Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
T6SS ID | T6SS00157 |
T6SS type | Type i1 |
Strain | Aeromonas hydrophila subsp. hydrophila ATCC 7966 |
Replicon (RefSeq) | chromosome (NC_008570) |
Location | 1984799..2036268 |
Function | - |
Comment | This T6SS can secrete HCP. |
References | [1] Wang N et al (2015) Protective efficacy of recombinant hemolysin co-regulated protein (Hcp) of Aeromonas hydrophila in common carp (Cyprinus carpio). Fish Shellfish Immunol. 46(2):297-304. [PudMed:26093203] [2] Suarez G et al (2010) A type VI secretion system effector protein, VgrG1, from Aeromonas hydrophila that induces host cell toxicity by ADP ribosylation of actin. J Bacteriol. 192(1):155-68. [PudMed:19880608] [3] Sha J et al (2013) Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis. Microbiology. 159(Pt 6):1120-35. [PudMed:23519162] [4] Bernard CS et al (2011) Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins. J Bacteriol. 193(9):2158-67. [PudMed:21378190] [5] Khajanchi BK et al (2009) N-acylhomoserine lactones involved in quorum sensing control the type VI secretion system, biofilm formation, protease production, and in vivo virulence in a clinical isolate of Aeromonas hydrophila. Microbiology. 155(Pt 11):3518-31. [PudMed:19729404] [6] Suarez G et al (2010) Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. Microbiology. 156(Pt 12):3678-88. [PudMed:20798163] [7] Suarez G et al (2008) Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog. 44(4):344-61. [PudMed:18037263] |
T6SS components and genome coordinates
Component | FHA | PAAR | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM |
Number | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 1 | 1 |
Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
---|---|---|---|---|---|---|---|
AHA_RS09135 (rrtA) | 1980299..1980856 | - | 558 | WP_011705695.1 | rhombosortase | ||
AHA_RS09140 | 1980807..1981493 | + | 687 | WP_011705696.1 | DTW domain-containing protein | ||
AHA_RS09145 (queC) | 1981516..1982223 | + | 708 | WP_011705697.1 | 7-cyano-7-deazaguanine synthase QueC | ||
AHA_RS09150 (queE) | 1982285..1982956 | + | 672 | WP_045791049.1 | 7-carboxy-7-deazaguanine synthase QueE | ||
AHA_RS09155 | 1982958..1983188 | + | 231 | WP_011705699.1 | DUF3820 family protein | ||
AHA_RS09160 | 1983185..1983748 | + | 564 | WP_011705700.1 | cob(I)yrinic acid a,c-diamide adenosyltransferase | ||
AHA_RS09165 | 1983815..1984219 | - | 405 | WP_011705701.1 | H-NS histone family protein | ||
AHA_RS09170 | 1984799..1986379 | + | 1581 | WP_011705702.1 | Na+/H+ antiporter NhaC family protein | ||
AHA_RS09175 | 1986466..1987047 | + | 582 | WP_011705703.1 | thymidine kinase | ||
AHA_RS09180 | 1987116..1988453 | - | 1338 | WP_011705704.1 | flavohemoglobin expression-modulating QEGLA motif protein | ||
AHA_RS09185 | 1988612..1989067 | + | 456 | WP_164927603.1 | YbjN domain-containing protein | ||
T6CP008523 | TssD | AHA_RS09200 (hcp1) | 1990515..1991033 | + | 519 | WP_011705706.1 | type VI secretion system effector Hcp1 |
T6CP001535 | TssI | AHA_RS09205 (vgrG) | 1991311..1993542 | + | 2232 | WP_011705707.1 | type VI secretion system effector VgrG |
AHA_RS09210 | 1993544..1994863 | + | 1320 | WP_011705708.1 | type VI secretion system polymorphic nuclease effector | ||
AHA_RS09215 | 1994873..1995862 | + | 990 | WP_011705709.1 | type VI secretion system immunity protein | ||
AHA_RS09220 | 1995977..1996522 | + | 546 | WP_011705710.1 | hypothetical protein | ||
AHA_RS09225 | 1996658..1998031 | + | 1374 | WP_164927604.1 | sel1 repeat family protein | ||
T6CP001536 | TssB | AHA_RS09230 (tssB) | 1998704..1999207 | + | 504 | WP_011705712.1 | type VI secretion system contractile sheath small subunit |
AHA_RS09235 (tssC) | 1999246..2000724 | + | 1479 | WP_043163044.1 | type VI secretion system contractile sheath large subunit | ||
T6CP008524 | TssE | AHA_RS09240 (tssE) | 2000731..2001162 | + | 432 | WP_011705714.1 | type VI secretion system baseplate subunit TssE |
T6CP001538 | TssF | AHA_RS09245 (tssF) | 2001166..2002932 | + | 1767 | WP_011705715.1 | type VI secretion system baseplate subunit TssF |
T6CP001539 | TssG | AHA_RS09250 (tssG) | 2002896..2003894 | + | 999 | WP_011705716.1 | type VI secretion system baseplate subunit TssG |
T6CP011548 | FHA | AHA_RS09255 (tagH) | 2003951..2005201 | + | 1251 | WP_011705717.1 | type VI secretion system-associated FHA domain protein TagH |
T6CP001540 | TssJ | AHA_RS09260 (tssJ) | 2005201..2005716 | + | 516 | WP_011705718.1 | type VI secretion system lipoprotein TssJ |
T6CP001541 | TssK | AHA_RS09265 (tssK) | 2005719..2007053 | + | 1335 | WP_011705719.1 | type VI secretion system baseplate subunit TssK |
AHA_RS09270 (icmH) | 2007076..2007855 | + | 780 | WP_010634228.1 | type IVB secretion system protein IcmH/DotU | ||
T6CP001543 | TssH | AHA_RS09275 (tssH) | 2007876..2010518 | + | 2643 | WP_011705721.1 | type VI secretion system ATPase TssH |
AHA_RS09280 (vasH) | 2010521..2012059 | + | 1539 | WP_011705722.1 | sigma-54-dependent transcriptional regulator VasH | ||
AHA_RS09285 (tagO) | 2012059..2012664 | + | 606 | WP_011705723.1 | type VI secretion system-associated protein TagO | ||
T6CP008525 | TssA | AHA_RS09290 (tssA) | 2012673..2014109 | + | 1437 | WP_011705724.1 | type VI secretion system protein TssA |
T6CP001544 | TssM | AHA_RS09295 (tssM) | 2014151..2017636 | + | 3486 | WP_011705725.1 | type VI secretion system membrane subunit TssM |
T6CP008526 | TssA | AHA_RS09300 | 2017683..2019113 | + | 1431 | WP_011705726.1 | type VI secretion system ImpA family N-terminal domain-containing protein |
T6CP011549 | PAAR | AHA_RS09305 | 2019371..2019658 | + | 288 | WP_011705727.1 | type VI secretion system PAAR protein |
T6CP001545 | TssI | AHA_RS09310 | 2019669..2021714 | + | 2046 | WP_011705728.1 | type VI secretion system tip protein VgrG |
AHA_RS09315 | 2021749..2024304 | + | 2556 | WP_011705729.1 | hypothetical protein | ||
AHA_RS09320 | 2024307..2024711 | + | 405 | WP_011705730.1 | DUF1311 domain-containing protein | ||
AHA_RS09325 | 2025132..2025410 | + | 279 | WP_139348863.1 | hypothetical protein | ||
AHA_RS09330 | 2025595..2025732 | - | 138 | pseudogene | IS5/IS1182 family transposase | ||
AHA_RS09335 | 2025781..2026269 | + | 489 | WP_139348864.1 | hypothetical protein | ||
AHA_RS09355 (folD) | 2027356..2028219 | + | 864 | WP_011705731.1 | bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD | ||
AHA_RS09360 (cspD) | 2028327..2028545 | - | 219 | WP_011705732.1 | cold shock-like protein CspD | ||
AHA_RS09365 (clpS) | 2028774..2029091 | + | 318 | WP_005300028.1 | ATP-dependent Clp protease adapter ClpS | ||
T6CP001546 | TssH | AHA_RS09370 (clpA) | 2029151..2031403 | + | 2253 | WP_011705733.1 | ATP-dependent Clp protease ATP-binding subunit ClpA |
AHA_RS09375 (infA) | 2031473..2031691 | - | 219 | WP_005300033.1 | translation initiation factor IF-1 | ||
AHA_RS09380 | 2031761..2032477 | - | 717 | WP_011705735.1 | arginyltransferase | ||
AHA_RS09385 (aat) | 2032474..2033181 | - | 708 | WP_011705736.1 | leucyl/phenylalanyl-tRNA--protein transferase | ||
AHA_RS09390 | 2033201..2033671 | + | 471 | WP_011705737.1 | glycine zipper 2TM domain-containing protein | ||
AHA_RS09395 | 2033782..2035029 | - | 1248 | WP_011705738.1 | response regulator | ||
AHA_RS09400 (trxB) | 2035087..2036037 | - | 951 | WP_164927605.1 | thioredoxin-disulfide reductase | ||
AHA_RS09405 | 2036554..2036784 | - | 231 | WP_077392346.1 | alanine dehydrogenase | ||
AHA_RS09410 (lrp) | 2036934..2037425 | + | 492 | WP_011705740.1 | leucine-responsive transcriptional regulator Lrp | ||
AHA_RS09415 | 2037627..2040149 | + | 2523 | WP_011705741.1 | DNA translocase FtsK | ||
AHA_RS09420 (lolA) | 2040227..2040835 | + | 609 | WP_011705742.1 | outer membrane lipoprotein chaperone LolA |
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.
Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
---|---|---|---|---|---|---|---|---|
REG0353 | AHA_1842 (VasH) | protein | QS regulatory pathway | Aeromonas hydrophila subsp. hydrophila ATCC 7966 | chromosome (NC_008570) | 2010521..2012059 (+) | 117617496 | T6SS gene cluster promoter (PAHA1826) (↓) |
REG0354 | AHA_3923 (rpoN) | protein | Global regulator | Aeromonas hydrophila subsp. hydrophila ATCC 7966 | chromosome (NC_008570) | 4350676..4352115 (-) | 117618923 | T6SS gene cluster promoter (PAHA1826) (↓) |
Experimental investigation has been performed.
Bioinformatics investigation has been performed.
Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
---|---|---|---|---|---|---|---|
EFF00200 | AHA_RS05640 (VgrG1) | Tre | - | Aeromonas hydrophila subsp. hydrophila ATCC 7966 | chromosome (NC_008570) | 1212752..1215535 (+) | 117619461 |
EFF00201 | AHA_RS09205 (VgrG2) | - | - | Aeromonas hydrophila subsp. hydrophila ATCC 7966 | chromosome (NC_008570) | 1991311..1993542 (+) | 117621074 |
EFF00202 | AHA_RS09310 (VgrG3) | - | - | Aeromonas hydrophila subsp. hydrophila ATCC 7966 | chromosome (NC_008570) | 2019669..2021714 (+) | 117620092 |
Experimental investigation has been performed.
Bioinformatics investigation has been performed.
Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
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Experimental investigation has been performed.
Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
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