Detailed information of regulator
Summary
| Regulator ID | REG0188  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | (p)ppGpp regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00260 (Type i3) |
| Strain | Yersinia pseudotuberculosis YPIII |
| Replicon | chromosome |
| Sequence | Protein sequence (144 a.a.); Nucleotide sequence (432 bp) |
| Description | RovA positively regulates T6SS4 expression by recognizing two conserved AT-rich RovA-binding sites in the T6SS4 promoter. |
| Reference |
External database links
| Locus tag (Gene) | YPK_1876 (RovA) |
| Coordinates (Strand) | 2082384..2082815 (+) |
| NCBI ID | 488139747 |
| RefSeq | NC_010465 |
| Uniprot ID | SLYA_YERPS, SLYA_YERPG, SLYA_YERP3, SLYA_YERPN, SLYA_YERPP, SLYA_YERPY, SLYA_YERPE |
| KEGG ID | ypy:YPK_1876, yps:YPTB2288, ypp:YPDSF_0772, ypg:YpAngola_A2560, ypm:YP_2160, ypn:YPN_1831, ypi:YpsIP31758_1767, ypo:BZ17_172, ype:YPO2374, ypk:y1961 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | 4AIH, 4AIJ, 4AIK |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| T6SS-4 promoter region | ↓ | cctaggacaaaaaacagtagtagggataaacttattcgcagattttttcacccttcatacatttattaaatttacataaccattagcacgat |
| T6SS-4 promoter region | ↓ | tgatttataacctccctgtttgtatattttataatcatcctgatttacatacctgttat |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| MarR | PF01047.24 | 4e-17 | 29..87 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-143 | MESTLGSDLARLVRVWRALIDHRLKPLELTQTHWVTLYNINRLPPEQSQIQLAKAIGIEQPSLVRTLDQLEEKGLITRHTCANDRRAKRIKLTEQSSPIIEQVDGVICSTRKEILGGISSDEIAVLSGLIDKLEKNIIQLQTK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9985 | - | - |
Protein sequence of regulator: 144 a.a. .
>REG0188 NC_010465:2082384-2082815 [Yersinia pseudotuberculosis YPIII]
MESTLGSDLARLVRVWRALIDHRLKPLELTQTHWVTLYNINRLPPEQSQIQLAKAIGIEQPSLVRTLDQLEEKGLITRHT
CANDRRAKRIKLTEQSSPIIEQVDGVICSTRKEILGGISSDEIAVLSGLIDKLEKNIIQLQTK*
MESTLGSDLARLVRVWRALIDHRLKPLELTQTHWVTLYNINRLPPEQSQIQLAKAIGIEQPSLVRTLDQLEEKGLITRHT
CANDRRAKRIKLTEQSSPIIEQVDGVICSTRKEILGGISSDEIAVLSGLIDKLEKNIIQLQTK*
Nucleotide sequence of regulator: 432 bp .
>REG0188 NC_010465:2082384-2082815 [Yersinia pseudotuberculosis YPIII]
TTGGAATCGACATTAGGATCTGATCTAGCACGATTAGTTCGCGTTTGGCGCGCATTAATTGACCATCGGCTGAAACCGTT
GGAACTGACTCAAACACATTGGGTTACTTTGTATAACATTAATCGTTTACCACCAGAGCAATCACAGATTCAACTGGCGA
AAGCGATTGGTATTGAACAACCATCATTGGTTAGAACCTTGGATCAACTGGAGGAAAAAGGTTTAATCACACGGCATACT
TGTGCAAATGATCGTCGTGCGAAGAGAATTAAATTAACGGAACAGTCTTCACCGATAATCGAACAGGTTGATGGCGTGAT
TTGTTCTACCCGCAAAGAAATACTTGGGGGGATTTCATCGGATGAAATTGCAGTGTTATCAGGCCTAATCGATAAGCTTG
AGAAAAATATTATTCAATTACAAACTAAGTAA
TTGGAATCGACATTAGGATCTGATCTAGCACGATTAGTTCGCGTTTGGCGCGCATTAATTGACCATCGGCTGAAACCGTT
GGAACTGACTCAAACACATTGGGTTACTTTGTATAACATTAATCGTTTACCACCAGAGCAATCACAGATTCAACTGGCGA
AAGCGATTGGTATTGAACAACCATCATTGGTTAGAACCTTGGATCAACTGGAGGAAAAAGGTTTAATCACACGGCATACT
TGTGCAAATGATCGTCGTGCGAAGAGAATTAAATTAACGGAACAGTCTTCACCGATAATCGAACAGGTTGATGGCGTGAT
TTGTTCTACCCGCAAAGAAATACTTGGGGGGATTTCATCGGATGAAATTGCAGTGTTATCAGGCCTAATCGATAAGCTTG
AGAAAAATATTATTCAATTACAAACTAAGTAA