Detailed information of component protein


Summary

Component ID T6CP010922
Component protein type TssM
T6SS ID (Type) T6SS00788 experimental (Type i1)
Strain Aeromonas dhakensis strain SSU
Replicon chromosome
Sequence Protein sequence (1162 a.a.); Nucleotide sequence (3486 bp)
Reference
[1] Suarez G, Sierra JC, Sha J, et al. Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog. 2008 Apr;44(4):344-61. Epub 2007 Oct 24. PMID: 18037263
[2] Suarez G, Sierra JC, Erova TE, et al. A type VI secretion system effector protein, VgrG1, from Aeromonas hydrophila that induces host cell toxicity by ADP ribosylation of actin. J Bacteriol. 2010 Jan;192(1):155-68. doi: 10.1128/JB.01260-09. PMID: 19880608
[3] Sha J, Rosenzweig JA, Kozlova EV, et al. Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis. Microbiology (Reading). 2013 Jun;159(Pt 6):1120-1135. doi: 10.1099/mic.0.063495-0. Epub 2013 Mar 21. PMID: 23519162
[4] Suarez G, Sierra JC, Kirtley ML, et al. Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. Microbiology (Reading). 2010 Dec;156(Pt 12):3678-3688. doi: 10.1099/mic.0.041277-0. Epub 2010 Aug 26. PMID: 20798163
[5] Grim CJ, Kozlova EV, Ponnusamy D, et al. Functional genomic characterization of virulence factors from necrotizing fasciitis-causing strains of Aeromonas hydrophila. Appl Environ Microbiol. 2014 Jul;80(14):4162-83. doi: 10.1128/AEM.00486-14. Epub 2014 May 2. PMID: 24795370
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) HMPREF1171_RS04775
Coordinate (Strand) 1045005..1048490 (+)
NCBI ID WP_005300004.1
RefSeq NZ_JH815591
Uniprot ID K1JMC6_9GAMM
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 2e-74 502..815
IcmF-related_N PF14331.8 6.2e-72 201..450
IcmF_C PF06744.14 3.8e-22 1034..1141
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-19 MFKTIFTFLRQQLPKLKPS
Transmembrane helix 20-39 WPLLGAVLWVLALILVWWLG
Outside 40-53 PRLELRGAKPFEPL
Transmembrane helix 54-76 WGRVVFTLLWLWLLLGVVSWRVW
Inside 77-445 RKMQQLKAERQHEVVLEQDPVKGLIDRQALFLDRWLQALNTHLGKGALYAMPWYLVLGLPGSGKSSLIHRANPANKLNPRLDTELRDVAQDQLVDCWLGEQAVMLDPAGVLLSQSEAELDPQARKHERLWLHLLGWLNEHRRRQPLNGLVLTVDLAWLSHASVAERKAYAQLMRSRLQEVSATMNTRLPLYVTFTKLDLLRGFDVIYQQLDKEAREAVLGVTFKPGADWQQDLALFWDQWVDNLNQNLPELMLSRLDAAQRNALFSFVRQLAGLKDYVTSLLAETLAIEESKPLLVRGVYVSSVYQQGVPFDAFAQAASRRYNLPEPIHSALRGESNTYFVRQLFSSIIFPEAHLAGENRLHTLYRRRR
Transmembrane helix 446-468 MAIGLSCLSLFSAALIGGWHYFY
Outside 469-1161 RVNEEAGRNVLTKAQAFMETNEVADAHAFGVSQLPRLNLIREATLSFGNYRERMPLVADLGLYQGDEIGPYVEGSYLQLLSLRFLPAQMQGLLEDLNQAPAGSEEKLAILRVMRMLDDASGRNKELVAQYMASRWQKAFPGQGAVQEQLMGHLDYALDHTNWYGARAERDQAAITAFVPFKEPVYGAQRELGKLPMYQRVYQNLVVKANDVLPPDLNVRDEVGPTFDTVFALRSDNAGQVPRLLTWPGFNDFFLKQDKALIDLTAMDAWVLGQRKLSQLSEADRKEITRQVNDRYVTDYVNQWQKLLTNLDVQTLESPEQALDVLAAITGNDQPFQRVLASLDDNTRIRKISDVEGDPAQAISARIGRPFMATNGVLAGRGEQGPLIQEVNQKLVELQHYLELIVNATEPGQSALKAVQLRMTNKYADPVFALQQYARSLPAPLDRWVGQLSEQSSRLVIDLAMSSLNQEWQDKVLAPFNSQLAGRYPFDPSSNKDVPLSEMERFFAPGGTLDSFYQVNLKPMVESGLMEGEFSSPIQAELVKQLDRAARIRQIFFSQQGNLEVQFALEPIELTANKRRSVLNLDGQLLEYAHGRRTKIPLVWPNTMRDGAESKITLVPAARERSPRSEGFVGPWAMFRLMDKGELTQVNDATFDVRFPVDQGAMTYRVYTDSAQNPFTGGLFSQFRLPESLY


