Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00788  |
| T6SS type | Type i1 |
| Strain | Aeromonas dhakensis strain SSU |
| Replicon (RefSeq) | chromosome (NZ_JH815591) |
| Location | 1017876..1061054 |
| Function | anti-eukaryotic; effector translocation; virulence |
| Comment | Cytotoxicity and inhibition of phagocytosis in cultured macrophages and epithelial cells; full virulence in septicaemic mouse model. |
| References | [1] Suarez G et al (2008) Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog. 44(4):344-61. [PudMed:18037263] [2] Suarez G et al (2010) Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. Microbiology. 156(Pt 12):3678-88. [PudMed:20798163] [3] Grim CJ et al (2014) Functional Genomic Characterization of Virulence Factors from Necrotizing Fasciitis-Causing Strains of Aeromonas hydrophila. Appl Environ Microbiol. 80(14):4162-83. [PudMed:24795370] [4] Suarez G et al (2010) A type VI secretion system effector protein, VgrG1, from Aeromonas hydrophila that induces host cell toxicity by ADP ribosylation of actin. J Bacteriol. 192(1):155-68. [PudMed:19880608] [5] Sha J et al (2013) Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis. Microbiology. 159(Pt 6):1120-35. [PudMed:23519162] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | FHA | PAAR | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM |
| Number | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| HMPREF1171_RS04660 | 1014366..1014947 | + | 582 | WP_005299956.1 | thymidine kinase | ||
| HMPREF1171_RS04665 | 1015016..1016353 | - | 1338 | WP_005299958.1 | flavohemoglobin expression-modulating QEGLA motif protein | ||
| HMPREF1171_RS04670 | 1016512..1016967 | + | 456 | WP_005299960.1 | YbjN domain-containing protein | ||
| T6CP010911 | TssD | HMPREF1171_RS04680 (hcp1) | 1017876..1018309 | + | 434 | WP_005299962.1 | type VI secretion system effector Hcp1 |
| HMPREF1171_RS04685 (hcp1) | 1018410..1018589 | + | 180 | pseudogene | type VI secretion system effector Hcp1 | ||
| T6CP010912 | TssI | HMPREF1171_RS04690 | 1019048..1021076 | + | 2029 | WP_005299968.1 | type VI secretion system tip protein VgrG |
| HMPREF1171_RS04695 | 1021066..1021794 | + | 729 | WP_005299970.1 | DUF4123 domain-containing protein | ||
| HMPREF1171_RS04700 | 1021791..1026428 | + | 4638 | WP_005299972.1 | DUF6531 domain-containing protein | ||
| HMPREF1171_RS23520 | 1026425..1026889 | + | 465 | WP_154956505.1 | hypothetical protein | ||
| HMPREF1171_RS23525 | 1026961..1027392 | + | 432 | WP_154956506.1 | hypothetical protein | ||
| HMPREF1171_RS04705 | 1027446..1028828 | + | 1383 | WP_005299974.1 | SEL1-like repeat protein | ||
| T6CP259517 | TssB | HMPREF1171_RS04710 (tssB) | 1029519..1030022 | + | 504 | WP_005299975.1 | type VI secretion system contractile sheath small subunit |
| T6CP010913 | TssC | HMPREF1171_RS04715 (tssC) | 1030061..1031539 | + | 1479 | WP_005299978.1 | type VI secretion system contractile sheath large subunit |
| T6CP010914 | TssE | HMPREF1171_RS04720 (tssE) | 1031546..1031977 | + | 432 | WP_005299981.1 | type VI secretion system baseplate subunit TssE |
| T6CP010915 | TssF | HMPREF1171_RS04725 (tssF) | 1031981..1033747 | + | 1767 | WP_005299983.1 | type VI secretion system baseplate subunit TssF |
| T6CP010916 | TssG | HMPREF1171_RS04730 (tssG) | 1033711..1034709 | + | 999 | WP_005299985.1 | type VI secretion system baseplate subunit TssG |
| T6CP011346 | FHA | HMPREF1171_RS04735 (tagH) | 1034766..1036046 | + | 1281 | WP_005299988.1 | type VI secretion system-associated FHA domain protein TagH |
| T6CP010917 | TssJ | HMPREF1171_RS04740 (tssJ) | 1036046..1036561 | + | 516 | WP_005299990.1 | type VI secretion system lipoprotein TssJ |
| T6CP010918 | TssK | HMPREF1171_RS04745 (tssK) | 1036564..1037898 | + | 1335 | WP_005299993.1 | type VI secretion system baseplate subunit TssK |
| T6CP010919 | TssL | HMPREF1171_RS04750 (icmH) | 1037921..1038700 | + | 780 | WP_005299995.1 | type IVB secretion system protein IcmH/DotU |
| T6CP010920 | TssH | HMPREF1171_RS04755 (tssH) | 1038721..1041363 | + | 2643 | WP_005299997.1 | type VI secretion system ATPase TssH |
| HMPREF1171_RS04760 (vasH) | 1041366..1042904 | + | 1539 | WP_005299999.1 | sigma-54-dependent transcriptional regulator VasH | ||
