Detailed information of regulator


Summary

Regulator ID REG0256 experimental
Regulator type protein
Regulatory pathway (from literatures) QS regulatory pathway
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00788 experimental (Type i1)
Strain Aeromonas dhakensis strain SSU
Replicon chromosome
Sequence Protein sequence (261 a.a.); Nucleotide sequence (783 bp)
Description AHL mediated the QS-controlled secretion of Hcp and VgrG2 effectors of T6SS in A. hydrophila SSU
Reference
External database links

Locus tag (Gene) HMPREF1171_RS20345
Coordinates (Strand) 4528880..4529662 (+)
NCBI ID WP_005308457.1
RefSeq NZ_JH815591
Uniprot ID K1J5Z5_9GAMM, A0A261E9R4_9GAMM
KEGG ID -
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
hcp and vgrG2 expression ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Autoind_bind PF03472.17 4.7e-32 20..165
GerE PF00196.21 1.6e-13 182..235
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-260 MKQDQLLEYLEHFTSVTDGDRLAELIGRFTLGMGYDYYRFALIIPMSMQRPKVVLFNQCPDSWVQAYTANHMLACDPIIQLARKQTLPIYWNRLDERARFLQEGSLDVMGLAAEFGLRNGISFPLHGAAGENGILSFITAERASSDLLLESSPVLSWMSNYIFEAAIRIVRVSLRDDDPQEALTDRETECLFWASEGKTSSEIACILGITERTVNYHLNQVTRKTGSMNRYQAIAKGVSSGILLPNLEQVVVTNFPKLMQ


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9833 - -


  Protein sequence of regulator: 261 a.a.    .

>REG0256 NZ_JH815591:4528880-4529662 [Aeromonas dhakensis]
MKQDQLLEYLEHFTSVTDGDRLAELIGRFTLGMGYDYYRFALIIPMSMQRPKVVLFNQCPDSWVQAYTANHMLACDPIIQ
LARKQTLPIYWNRLDERARFLQEGSLDVMGLAAEFGLRNGISFPLHGAAGENGILSFITAERASSDLLLESSPVLSWMSN
YIFEAAIRIVRVSLRDDDPQEALTDRETECLFWASEGKTSSEIACILGITERTVNYHLNQVTRKTGSMNRYQAIAKGVSS
GILLPNLEQVVVTNFPKLMQ*

  Nucleotide sequence of regulator: 783 bp    .

>REG0256 NZ_JH815591:4528880-4529662 [Aeromonas dhakensis]
ATGAAACAAGACCAACTGCTTGAGTACCTCGAACATTTCACTTCGGTAACGGACGGGGATCGTCTGGCCGAGTTGATCGG
TCGGTTCACGCTCGGTATGGGTTACGACTACTACCGGTTTGCGCTGATCATTCCCATGTCGATGCAAAGACCCAAGGTGG
TGCTGTTCAATCAGTGCCCTGACTCCTGGGTGCAGGCCTATACGGCAAACCATATGCTGGCCTGTGATCCCATCATTCAA
TTGGCCCGCAAGCAGACCTTGCCCATTTACTGGAACCGGCTGGATGAGCGAGCACGTTTTCTGCAAGAGGGCAGTCTGGA
CGTGATGGGGTTGGCGGCGGAGTTCGGGCTGCGCAACGGCATCTCGTTTCCGTTGCACGGGGCGGCGGGAGAAAACGGCA
TACTGTCGTTCATCACCGCCGAGCGGGCATCGAGCGATCTGCTGCTGGAGTCCTCGCCCGTCCTCTCCTGGATGTCCAAC
TACATCTTCGAGGCGGCGATCCGGATTGTGCGGGTCAGTCTGCGGGACGATGACCCTCAGGAGGCCCTGACTGACCGGGA
GACCGAATGCCTGTTCTGGGCCAGTGAAGGGAAAACGTCGAGTGAAATCGCCTGCATCCTGGGGATCACGGAGCGAACCG
TGAACTACCACCTCAATCAGGTCACCCGTAAAACGGGGTCGATGAATCGCTACCAGGCCATCGCCAAGGGGGTGAGCAGT
GGCATCTTGCTGCCCAATCTCGAGCAGGTCGTGGTCACCAACTTCCCCAAGCTGATGCAATAA