SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002567
Component protein type	TssJ
T6SS ID (Type)	T6SS00260 (Type i3)
Strain	Yersinia pseudotuberculosis YPIII
Replicon	chromosome
Sequence	Protein sequence (164 a.a.); Nucleotide sequence (492 bp)
Reference	[1] Zhang W, Xu S, Li J, et al. Modulation of a thermoregulated type VI secretion system by AHL-dependent quorum sensing in Yersinia pseudotuberculosis. Arch Microbiol. 2011 May;193(5):351-63. doi: 10.1007/s00203-011-0680-2. Epub 2011 Feb 5. PMID: 21298257 [2] Zhang W, Wang Y, Song Y, et al. A type VI secretion system regulated by OmpR in Yersinia pseudotuberculosis functions to maintain intracellular pH homeostasis. Environ Microbiol. 2013 Feb;15(2):557-69. doi: 10.1111/1462-2920.12005. Epub 2012 Oct 24. PMID: 23094603 [3] Wang T, Si M, Song Y, et al. Type VI Secretion System Transports Zn2+ to Combat Multiple Stresses and Host Immunity. PLoS Pathog. 2015 Jul 2;11(7):e1005020. doi: 10.1371/journal.ppat.1005020. eCollection 2015 Jul. PMID: 26134274

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPK_RS17855
Coordinate (Strand)	3912294..3912785 (-)
NCBI ID	WP_012304592.1
RefSeq	NC_010465
Uniprot ID	A0A0H3B6D5_YERPY
KEGG ID	ypy:YPK_3553
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	2.6e-18	37..152

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-4	MRSL
Transmembrane helix	5-27	FRVLPIFILTGCVLLSGCSWFSS
Inside	28-163	SSEAALQKRRIDLQVVAAPLINPGPNGQAQPLKVCIVELNKEGWSPPGLYQGTLCSGISVGGEVVSVTEYILAPTEVRQYSRDVPFEQERWWAIAAEFQEMSNGKSLLMLKSDARADFNRVVLVDGKALSLKASTK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9985	pos: 21-22. LSG-CS.	MRSLFRVLPIFILTGCVLLSG

Protein sequence: 164 a.a.

						>T6CP002567 NC_010465:c3912785-3912294 [Yersinia pseudotuberculosis YPIII] [TssJ]
MRSLFRVLPIFILTGCVLLSGCSWFSSSSEAALQKRRIDLQVVAAPLINPGPNGQAQPLKVCIVELNKEGWSPPGLYQGT
LCSGISVGGEVVSVTEYILAPTEVRQYSRDVPFEQERWWAIAAEFQEMSNGKSLLMLKSDARADFNRVVLVDGKALSLKA
STK*					

Nucleotide sequence: 492 bp

						>T6CP002567 NC_010465:c3912785-3912294 [Yersinia pseudotuberculosis YPIII] [TssJ]
ATGCGTAGCCTCTTTCGTGTGTTGCCTATTTTTATCCTGACAGGTTGCGTCCTGCTCAGTGGCTGTAGCTGGTTTTCCTC
GTCCTCAGAAGCGGCGCTACAGAAACGGCGGATTGACTTGCAGGTTGTTGCGGCTCCTCTCATCAATCCTGGGCCCAACG
GTCAGGCACAGCCCTTGAAGGTATGCATTGTTGAGCTGAATAAAGAGGGATGGTCACCCCCCGGTTTGTATCAGGGGACG
CTCTGCAGTGGCATCAGTGTAGGAGGTGAAGTCGTCAGCGTGACGGAATACATCCTTGCGCCGACAGAGGTGCGTCAATA
TTCCCGCGATGTTCCCTTTGAACAAGAGCGCTGGTGGGCAATAGCGGCTGAGTTTCAGGAGATGAGTAACGGTAAAAGCC
TATTAATGCTGAAATCTGATGCTCGTGCGGACTTCAACCGTGTGGTGCTCGTTGATGGTAAGGCCCTGTCATTAAAAGCG
TCAACAAAGTAG					