SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002571
Component protein type	TssF
T6SS ID (Type)	T6SS00260 (Type i3)
Strain	Yersinia pseudotuberculosis YPIII
Replicon	chromosome
Sequence	Protein sequence (615 a.a.); Nucleotide sequence (1845 bp)
Reference	[1] Zhang W, Xu S, Li J, et al. Modulation of a thermoregulated type VI secretion system by AHL-dependent quorum sensing in Yersinia pseudotuberculosis. Arch Microbiol. 2011 May;193(5):351-63. doi: 10.1007/s00203-011-0680-2. Epub 2011 Feb 5. PMID: 21298257 [2] Zhang W, Wang Y, Song Y, et al. A type VI secretion system regulated by OmpR in Yersinia pseudotuberculosis functions to maintain intracellular pH homeostasis. Environ Microbiol. 2013 Feb;15(2):557-69. doi: 10.1111/1462-2920.12005. Epub 2012 Oct 24. PMID: 23094603 [3] Wang T, Si M, Song Y, et al. Type VI Secretion System Transports Zn2+ to Combat Multiple Stresses and Host Immunity. PLoS Pathog. 2015 Jul 2;11(7):e1005020. doi: 10.1371/journal.ppat.1005020. eCollection 2015 Jul. PMID: 26134274

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPK_RS17895
Coordinate (Strand)	3923154..3924998 (-)
NCBI ID	WP_012304594.1
RefSeq	NC_010465
Uniprot ID	A0A0H3B747_YERPY
KEGG ID	ypy:YPK_3561
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	1.3e-122	4..609

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-614	MPNESFLALYNEELRYLREDGQRFAHMHPQVAQHLGLHADGVLDPFVERLLEGTAFLSSRIQQRLNNEHPEFALQMLGRLAPLWYTPLPSIATIAMVPDLTSPQWHSRVVLPKGSRVTLSDSSLNNRLANFSTGRTLKIQPLVIENAECASTPPAYLPLSVASQLQAGQAHLRLRLTTQGVASLSELDFAPMNLTLAGGIIQSNQLITTLLNNTLHIVLWANTDGKPLVKVLTKEHLRQGGLDEQEALLPVAISEMPGNRLLREYFAAPSRFFDLELHGLNDFLQQGDGIHTFEVLFVLDQRPLHLIDRVTGNDFRLFATPIINLFKRRCSPVLINGEHTEYPVIVDPLNPRLYEVHHVIGVNGLLSEGGSVQFSSLHGHARFDDDDKVAGYSIRRRRDFTDKNHQKNSPLPHDSLFIALSPGTSGIDIDHVKSLSIEAMVCERHLIPSQLQHPEFKLEIALPVGQIEMLRHPAKPQGVPNIDQAWRALQLIANNPLRYALPDVTDCSTLLKDWLSIFCQHEEASQHKRITSLTHASVAHNFERHTAPGPIAWSRGVEATLDFNSSHHSDKGAFLFARILHHALSEYCELGQTLRLHMQVDGETFAKWGPVNYG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9961	-	-

Protein sequence: 615 a.a.

						>T6CP002571 NC_010465:c3924998-3923154 [Yersinia pseudotuberculosis YPIII] [TssF]
MPNESFLALYNEELRYLREDGQRFAHMHPQVAQHLGLHADGVLDPFVERLLEGTAFLSSRIQQRLNNEHPEFALQMLGRL
APLWYTPLPSIATIAMVPDLTSPQWHSRVVLPKGSRVTLSDSSLNNRLANFSTGRTLKIQPLVIENAECASTPPAYLPLS
VASQLQAGQAHLRLRLTTQGVASLSELDFAPMNLTLAGGIIQSNQLITTLLNNTLHIVLWANTDGKPLVKVLTKEHLRQG
GLDEQEALLPVAISEMPGNRLLREYFAAPSRFFDLELHGLNDFLQQGDGIHTFEVLFVLDQRPLHLIDRVTGNDFRLFAT
PIINLFKRRCSPVLINGEHTEYPVIVDPLNPRLYEVHHVIGVNGLLSEGGSVQFSSLHGHARFDDDDKVAGYSIRRRRDF
TDKNHQKNSPLPHDSLFIALSPGTSGIDIDHVKSLSIEAMVCERHLIPSQLQHPEFKLEIALPVGQIEMLRHPAKPQGVP
NIDQAWRALQLIANNPLRYALPDVTDCSTLLKDWLSIFCQHEEASQHKRITSLTHASVAHNFERHTAPGPIAWSRGVEAT
LDFNSSHHSDKGAFLFARILHHALSEYCELGQTLRLHMQVDGETFAKWGPVNYG*					

Nucleotide sequence: 1845 bp

						>T6CP002571 NC_010465:c3924998-3923154 [Yersinia pseudotuberculosis YPIII] [TssF]
ATGCCCAACGAAAGTTTTCTGGCTCTATACAACGAAGAGTTGCGTTATCTGCGTGAAGATGGGCAGCGCTTCGCTCATAT
GCATCCGCAAGTCGCCCAACACCTTGGGTTACATGCTGATGGTGTGCTCGATCCTTTCGTCGAACGGCTCCTTGAAGGCA
CCGCTTTCTTGAGTTCCCGTATCCAACAGCGTTTGAACAACGAGCATCCAGAGTTTGCTTTGCAAATGCTTGGGCGTTTG
GCCCCACTTTGGTATACACCCCTGCCGTCGATTGCCACCATTGCCATGGTTCCGGATCTTACGTCACCGCAATGGCACTC
GCGCGTCGTATTGCCTAAAGGTAGCCGCGTGACATTGAGTGACAGTTCGCTGAACAACCGGTTGGCGAATTTCTCTACTG
GCCGCACGCTGAAAATACAGCCATTGGTCATTGAAAATGCCGAGTGTGCCAGCACGCCACCGGCGTATTTACCGCTCTCT
GTGGCTTCACAATTACAAGCTGGTCAGGCCCATCTGCGCTTGCGTCTGACCACTCAAGGTGTCGCGTCTTTATCTGAGCT
GGATTTCGCCCCGATGAACCTGACGCTGGCGGGGGGAATAATTCAGTCAAATCAGTTAATCACGACACTGTTGAATAACA
CCTTACATATTGTTTTGTGGGCCAACACGGATGGGAAACCGCTGGTCAAAGTTCTTACCAAAGAACATCTTCGCCAGGGA
GGGCTGGATGAACAAGAGGCGCTTTTACCTGTTGCGATCAGCGAAATGCCTGGAAACCGTTTGCTACGAGAATATTTTGC
TGCTCCTTCGCGTTTTTTCGATCTGGAACTGCATGGCCTCAACGACTTCCTGCAACAAGGGGACGGCATTCATACCTTTG
AGGTGCTGTTTGTACTGGATCAGCGCCCCCTACATTTGATTGATAGGGTCACTGGCAATGACTTCCGACTCTTCGCCACA
CCTATTATTAACCTGTTCAAACGCCGTTGTAGCCCGGTGCTGATTAATGGTGAACACACCGAATATCCCGTCATTGTTGA
CCCCCTGAACCCCCGCTTGTACGAAGTCCATCATGTAATCGGGGTCAACGGTTTGTTGAGCGAAGGGGGGAGCGTGCAGT
TTTCTTCCCTTCATGGTCATGCCCGTTTTGATGATGACGATAAAGTGGCGGGTTACAGCATTCGCCGTCGCCGCGACTTC
ACGGATAAAAATCATCAGAAAAATTCACCTTTACCTCATGACAGCTTATTCATTGCCCTTTCACCGGGGACCAGCGGGAT
AGATATTGATCACGTCAAGTCGCTGTCGATTGAAGCCATGGTGTGCGAACGCCACTTAATTCCCAGCCAATTACAGCATC
CGGAATTCAAGCTGGAAATTGCCCTGCCAGTGGGGCAGATCGAAATGCTGCGTCACCCGGCAAAACCACAGGGTGTGCCG
AATATTGATCAAGCCTGGCGGGCGTTACAACTGATTGCCAACAATCCGCTGCGTTATGCGCTGCCGGATGTCACCGATTG
TTCGACGCTGCTAAAAGATTGGCTGTCGATATTTTGTCAGCATGAAGAGGCGAGCCAGCATAAGAGGATCACTAGCCTGA
CTCACGCCAGTGTGGCGCATAATTTTGAACGTCATACCGCTCCGGGGCCGATCGCCTGGAGCCGGGGGGTTGAAGCCACA
CTGGATTTCAACAGCAGCCACCACAGTGATAAAGGTGCATTCCTCTTTGCGCGGATCCTGCATCACGCGCTGTCAGAATA
TTGTGAATTAGGGCAGACCCTGAGGTTACACATGCAGGTTGATGGTGAAACCTTCGCCAAATGGGGGCCTGTCAACTATG
GCTAG					