Detailed information of regulator


Summary

Regulator ID REG0193 experimental
Regulator type protein
Regulatory pathway (from literatures) carbon storage regulatory pathway
KEGG pathway (determined by BlastKOALA) Two-component system (map02020); Biofilm formation - Vibrio cholerae (map05111); Biofilm formation - Pseudomonas aeruginosa (map02025); Biofilm formation - Escherichia coli (map02026)
Related T6SS T6SS00260 experimental (Type i3)
Strain Yersinia pseudotuberculosis YPIII
Replicon chromosome
Sequence Protein sequence (62 a.a.); Nucleotide sequence (186 bp)
Description CsrA represses expression of the T6SS4 operon through a novel regulator encoded by YPK_3567, which acts as an activator of the T6SS4 operon.Northern blot and primer extension analysis demonstrated that rovC expression is repressed in the presence ofthe CsrA protein.; The transcript of YPK_3563, encoding Hcp was 8.71-fold more abundant in the ΔcsrA mutant strain in comparison to wildtype.
Reference
External database links

Locus tag (Gene) YPK_3372 (CsrA)
Coordinates (Strand) 3698608..3698793 (-)
NCBI ID 169752121
RefSeq NC_010465
Uniprot ID CSRA_YERPY
KEGG ID ypy:YPK_3372
KEGG pathway (determined by BlastKOALA) map02020; map05111; map02025; map02026
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
YPK_3567 (RovC) ↑ gaugacguggaugaauagccaaaauaagaggacauagauaugagaaagaagcuauauaaugacuucgcgugggaaug
YPK_3563 transcript ↑ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
CsrA PF02599.18 1.8e-26 1..52
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-61 MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHREEIYQRIQAEKSQPTTY


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9701 - -


  Protein sequence of regulator: 62 a.a.    .

>REG0193 NC_010465:c3698793-3698608 [Yersinia pseudotuberculosis YPIII]
MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHREEIYQRIQAEKSQPTTY*

  Nucleotide sequence of regulator: 186 bp    .

>REG0193 NC_010465:c3698793-3698608 [Yersinia pseudotuberculosis YPIII]
ATGCTTATTCTGACTCGTCGAGTTGGTGAAACACTCATGATTGGCGATGAGGTTACGGTTACTGTATTAGGGGTTAAAGG
CAACCAGGTTCGAATTGGTGTAAATGCTCCGAAAGAGGTTTCTGTTCACCGTGAAGAAATCTACCAGCGCATCCAAGCAG
AAAAGTCTCAACCGACGACTTACTGA