Detailed information of regulator
Summary
| Regulator ID | REG0194  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Global regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00260 (Type i3) |
| Strain | Yersinia pseudotuberculosis YPIII |
| Replicon | chromosome |
| Sequence | Protein sequence (156 a.a.); Nucleotide sequence (468 bp) |
| Description | HpaR benefited Y. pseudotuberculosis resisting oxidative stress by upregulating T6SS4. DNase I footprinting analysis revealed that the binding site was protected from digestion in DNA–HpaR complexes and the interactions between HpaR and the T6SS4 promoter appeared to be site-specific, as mutation of the HpaR binding site in the T6SS4 promoter prevented formation of the protein–DNA complex. |
| Reference |
External database links
| Locus tag (Gene) | YPK_2462 (HpaR) |
| Coordinates (Strand) | 2695107..2695574 (+) |
| NCBI ID | 169751221 |
| RefSeq | NC_010465 |
| Uniprot ID | A0A0H3B3V2_YERPY |
| KEGG ID | ypy:YPK_2462 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| T6SS4 promoter | ↓ | CCTCTTATTTTGGCTATTCATCCACGTCATCGTGCTA |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| MarR | PF01047.24 | 2.5e-11 | 29..86 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-155 | MHESLTIALLQAREAAMAFFRPILKANHLTEQQWRIIRVLADNRSIDFHGLARQTCILRPSLTGILTRMERNNLIFRLKPVNDQRKLYVSLTQEGQNLYDNARPQVEEGYRLIEAAFSPDKMAQLSLLLDEFITVGNKHRQANEMSENEMLEDEE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9866 | - | - |
Protein sequence of regulator: 156 a.a. .
>REG0194 NC_010465:2695107-2695574 [Yersinia pseudotuberculosis YPIII]
MHESLTIALLQAREAAMAFFRPILKANHLTEQQWRIIRVLADNRSIDFHGLARQTCILRPSLTGILTRMERNNLIFRLKP
VNDQRKLYVSLTQEGQNLYDNARPQVEEGYRLIEAAFSPDKMAQLSLLLDEFITVGNKHRQANEMSENEMLEDEE*
MHESLTIALLQAREAAMAFFRPILKANHLTEQQWRIIRVLADNRSIDFHGLARQTCILRPSLTGILTRMERNNLIFRLKP
VNDQRKLYVSLTQEGQNLYDNARPQVEEGYRLIEAAFSPDKMAQLSLLLDEFITVGNKHRQANEMSENEMLEDEE*
Nucleotide sequence of regulator: 468 bp .
>REG0194 NC_010465:2695107-2695574 [Yersinia pseudotuberculosis YPIII]
ATGCATGAATCACTGACCATCGCATTATTGCAGGCTCGTGAAGCCGCTATGGCTTTTTTTCGGCCTATCTTGAAAGCCAA
TCATCTGACTGAGCAGCAATGGCGGATCATTCGAGTGCTCGCGGATAATCGCTCAATTGATTTTCATGGGCTGGCCCGCC
AAACCTGTATTTTACGTCCAAGCCTAACGGGGATTTTGACGCGAATGGAGCGTAACAATTTGATTTTTCGCCTCAAACCC
GTCAACGATCAGCGCAAACTTTATGTCTCCTTAACTCAGGAGGGACAAAATTTGTATGACAACGCTCGGCCACAGGTTGA
AGAAGGTTACAGGCTAATTGAGGCAGCATTTTCACCAGATAAAATGGCGCAACTCTCTTTACTGCTGGATGAATTCATTA
CCGTGGGTAATAAACATCGGCAAGCGAATGAGATGTCAGAAAATGAGATGTTAGAAGATGAAGAGTGA
ATGCATGAATCACTGACCATCGCATTATTGCAGGCTCGTGAAGCCGCTATGGCTTTTTTTCGGCCTATCTTGAAAGCCAA
TCATCTGACTGAGCAGCAATGGCGGATCATTCGAGTGCTCGCGGATAATCGCTCAATTGATTTTCATGGGCTGGCCCGCC
AAACCTGTATTTTACGTCCAAGCCTAACGGGGATTTTGACGCGAATGGAGCGTAACAATTTGATTTTTCGCCTCAAACCC
GTCAACGATCAGCGCAAACTTTATGTCTCCTTAACTCAGGAGGGACAAAATTTGTATGACAACGCTCGGCCACAGGTTGA
AGAAGGTTACAGGCTAATTGAGGCAGCATTTTCACCAGATAAAATGGCGCAACTCTCTTTACTGCTGGATGAATTCATTA
CCGTGGGTAATAAACATCGGCAAGCGAATGAGATGTCAGAAAATGAGATGTTAGAAGATGAAGAGTGA