Detailed information of regulator
Summary
| Regulator ID | REG0200  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Global regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00260 (Type i3) |
| Strain | Yersinia pseudotuberculosis YPIII |
| Replicon | chromosome |
| Sequence | Protein sequence (142 a.a.); Nucleotide sequence (426 bp) |
| Description | ZntR regulator specifically recognizes, binds to, and activates the T6SS4 promoter. |
| Reference |
External database links
| Locus tag (Gene) | YPK_RS01520 (ZntR) |
| Coordinates (Strand) | 333471..333896 (+) |
| NCBI ID | 488144494 |
| RefSeq | NC_010465 |
| Uniprot ID | Q664U8_YERPS, A0A0U1R028_YERP3, A0A0E1NXE1_YERPA, A0A0H2YMR5_YERPN, A0A0H3B0K2_YERPY |
| KEGG ID | ypo:BZ17_2916, ypn:YPN_3833, ypa:YPA_3237, ypy:YPK_0310, ypi:YpsIP31758_3888, yps:YPTB3671 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| T6SS4 promoter | ↓ | catcctgatttacatacctgttataggttatagctattctctgttgtgataggttatagctattatgtctttatttttttcttatttaagaaataacctaggacaaaaaacagtagtagggataaacttattcgcagattttttcacccttcatacatttattaaatttacataaccattagcacgatgacgtggatgaatagccaaaataagaggacatagatatgagaaagaagctatataatgacttcgcgtgggaatgcctgaggcgaaatccacaatatattagcgattgggaattatttatgaaaaatactcttactaatggagggggtatacccgatgattctgaattaatccaatcagagctggatttgaatgcagaaaaaaaatggggagtaatgaaatatattgatccttataactctgacccaac putative ZntR binding region is indicated red |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| MerR_1 | PF13411.8 | 7.4e-21 | 3..69 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-141 | MLKIGQLAKLADVTPDTIRYYEKQGMMDHGERTDGGYRLYTDQDLQRLRFIRYAKQLGFTLDTIAELLSIRIDPDHHTCQESKSIVDSRLSDVEGKIRELERMRDSLKRLSEACCGTSHATTYCSILEALEQGATEELIKV |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9924 | - | - |
Protein sequence of regulator: 142 a.a. .
>REG0200 NC_010465:333471-333896 [Yersinia pseudotuberculosis YPIII]
MLKIGQLAKLADVTPDTIRYYEKQGMMDHGERTDGGYRLYTDQDLQRLRFIRYAKQLGFTLDTIAELLSIRIDPDHHTCQ
ESKSIVDSRLSDVEGKIRELERMRDSLKRLSEACCGTSHATTYCSILEALEQGATEELIKV*
MLKIGQLAKLADVTPDTIRYYEKQGMMDHGERTDGGYRLYTDQDLQRLRFIRYAKQLGFTLDTIAELLSIRIDPDHHTCQ
ESKSIVDSRLSDVEGKIRELERMRDSLKRLSEACCGTSHATTYCSILEALEQGATEELIKV*
Nucleotide sequence of regulator: 426 bp .
>REG0200 NC_010465:333471-333896 [Yersinia pseudotuberculosis YPIII]
ATGTTAAAAATCGGGCAGTTAGCTAAACTTGCTGATGTAACGCCGGATACTATCCGTTACTACGAAAAGCAGGGAATGAT
GGATCACGGTGAACGAACTGATGGTGGCTACCGGCTGTATACTGATCAGGACCTTCAGCGTTTACGGTTTATCCGCTACG
CCAAGCAACTAGGCTTTACATTGGATACTATCGCAGAATTATTGTCGATCCGGATAGATCCTGACCATCATACCTGCCAG
GAGTCCAAGTCTATTGTAGATTCACGGCTTAGTGATGTGGAAGGTAAGATCAGAGAATTAGAGAGAATGCGTGATTCCCT
AAAAAGACTCAGCGAGGCTTGTTGTGGCACTAGTCATGCGACGACATACTGCTCAATATTGGAAGCTTTGGAGCAGGGGG
CAACAGAAGAGTTAATTAAGGTATGA
ATGTTAAAAATCGGGCAGTTAGCTAAACTTGCTGATGTAACGCCGGATACTATCCGTTACTACGAAAAGCAGGGAATGAT
GGATCACGGTGAACGAACTGATGGTGGCTACCGGCTGTATACTGATCAGGACCTTCAGCGTTTACGGTTTATCCGCTACG
CCAAGCAACTAGGCTTTACATTGGATACTATCGCAGAATTATTGTCGATCCGGATAGATCCTGACCATCATACCTGCCAG
GAGTCCAAGTCTATTGTAGATTCACGGCTTAGTGATGTGGAAGGTAAGATCAGAGAATTAGAGAGAATGCGTGATTCCCT
AAAAAGACTCAGCGAGGCTTGTTGTGGCACTAGTCATGCGACGACATACTGCTCAATATTGGAAGCTTTGGAGCAGGGGG
CAACAGAAGAGTTAATTAAGGTATGA