Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00260 experimental
T6SS type Type i3
Strain Yersinia pseudotuberculosis YPIII
Replicon (RefSeq) chromosome (NC_010465)
Location 3905709..3929297
Function effector translocation
Comment T6SS has an unexpected function in H+ extrusion under acid conditions.
References
[1] Wang T et al (2015) Type VI Secretion System Transports Zn2+ to Combat Multiple Stresses and Host Immunity. PLoS Pathog. 11(7):e1005020. [PudMed:26134274]
[2] Zhang W et al (2011) Modulation of a thermoregulated type VI secretion system by AHL-dependent quorum sensing in Yersinia pseudotuberculosis. Arch Microbiol. 193(5):351-63. [PudMed:21298257]
[3] Zhang W et al (2012) A type VI secretion system regulated by OmpR in Yersinia pseudotuberculosis functions to maintain intracellular pH homeostasis. Environ Microbiol. 15(2):557-69. [PudMed:23094603]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentPAARTssATssBTssCTssDTssETssFTssGTssHTssITssJTssKTssL
Number1111111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
YPK_RS17830 (rapA) 3902173..3905079 + 2907 WP_011191719.1 RNA polymerase-associated protein RapA
YPK_RS17835 3905334..3905687 - 354 WP_002210468.1 hypothetical protein
YPK_RS17840 (tssM) 3905709..3909150 - 3442 pseudogene type VI secretion system membrane subunit TssM
T6CP002565 TssL YPK_RS17845 3909212..3910822 - 1611 WP_012304591.1 type IVB secretion system protein IcmH/DotU
T6CP002566 TssK YPK_RS17850 (tssK) 3910819..3912174 - 1356 WP_002210470.1 type VI secretion system baseplate subunit TssK
T6CP002567 TssJ YPK_RS17855 (tssJ) 3912294..3912785 - 492 WP_012304592.1 type VI secretion system lipoprotein TssJ
T6CP011652 PAAR YPK_RS17860 3912778..3913143 - 366 WP_002214737.1 DUF4150 domain-containing protein
YPK_RS17865 3913149..3913766 - 618 WP_002210473.1 DUF3540 domain-containing protein
YPK_RS17870 3913759..3914862 - 1104 WP_002214739.1 pentapeptide repeat-containing protein
YPK_RS17875 3914888..3917107 - 2220 WP_002224087.1 pentapeptide repeat-containing protein
T6CP002568 TssI YPK_RS17880 (vgrG) 3917120..3919468 - 2349 WP_011191715.1 type VI secretion system tip protein VgrG
T6CP002569 TssH YPK_RS17885 (tssH) 3919572..3922160 - 2589 WP_002210475.1 type VI secretion system ATPase TssH
T6CP002570 TssG YPK_RS17890 (tssG) 3922178..3923161 - 984 WP_012304593.1 type VI secretion system baseplate subunit TssG
T6CP002571 TssF YPK_RS17895 (tssF) 3923154..3924998 - 1845 WP_012304594.1 type VI secretion system baseplate subunit TssF
T6CP002572 TssE YPK_RS17900 3925031..3925474 - 444 WP_002210478.1 type VI secretion system baseplate subunit TssE
T6CP002573 TssD YPK_RS17905 3925548..3926066 - 519 WP_012304595.1 type VI secretion system tube protein Hcp
T6CP002574 TssC YPK_RS17910 (tssC) 3926229..3927731 - 1503 WP_002210480.1 type VI secretion system contractile sheath large subunit
T6CP002575 TssB YPK_RS17915 (tssB) 3927740..3928300 - 561 WP_002210481.1 type VI secretion system contractile sheath small subunit
T6CP002576 TssA YPK_RS17920 3928311..3929324 - 1014 WP_002210482.1 ImpA family type VI secretion system protein
YPK_RS17925 3929654..3930397 + 744 WP_002214747.1 hexameric transcriptional regulator RovC
YPK_RS17930 (rluA) 3931620..3932240 + 621 WP_002210485.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA
YPK_RS17935 (djlA) 3932557..3933390 - 834 WP_012304596.1 co-chaperone DjlA
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0187 experimental YPK_3449 (RelA) protein (p)ppGpp regulatory pathway Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 3783755..3785989 (-) 488138165 YPK_1551 (RovM) (↓); YPK_1876 (RovA) (↓)
REG0188 experimental YPK_1876 (RovA) protein (p)ppGpp regulatory pathway Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 2082384..2082815 (+) 488139747 T6SS-4 promoter region (↓); T6SS-4 promoter region (↓)
REG0189 experimental YPK_4079 (OxyR) protein Global regulator Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 4502978..4503895 (-) 488138270 T6SS-4 promoter region (↓)
REG0190 experimental ypk_3425 (RpoS) protein Global regulator Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 3759239..3760237 (-) 499505140 T6SS-4 promoter region (↓)
REG0191 experimental YPK_1551 (RovM) protein (p)ppGpp regulatory pathway Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 1713682..1714884 (-) 499506038 T6SS-4 promoter region (↓)
REG0192 experimental YPK_3567 (RovC) protein carbon storage regulatory pathway Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 3929654..3930397 (+) 170025782 YPK_3567 promotor (↓)
REG0193 experimental YPK_3372 (CsrA) protein carbon storage regulatory pathway Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 3698608..3698793 (-) 169752121 YPK_3567 (RovC) (↑); YPK_3563 transcript (↑)
REG0194 experimental YPK_2462 (HpaR) protein Global regulator Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 2695107..2695574 (+) 169751221 T6SS4 promoter (↓)
REG0198 experimental YPK_RS03970 (ytbI) protein QS regulatory pathway Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 901200..901850 (-) 488144695 T6SS transcripts (↓); T6SS4 transcripts and hcp secretion (↓)
REG0199 experimental YPK_RS00840 (OmpR) protein TCS-RR Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 223260..223979 (+) 488137706 T6SS4 promoter (↓)
REG0200 experimental YPK_RS01520 (ZntR) protein Global regulator Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 333471..333896 (+) 488144494 T6SS4 promoter (↓)
REG0366 experimental YPK_RS19410 (Zur) protein Global regulator Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 4251751..4252260 (+) 488143439 T6SS4 transcription (↓)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF01462 experimental YPK_RS17835 Tie - Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 3905334..3905687 (-) 169752298
EFF00321 experimental YPK_RS17880 - - Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 3917120..3919468 (-) 170025773
EFF00322 experimental YPK_RS17905 - - Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 3925548..3926066 (-) 170025778
EFF76649 experimental YPK_3548 (TssS) Tie - Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 3905041..3905187 (-) -

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
IMU00376 experimental YPK_0955 (Tci1) EFF01874 experimental Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 1070252..1070536 (+) 499506273

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector