SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002575
Component protein type	TssB
T6SS ID (Type)	T6SS00260 (Type i3)
Strain	Yersinia pseudotuberculosis YPIII
Replicon	chromosome
Sequence	Protein sequence (187 a.a.); Nucleotide sequence (561 bp)
Reference	[1] Zhang W, Xu S, Li J, et al. Modulation of a thermoregulated type VI secretion system by AHL-dependent quorum sensing in Yersinia pseudotuberculosis. Arch Microbiol. 2011 May;193(5):351-63. doi: 10.1007/s00203-011-0680-2. Epub 2011 Feb 5. PMID: 21298257 [2] Zhang W, Wang Y, Song Y, et al. A type VI secretion system regulated by OmpR in Yersinia pseudotuberculosis functions to maintain intracellular pH homeostasis. Environ Microbiol. 2013 Feb;15(2):557-69. doi: 10.1111/1462-2920.12005. Epub 2012 Oct 24. PMID: 23094603 [3] Wang T, Si M, Song Y, et al. Type VI Secretion System Transports Zn2+ to Combat Multiple Stresses and Host Immunity. PLoS Pathog. 2015 Jul 2;11(7):e1005020. doi: 10.1371/journal.ppat.1005020. eCollection 2015 Jul. PMID: 26134274

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPK_RS17915
Coordinate (Strand)	3927740..3928300 (-)
NCBI ID	WP_002210481.1
RefSeq	NC_010465
Uniprot ID	Q66EQ1_YERPS, A0A0U1QWX4_YERP3, A0A0E1NXP0_YERPA, A0A0H2YDR0_YERPN, A0A0H3B806_YERPY, A0A2U2H085_YERPE, A0A3G5IR31_YERPU
KEGG ID	ypa:YPA_4095, ypq:DJ40_1728, ypn:YPN_0375, ypc:BZ23_117, yps:YPTB0641, ypo:BZ17_1916, ypu:BZ21_4023, ypr:BZ20_1454, ypy:YPK_3565, ypi:YpsIP31758_3436, ype:YPO0500
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	1.6e-54	13..163

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-186	MAKDASSQKFIGRNNAPRVQIEYDVEIYGSNKKVELPFVTGVMADLSGHRAKPLPAVEDRKFLTFDQDNFDARMRAIKPRLKFYVDNTLSEDNELLDIELDFESMDDFSPGSLARRIPQMEKLLEARTHLAELITYMDGKTSAEEVVKQLLEQPDFLQNLATDAQGTISKLSQDDDVLVEDDDGEK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9956	-	-

Protein sequence: 187 a.a.

						>T6CP002575 NC_010465:c3928300-3927740 [Yersinia pseudotuberculosis YPIII] [TssB]
MAKDASSQKFIGRNNAPRVQIEYDVEIYGSNKKVELPFVTGVMADLSGHRAKPLPAVEDRKFLTFDQDNFDARMRAIKPR
LKFYVDNTLSEDNELLDIELDFESMDDFSPGSLARRIPQMEKLLEARTHLAELITYMDGKTSAEEVVKQLLEQPDFLQNL
ATDAQGTISKLSQDDDVLVEDDDGEK*					

Nucleotide sequence: 561 bp

						>T6CP002575 NC_010465:c3928300-3927740 [Yersinia pseudotuberculosis YPIII] [TssB]
ATGGCCAAAGATGCGAGCTCGCAAAAATTTATCGGTAGAAATAATGCACCTAGAGTGCAAATTGAATATGACGTCGAAAT
TTATGGTTCAAATAAGAAAGTAGAGTTGCCTTTTGTAACCGGCGTGATGGCTGATCTTTCTGGCCACAGGGCAAAGCCTC
TTCCCGCCGTTGAAGATCGTAAATTCCTTACTTTCGATCAGGATAATTTCGATGCCCGGATGAGAGCAATTAAGCCTCGC
CTTAAATTCTACGTAGATAACACCTTGAGTGAGGATAACGAACTCCTGGATATCGAACTGGATTTTGAGTCAATGGATGA
CTTTTCTCCCGGATCGTTAGCGCGGCGTATTCCTCAAATGGAAAAACTGCTTGAGGCACGTACCCATCTGGCTGAATTGA
TCACCTATATGGATGGTAAAACCAGCGCAGAAGAGGTGGTTAAACAACTGCTTGAACAACCTGATTTTTTACAAAATCTT
GCGACCGATGCACAGGGAACTATTTCGAAGCTCAGCCAGGATGATGATGTTTTAGTGGAAGATGACGATGGAGAAAAATA
G					