| HMPREF1171_RS04765 (tagO) | 1042904..1043509 | + | 606 | WP_005300001.1 | type VI secretion system-associated protein TagO | ||
| T6CP010921 | TssA | HMPREF1171_RS04770 (tssA) | 1043518..1044963 | + | 1446 | WP_005300003.1 | type VI secretion system protein TssA |
| T6CP010922 | TssM | HMPREF1171_RS04775 (tssM) | 1045005..1048490 | + | 3486 | WP_005300004.1 | type VI secretion system membrane subunit TssM |
| T6CP010923 | TssA | HMPREF1171_RS04780 | 1048537..1049967 | + | 1431 | WP_005300006.1 | type VI secretion system ImpA family N-terminal domain-containing protein |
| T6CP011347 | PAAR | HMPREF1171_RS04785 | 1050225..1050512 | + | 288 | WP_005300007.1 | type VI secretion system PAAR protein |
| T6CP010924 | TssI | HMPREF1171_RS04790 | 1050523..1052571 | + | 2049 | WP_005300009.1 | type VI secretion system tip protein VgrG |
| HMPREF1171_RS04800 | 1054604..1055227 | + | 624 | WP_029305891.1 | lytic enzyme | ||
| HMPREF1171_RS23530 | 1055395..1055883 | + | 489 | WP_154956507.1 | hypothetical protein | ||
| HMPREF1171_RS23535 | 1055948..1056298 | - | 351 | WP_029305885.1 | hypothetical protein | ||
| HMPREF1171_RS04825 (folD) | 1057010..1057873 | + | 864 | WP_005300012.1 | bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD | ||
| HMPREF1171_RS04830 (cspD) | 1057980..1058198 | - | 219 | WP_005300025.1 | cold shock-like protein CspD | ||
| HMPREF1171_RS04835 (clpS) | 1058428..1058745 | + | 318 | WP_005300028.1 | ATP-dependent Clp protease adapter ClpS | ||
| T6CP259518 | TssH | HMPREF1171_RS04840 (clpA) | 1058805..1061054 | + | 2250 | WP_005300030.1 | ATP-dependent Clp protease ATP-binding subunit ClpA |
| HMPREF1171_RS04845 (infA) | 1061124..1061342 | - | 219 | WP_005300033.1 | translation initiation factor IF-1 | ||
| HMPREF1171_RS04850 | 1061411..1062127 | - | 717 | WP_005300035.1 | arginyltransferase | ||
| HMPREF1171_RS04855 (aat) | 1062124..1062831 | - | 708 | WP_005300037.1 | leucyl/phenylalanyl-tRNA--protein transferase | ||
| HMPREF1171_RS04860 | 1062851..1063321 | + | 471 | WP_005300039.1 | glycine zipper 2TM domain-containing protein | ||
| HMPREF1171_RS04865 | 1063426..1064673 | - | 1248 | WP_005300041.1 | diguanylate cyclase | ||
| HMPREF1171_RS04870 (trxB) | 1064731..1065681 | - | 951 | WP_005300043.1 | thioredoxin-disulfide reductase |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|---|---|---|---|---|---|---|---|
| REG0254 |
HMPREF1171_RS04760 | protein | Transcription regulator | Aeromonas dhakensis strain SSU | chromosome (NZ_JH815591) | 1041366..1042904 (+) | WP_005299999.1 | hcp expression (↓) |
| REG0255 |
HMPREF1171_RS20340 | protein | QS regulatory pathway | Aeromonas dhakensis strain SSU | chromosome (NZ_JH815591) | 4528194..4528817 (-) | WP_005308454.1 | ahyR (↓) |
| REG0256 |
HMPREF1171_RS20345 | protein | QS regulatory pathway | Aeromonas dhakensis strain SSU | chromosome (NZ_JH815591) | 4528880..4529662 (+) | WP_005308457.1 | hcp and vgrG2 expression (↓) |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF01888 |
HMPREF1171_RS04800 (TseP) | Tpe | IMU17302 |
Aeromonas dhakensis strain SSU | chromosome (NZ_JH815591) | 1054604..1055227 (+) | WP_029305891.1 |
| EFF18622 |
HMPREF1171_RS04700 (TseI) | Tde | IMU17301 |
Aeromonas dhakensis strain SSU | chromosome (NZ_JH815591) | 1021791..1026428 (+) | WP_005299972.1 |
| EFF76650 |
HMPREF1171_RS12055 (tseC) | Tse | IMU00361 |
Aeromonas dhakensis strain SSU | chromosome (NZ_JH815591) | 2753469..2756654 (-) | WP_005303625.1 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|
| IMU00361 |
HMPREF1171_RS12050 (tsiC) | EFF76650 |
Aeromonas dhakensis strain SSU | chromosome (NZ_JH815591) | 2752679..2753485 (-) | WP_005303621.1 |
| IMU17301 |
HMPREF1171_RS04705 (TsiI) | EFF18622 |
Aeromonas dhakensis strain SSU | chromosome (NZ_JH815591) | 1027446..1028828 (+) | WP_005299974.1 |
| IMU17302 |
HMPREF1171_RS23530 | EFF01888 |
Aeromonas dhakensis strain SSU | chromosome (NZ_JH815591) | 1055395..1055883 (+) | WP_154956507.1 |
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|---|---|---|---|---|---|---|---|
| ACP26819 |
HMPREF1171_RS04695 (TecI) | chaperone | Aeromonas dhakensis strain SSU | chromosome (NZ_JH815591) | 1021066..1021794 (+) | WP_005299970.1 | HMPREF1171_RS04690 | HMPREF1171_RS04700 (TseI) |