7-deazaguanine synthase QueE
AHA_RS09155 1982958..1983188 + 231 WP_011705699.1 DUF3820 family protein
AHA_RS09160 1983185..1983748 + 564 WP_011705700.1 cob(I)yrinic acid a,c-diamide adenosyltransferase
AHA_RS09165 1983815..1984219 - 405 WP_011705701.1 H-NS histone family protein
AHA_RS09170 1984799..1986379 + 1581 WP_011705702.1 Na+/H+ antiporter NhaC family protein
AHA_RS09175 1986466..1987047 + 582 WP_011705703.1 thymidine kinase
AHA_RS09180 1987116..1988453 - 1338 WP_011705704.1 flavohemoglobin expression-modulating QEGLA motif protein
AHA_RS09185 1988612..1989067 + 456 WP_164927603.1 YbjN domain-containing protein
T6CP008523 TssD AHA_RS09200 (hcp1) 1990515..1991033 + 519 WP_011705706.1 type VI secretion system effector Hcp1
T6CP001535 TssI AHA_RS09205 (vgrG) 1991311..1993542 + 2232 WP_011705707.1 type VI secretion system effector VgrG
AHA_RS09210 1993544..1994863 + 1320 WP_011705708.1 type VI secretion system polymorphic nuclease effector
AHA_RS09215 1994873..1995862 + 990 WP_011705709.1 type VI secretion system immunity protein
AHA_RS09220 1995977..1996522 + 546 WP_011705710.1 hypothetical protein
AHA_RS09225 1996658..1998031 + 1374 WP_164927604.1 sel1 repeat family protein
T6CP001536 TssB AHA_RS09230 (tssB) 1998704..1999207 + 504 WP_011705712.1 type VI secretion system contractile sheath small subunit
AHA_RS09235 (tssC) 1999246..2000724 + 1479 WP_043163044.1 type VI secretion system contractile sheath large subunit
T6CP008524 TssE AHA_RS09240 (tssE) 2000731..2001162 + 432 WP_011705714.1 type VI secretion system baseplate subunit TssE
T6CP001538 TssF AHA_RS09245 (tssF) 2001166..2002932 + 1767 WP_011705715.1 type VI secretion system baseplate subunit TssF
T6CP001539 TssG AHA_RS09250 (tssG) 2002896..2003894 + 999 WP_011705716.1 type VI secretion system baseplate subunit TssG
T6CP011548 FHA AHA_RS09255 (tagH) 2003951..2005201 + 1251 WP_011705717.1 type VI secretion system-associated FHA domain protein TagH
T6CP001540 TssJ AHA_RS09260 (tssJ) 2005201..2005716 + 516 WP_011705718.1 type VI secretion system lipoprotein TssJ
T6CP001541 TssK AHA_RS09265 (tssK) 2005719..2007053 + 1335 WP_011705719.1 type VI secretion system baseplate subunit TssK
AHA_RS09270 (icmH) 2007076..2007855 + 780 WP_010634228.1 type IVB secretion system protein IcmH/DotU
T6CP001543 TssH AHA_RS09275 (tssH) 2007876..2010518 + 2643 WP_011705721.1 type VI secretion system ATPase TssH
AHA_RS09280 (vasH) 2010521..2012059 + 1539 WP_011705722.1 sigma-54-dependent transcriptional regulator VasH
AHA_RS09285 (tagO) 2012059..2012664 + 606 WP_011705723.1 type VI secretion system-associated protein TagO
T6CP008525 TssA AHA_RS09290 (tssA) 2012673..2014109 + 1437 WP_011705724.1 type VI secretion system protein TssA
T6CP001544 TssM AHA_RS09295 (tssM) 2014151..2017636 + 3486 WP_011705725.1 type VI secretion system membrane subunit TssM
T6CP008526 TssA AHA_RS09300 2017683..2019113 + 1431 WP_011705726.1 type VI secretion system ImpA family N-terminal domain-containing protein
T6CP011549 PAAR AHA_RS09305 2019371..2019658 + 288 WP_011705727.1 type VI secretion system PAAR protein
T6CP001545 TssI AHA_RS09310 2019669..2021714 + 2046 WP_011705728.1 type VI secretion system tip protein VgrG
AHA_RS09315 2021749..2024304 + 2556 WP_011705729.1 hypothetical protein
AHA_RS09320 2024307..2024711 + 405 WP_011705730.1 DUF1311 domain-containing protein
AHA_RS09325 2025132..2025410 + 279 WP_139348863.1 hypothetical protein
AHA_RS09330 2025595..2025732 - 138 pseudogene IS5/IS1182 family transposase
AHA_RS09335 2025781..2026269 + 489 WP_139348864.1 hypothetical protein
AHA_RS09355 (folD) 2027356..2028219 + 864 WP_011705731.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
AHA_RS09360 (cspD) 2028327..2028545 - 219 WP_011705732.1 cold shock-like protein CspD
AHA_RS09365 (clpS) 2028774..2029091 + 318 WP_005300028.1 ATP-dependent Clp protease adapter ClpS
T6CP001546 TssH AHA_RS09370 (clpA) 2029151..2031403 + 2253 WP_011705733.1 ATP-dependent Clp protease ATP-binding subunit ClpA
AHA_RS09375 (infA) 2031473..2031691 - 219 WP_005300033.1 translation initiation factor IF-1
AHA_RS09380 2031761..2032477 - 717 WP_011705735.1 arginyltransferase
AHA_RS09385 (aat) 2032474..2033181 - 708 WP_011705736.1 leucyl/phenylalanyl-tRNA--protein transferase
AHA_RS09390 2033201..2033671 + 471 WP_011705737.1 glycine zipper 2TM domain-containing protein
AHA_RS09395 2033782..2035029 - 1248 WP_011705738.1 response regulator
AHA_RS09400 (trxB) 2035087..2036037 - 951 WP_164927605.1 thioredoxin-disulfide reductase
AHA_RS09405 2036554..2036784 - 231 WP_077392346.1 alanine dehydrogenase
AHA_RS09410 (lrp) 2036934..2037425 + 492 WP_011705740.1 leucine-responsive transcriptional regulator Lrp
AHA_RS09415 2037627..2040149 + 2523 WP_011705741.1 DNA translocase FtsK
AHA_RS09420 (lolA) 2040227..2040835 + 609 WP_011705742.1 outer membrane lipoprotein chaperone LolA
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0353 experimental AHA_1842 (VasH) protein QS regulatory pathway Aeromonas hydrophila subsp. hydrophila ATCC 7966 chromosome (NC_008570) 2010521..2012059 (+) 117617496 T6SS gene cluster promoter (PAHA1826) (↓)
REG0354 experimental AHA_3923 (rpoN) protein Global regulator Aeromonas hydrophila subsp. hydrophila ATCC 7966 chromosome (NC_008570) 4350676..4352115 (-) 117618923 T6SS gene cluster promoter (PAHA1826) (↓)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF00200 experimental AHA_RS05640 (VgrG1) Tre - Aeromonas hydrophila subsp. hydrophila ATCC 7966 chromosome (NC_008570) 1212752..1215535 (+) 117619461
EFF00201 experimental AHA_RS09205 (VgrG2) - - Aeromonas hydrophila subsp. hydrophila ATCC 7966 chromosome (NC_008570) 1991311..1993542 (+) 117621074
EFF00202 experimental AHA_RS09310 (VgrG3) - - Aeromonas hydrophila subsp. hydrophila ATCC 7966 chromosome (NC_008570) 2019669..2021714 (+) 117620092

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector