Detailed information of regulator


Summary

Regulator ID REG0192 experimental
Regulator type protein
Regulatory pathway (from literatures) carbon storage regulatory pathway
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00260 experimental (Type i3)
Strain Yersinia pseudotuberculosis YPIII
Replicon chromosome
Sequence Protein sequence (248 a.a.); Nucleotide sequence (744 bp)
Description CsrA represses expression of the T6SS4 operon through a novel regulator encoded by YPK_3567, which acts as an activator of the T6SS4 operon. RovC was able to specifically bind and form RovC-DNA complexes with DNA fragments harboring the intergenic region between the transcriptional start sites ofthe T6SS4 operon and the rovC gene and DNase I footprinting assays and found that RovC binds to a 39 bp sequence with a palindromic sequence immediately upstream ofthe -35 region ofthe T6SS4 promoter.
Reference
External database links

Locus tag (Gene) YPK_3567 (RovC)
Coordinates (Strand) 3929654..3930397 (+)
NCBI ID 170025782
RefSeq NC_010465
Uniprot ID UPI00000DC9A5
KEGG ID -
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
YPK_3567 promotor ↓ tagtagggataaacttattcgcagattttttcacccttc
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
DUF2285 PF10074.11 7.4e-11 183..247
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-247 MRKKLYNDFAWECLRRNPQYISDWELFMKNTLTNGGGIPDDSELIQSELDLNAEKKWGVMKYIDPYNSDPTNVFWSLKLSNRSVRVKLSNTGNVKGGYTWGDMSNLPGVKHQRLLMHDNTLCVKIFSQNGYFQLFIESADALKDDSNLYIYIPLNLESDVFAKNIELLQSIVNHKIEVECKEEQYLGLLKTIDDRKQGFSHRDIASEIFGKELVKNEWSADSWVRAKIRYRIKKANALINYGYLNFL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9642 - -


  Protein sequence of regulator: 248 a.a.    .

>REG0192 NC_010465:3929654-3930397 [Yersinia pseudotuberculosis YPIII]
MRKKLYNDFAWECLRRNPQYISDWELFMKNTLTNGGGIPDDSELIQSELDLNAEKKWGVMKYIDPYNSDPTNVFWSLKLS
NRSVRVKLSNTGNVKGGYTWGDMSNLPGVKHQRLLMHDNTLCVKIFSQNGYFQLFIESADALKDDSNLYIYIPLNLESDV
FAKNIELLQSIVNHKIEVECKEEQYLGLLKTIDDRKQGFSHRDIASEIFGKELVKNEWSADSWVRAKIRYRIKKANALIN
YGYLNFL*

  Nucleotide sequence of regulator: 744 bp    .

>REG0192 NC_010465:3929654-3930397 [Yersinia pseudotuberculosis YPIII]
ATGAGAAAGAAGCTATATAATGACTTCGCGTGGGAATGCCTGAGGCGAAATCCACAATATATTAGCGATTGGGAATTATT
TATGAAAAATACTCTTACTAATGGAGGGGGTATACCCGATGATTCTGAATTAATCCAATCAGAGCTGGATTTGAATGCAG
AAAAAAAATGGGGAGTAATGAAATATATTGATCCTTATAACTCTGACCCAACAAATGTTTTTTGGTCACTAAAATTAAGT
AACCGTTCAGTTAGAGTTAAACTCAGTAATACTGGAAATGTCAAAGGGGGATATACATGGGGAGATATGTCCAACCTACC
AGGAGTCAAACATCAACGCCTTCTTATGCATGACAATACACTGTGCGTAAAAATATTTAGTCAGAATGGGTATTTTCAGC
TTTTTATCGAGAGTGCAGATGCATTAAAAGATGATTCAAACCTCTATATTTATATCCCTTTGAACCTTGAATCTGATGTT
TTTGCTAAAAATATTGAGTTATTACAGAGCATTGTTAATCACAAAATAGAGGTGGAATGCAAAGAGGAGCAATATTTAGG
TCTCTTGAAGACCATAGATGACAGAAAACAAGGCTTCTCCCACCGGGATATCGCTTCAGAAATTTTTGGTAAGGAACTGG
TAAAAAATGAATGGTCTGCTGACAGTTGGGTCAGAGCAAAAATCCGCTATCGTATAAAGAAGGCGAATGCACTTATTAAT
TACGGCTACCTGAACTTCCTCTAG