Detailed information of accessory protein
Summary
| Accessory protein ID | ACP26819  |
| Type | chaperone |
| Related T6SS (Type) | T6SS00788 (Type i1) |
| Strain | Aeromonas dhakensis strain SSU |
| Replicon | chromosome |
| Related structure protein | HMPREF1171_RS04690 |
| Related effector | HMPREF1171_RS04700 (TseI) |
| Description | TseI secretion requires VgrG1 and chaperone TecI through direct interaction |
| Reference | [1] Pei TT, Li H, Liang X, et al. Intramolecular chaperone-mediated secretion of an Rhs effector toxin by a type VI secretion system. Nat Commun. 2020 Apr 20;11(1):1865. doi: 10.1038/s41467-020-15774-z. PMID: 32313027 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | HMPREF1171_RS04695 (TecI) |
| Coordinates (Strand) | 1021066..1021794 (+) |
| NCBI ID | WP_005299970.1 |
| RefSeq | NZ_JH815591 |
| Uniprot ID | K1JFL8_9GAMM |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DUF4123 | PF13503.8 | 3.1e-20 | 19..132 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-242 | VKFEITPLEEWIQRQSAPLYAVLAGASDAAPLQNYYQLDGRETPRGLYLGTPYEDWHPVMPYLVALDKESPFIEWAQSTEASDWGWIVASEQPVDTLYAHLQGLTQIWHQGEAVFFRYWDGVYLKRILVQLGEGFTALLPGLSGIWVGGGSFEWAASEATAPRAFPWWELPPVIAATLARQDTGPLVNNLMQQLADQNGQLYWSFPEANLRCKVARFVSRHSSPDIDLFPALEAALINEVQA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.7808 | - | - |
Protein sequence: 243 a.a.
>ACP26819 NZ_JH815591:1021066-1021794 [Aeromonas dhakensis]
VKFEITPLEEWIQRQSAPLYAVLAGASDAAPLQNYYQLDGRETPRGLYLGTPYEDWHPVMPYLVALDKESPFIEWAQSTE
ASDWGWIVASEQPVDTLYAHLQGLTQIWHQGEAVFFRYWDGVYLKRILVQLGEGFTALLPGLSGIWVGGGSFEWAASEAT
APRAFPWWELPPVIAATLARQDTGPLVNNLMQQLADQNGQLYWSFPEANLRCKVARFVSRHSSPDIDLFPALEAALINEV
QA*
VKFEITPLEEWIQRQSAPLYAVLAGASDAAPLQNYYQLDGRETPRGLYLGTPYEDWHPVMPYLVALDKESPFIEWAQSTE
ASDWGWIVASEQPVDTLYAHLQGLTQIWHQGEAVFFRYWDGVYLKRILVQLGEGFTALLPGLSGIWVGGGSFEWAASEAT
APRAFPWWELPPVIAATLARQDTGPLVNNLMQQLADQNGQLYWSFPEANLRCKVARFVSRHSSPDIDLFPALEAALINEV
QA*
Nucleotide sequence: 729 bp
>ACP26819 NZ_JH815591:1021066-1021794 [Aeromonas dhakensis]
GTGAAATTTGAGATCACGCCGCTGGAAGAGTGGATACAGAGGCAATCAGCCCCTCTTTATGCTGTGCTGGCCGGAGCCAG
CGATGCGGCTCCCTTGCAGAATTACTACCAGCTCGATGGCCGCGAAACGCCACGCGGGCTCTATCTCGGCACCCCTTATG
AAGACTGGCATCCCGTCATGCCCTATCTGGTGGCCCTCGACAAGGAGAGCCCCTTTATCGAGTGGGCGCAGAGCACCGAG
GCAAGCGACTGGGGCTGGATAGTGGCAAGCGAGCAGCCTGTCGATACCCTCTATGCCCATCTGCAGGGGCTGACCCAGAT
CTGGCACCAGGGGGAAGCGGTGTTCTTCCGTTACTGGGATGGCGTTTATCTGAAGCGCATTCTGGTCCAGCTGGGAGAGG
GCTTCACCGCCCTACTGCCTGGGTTGAGCGGGATCTGGGTCGGGGGGGGCAGCTTCGAATGGGCGGCGTCAGAGGCTACA
GCACCACGCGCATTCCCCTGGTGGGAGTTACCCCCCGTCATTGCTGCGACCTTGGCGAGGCAGGATACCGGCCCCCTCGT
CAACAACCTGATGCAGCAGTTGGCGGATCAAAACGGTCAGCTCTACTGGTCATTTCCAGAGGCGAACCTGCGCTGCAAGG
TTGCTCGCTTTGTCTCCCGTCATTCATCCCCTGATATCGATCTGTTTCCAGCGCTGGAAGCGGCGCTGATCAATGAGGTT
CAAGCATGA
GTGAAATTTGAGATCACGCCGCTGGAAGAGTGGATACAGAGGCAATCAGCCCCTCTTTATGCTGTGCTGGCCGGAGCCAG
CGATGCGGCTCCCTTGCAGAATTACTACCAGCTCGATGGCCGCGAAACGCCACGCGGGCTCTATCTCGGCACCCCTTATG
AAGACTGGCATCCCGTCATGCCCTATCTGGTGGCCCTCGACAAGGAGAGCCCCTTTATCGAGTGGGCGCAGAGCACCGAG
GCAAGCGACTGGGGCTGGATAGTGGCAAGCGAGCAGCCTGTCGATACCCTCTATGCCCATCTGCAGGGGCTGACCCAGAT
CTGGCACCAGGGGGAAGCGGTGTTCTTCCGTTACTGGGATGGCGTTTATCTGAAGCGCATTCTGGTCCAGCTGGGAGAGG
GCTTCACCGCCCTACTGCCTGGGTTGAGCGGGATCTGGGTCGGGGGGGGCAGCTTCGAATGGGCGGCGTCAGAGGCTACA
GCACCACGCGCATTCCCCTGGTGGGAGTTACCCCCCGTCATTGCTGCGACCTTGGCGAGGCAGGATACCGGCCCCCTCGT
CAACAACCTGATGCAGCAGTTGGCGGATCAAAACGGTCAGCTCTACTGGTCATTTCCAGAGGCGAACCTGCGCTGCAAGG
TTGCTCGCTTTGTCTCCCGTCATTCATCCCCTGATATCGATCTGTTTCCAGCGCTGGAAGCGGCGCTGATCAATGAGGTT
CAAGCATGA