Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00376  |
| Cognate effector | EFF01874 |
| Related T6SS (Type) | T6SS00260 (Type i3) |
| Strain | Yersinia pseudotuberculosis YPIII |
| Replicon | chromosome |
| Sequence | Protein sequence (95 a.a.); Nucleotide sequence (285 bp) |
| Description | Expression of YPK_0954, a 67-amino acid (aa) protein in E. coli results in significant growth inhibition. This growth inhibition was relieved by co-expression of the immediately downstream gene YPK_0955, which encodes a protein containing the colicin_immundomaininthe ypk_0954-ypk_0955 bicistron (Fig. 1c). This result suggests that YPK_0955 is the cognate immunity protein for YPK_0954. We |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | YPK_0955 (Tci1) |
| Coordinates (Strand) | 1070252..1070536 (+) |
| NCBI ID | 499506273 |
| RefSeq | NC_010465 |
| Uniprot ID | A0A0E8XP54_9GAMM, Q666Y0_YERPS, A0A0T9RNQ6_9GAMM, A0A0H3B1F5_YERPY, A0A0T9MZ29_YERPU |
| KEGG ID | ypu:BZ21_2431, ypr:BZ20_3049, ypc:BZ23_2710, ypq:DJ40_3364, ysi:BF17_02060, ypo:BZ17_3504, yps:YPTB3114, ypy:YPK_0955 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Colicin_immun | PF09204.12 | 3e-37 | 1..87 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-94 | MSIVILEFAKSFINERVSAQVFANAYIEFWRIERDQRICLKDNEKLSECLSSIFCLADLYNPDSDREEYELDDKQLYEQVLQLINKLNQDALNK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9937 | - | - |
Protein sequence: 95 a.a. .
>IMU00376 NC_010465:1070252-1070536 [Yersinia pseudotuberculosis YPIII]
MSIVILEFAKSFINERVSAQVFANAYIEFWRIERDQRICLKDNEKLSECLSSIFCLADLYNPDSDREEYELDDKQLYEQV
LQLINKLNQDALNK*
MSIVILEFAKSFINERVSAQVFANAYIEFWRIERDQRICLKDNEKLSECLSSIFCLADLYNPDSDREEYELDDKQLYEQV
LQLINKLNQDALNK*
Nucleotide sequence: 285 bp .
>IMU00376 NC_010465:1070252-1070536 [Yersinia pseudotuberculosis YPIII]
ATGAGCATAGTTATTTTAGAATTCGCTAAATCATTTATTAATGAAAGAGTGTCAGCGCAGGTTTTTGCGAATGCTTACAT
TGAGTTTTGGAGGATAGAGCGTGACCAACGTATCTGTCTTAAAGACAATGAAAAATTAAGCGAGTGCTTATCAAGTATAT
TTTGTTTGGCTGATCTCTATAATCCTGACTCAGACAGAGAGGAATATGAACTTGACGATAAGCAACTATATGAACAAGTT
CTACAGCTCATCAATAAACTAAATCAGGACGCTCTAAATAAATAG
ATGAGCATAGTTATTTTAGAATTCGCTAAATCATTTATTAATGAAAGAGTGTCAGCGCAGGTTTTTGCGAATGCTTACAT
TGAGTTTTGGAGGATAGAGCGTGACCAACGTATCTGTCTTAAAGACAATGAAAAATTAAGCGAGTGCTTATCAAGTATAT
TTTGTTTGGCTGATCTCTATAATCCTGACTCAGACAGAGAGGAATATGAACTTGACGATAAGCAACTATATGAACAAGTT
CTACAGCTCATCAATAAACTAAATCAGGACGCTCTAAATAAATAG