Detailed information of regulator


Summary

Regulator ID REG0190 experimental
Regulator type protein
Regulatory pathway (from literatures) Global regulator
KEGG pathway (determined by BlastKOALA) Biofilm formation - Vibrio cholerae (map05111); Biofilm formation - Escherichia coli (map02026)
Related T6SS T6SS00260 experimental (Type i3)
Strain Yersinia pseudotuberculosis YPIII
Replicon chromosome
Sequence Protein sequence (333 a.a.); Nucleotide sequence (999 bp)
Description RpoS positively regulates T6SS4 expression by directly binding to the T6SS4 promoter.
Reference
External database links

Locus tag (Gene) ypk_3425 (RpoS)
Coordinates (Strand) 3759239..3760237 (-)
NCBI ID 499505140
RefSeq NC_010465
Uniprot ID A0A0E8XEN3_9GAMM, Q66EB7_YERPS, A0A0U1QXG5_YERP3, A0A0H3B6U5_YERPY, A0A0T9JH68_YERPU
KEGG ID ypo:BZ17_1780, ypc:BZ23_252, ypu:BZ21_4159, yps:YPTB0776, ypy:YPK_3425, ypr:BZ20_1309, ypi:YpsIP31758_3293
KEGG pathway (determined by BlastKOALA) map05111; map02026
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
T6SS-4 promoter region ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Sigma70_r2 PF04542.16 4.3e-21 96..165
Sigma70_r3 PF04539.18 1.2e-20 176..250
Sigma70_r4 PF04545.18 2.6e-15 264..317
Sigma70_r1_2 PF00140.22 5.7e-11 58..89
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-332 MSQSTLKVNELHEDADFDENSTETEIFDEKALVDDEPTESELADDELLAQGVTQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVPSRRRMIESNLRLVVKIARRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILSDENENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLSIEALFRE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9943 - -


  Protein sequence of regulator: 333 a.a.    .

>REG0190 NC_010465:c3760237-3759239 [Yersinia pseudotuberculosis YPIII]
MSQSTLKVNELHEDADFDENSTETEIFDEKALVDDEPTESELADDELLAQGVTQRVLDATQLYLGEIGYSPLLTAEEEVY
FARRALRGDVPSRRRMIESNLRLVVKIARRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAI
MNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDIL
SDENENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQ
TQGLSIEALFRE*

  Nucleotide sequence of regulator: 999 bp    .

>REG0190 NC_010465:c3760237-3759239 [Yersinia pseudotuberculosis YPIII]
ATGAGCCAAAGTACGCTGAAAGTTAACGAGTTGCATGAAGATGCTGATTTTGATGAGAACAGTACGGAAACTGAGATTTT
TGATGAAAAAGCATTAGTAGATGATGAACCTACTGAAAGCGAGTTAGCAGACGATGAGTTGTTGGCGCAAGGTGTTACCC
AGCGTGTGCTGGATGCGACGCAACTCTATCTTGGTGAAATCGGTTATTCGCCACTGCTAACGGCCGAAGAAGAGGTTTAT
TTTGCCCGGCGTGCATTGCGCGGAGACGTACCTTCACGTCGACGTATGATTGAGAGTAACTTGCGGCTGGTGGTGAAGAT
CGCCCGCCGTTACAGTAATCGCGGTTTAGCGCTGTTGGATCTGATAGAAGAGGGTAACCTCGGTTTGATCCGTGCGGTGG
AAAAGTTTGATCCTGAACGCGGTTTCCGTTTTTCCACTTACGCGACTTGGTGGATTCGCCAGACGATTGAACGGGCGATT
ATGAACCAAACCCGTACCATCCGTTTGCCTATCCATATCGTTAAAGAGTTAAACGTTTATTTACGTACAGCACGAGAGCT
TTCTCATAAATTGGATCATGAACCGAGCGCGGAAGAGATTGCAGAGCAACTCGACAAGCCGGTCGATGACGTGAGCCGTA
TGTTACGCCTTAACGAACGTATCACTTCTGTTGATACGCCTTTAGGCGGCGACTCAGAGAAAGCGTTGTTGGATATTCTG
TCTGACGAAAATGAGAACGGTCCGGAAGATACAACACAAGATGACGATATGAAACAGAGTATCGTTAAGTGGTTGTTTGA
GTTGAATGCAAAACAGCGCGAAGTGTTGGCCCGTCGTTTTGGTCTGTTAGGATATGAAGCGGCAACATTGGAAGATGTTG
GCCGAGAAATTGGTTTGACACGCGAGCGTGTCCGCCAGATTCAGGTTGAAGGCTTACGCCGTCTGCGGGAAATCCTGCAA
ACGCAAGGCCTGAGCATCGAGGCGTTGTTCCGCGAATAA