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9941 - -


  Protein sequence: 1162 a.a.    

>T6CP010922 NZ_JH815591:1045005-1048490 [Aeromonas dhakensis] [TssM]
MFKTIFTFLRQQLPKLKPSWPLLGAVLWVLALILVWWLGPRLELRGAKPFEPLWGRVVFTLLWLWLLLGVVSWRVWRKMQ
QLKAERQHEVVLEQDPVKGLIDRQALFLDRWLQALNTHLGKGALYAMPWYLVLGLPGSGKSSLIHRANPANKLNPRLDTE
LRDVAQDQLVDCWLGEQAVMLDPAGVLLSQSEAELDPQARKHERLWLHLLGWLNEHRRRQPLNGLVLTVDLAWLSHASVA
ERKAYAQLMRSRLQEVSATMNTRLPLYVTFTKLDLLRGFDVIYQQLDKEAREAVLGVTFKPGADWQQDLALFWDQWVDNL
NQNLPELMLSRLDAAQRNALFSFVRQLAGLKDYVTSLLAETLAIEESKPLLVRGVYVSSVYQQGVPFDAFAQAASRRYNL
PEPIHSALRGESNTYFVRQLFSSIIFPEAHLAGENRLHTLYRRRRMAIGLSCLSLFSAALIGGWHYFYRVNEEAGRNVLT
KAQAFMETNEVADAHAFGVSQLPRLNLIREATLSFGNYRERMPLVADLGLYQGDEIGPYVEGSYLQLLSLRFLPAQMQGL
LEDLNQAPAGSEEKLAILRVMRMLDDASGRNKELVAQYMASRWQKAFPGQGAVQEQLMGHLDYALDHTNWYGARAERDQA
AITAFVPFKEPVYGAQRELGKLPMYQRVYQNLVVKANDVLPPDLNVRDEVGPTFDTVFALRSDNAGQVPRLLTWPGFNDF
FLKQDKALIDLTAMDAWVLGQRKLSQLSEADRKEITRQVNDRYVTDYVNQWQKLLTNLDVQTLESPEQALDVLAAITGND
QPFQRVLASLDDNTRIRKISDVEGDPAQAISARIGRPFMATNGVLAGRGEQGPLIQEVNQKLVELQHYLELIVNATEPGQ
SALKAVQLRMTNKYADPVFALQQYARSLPAPLDRWVGQLSEQSSRLVIDLAMSSLNQEWQDKVLAPFNSQLAGRYPFDPS
SNKDVPLSEMERFFAPGGTLDSFYQVNLKPMVESGLMEGEFSSPIQAELVKQLDRAARIRQIFFSQQGNLEVQFALEPIE
LTANKRRSVLNLDGQLLEYAHGRRTKIPLVWPNTMRDGAESKITLVPAARERSPRSEGFVGPWAMFRLMDKGELTQVNDA
TFDVRFPVDQGAMTYRVYTDSAQNPFTGGLFSQFRLPESLY*

  Nucleotide sequence: 3486 bp    

>T6CP010922 NZ_JH815591:1045005-1048490 [Aeromonas dhakensis] [TssM]
ATGTTCAAAACCATTTTTACTTTTCTGCGGCAACAGCTGCCGAAATTGAAACCCTCCTGGCCCCTGCTCGGTGCAGTGCT
CTGGGTGCTCGCCCTCATCCTGGTGTGGTGGCTGGGCCCTCGTCTTGAGCTGCGCGGCGCCAAGCCGTTCGAGCCGCTGT
GGGGGCGGGTGGTGTTCACCCTGCTCTGGCTCTGGCTGCTGCTGGGAGTAGTCTCCTGGCGGGTATGGCGCAAGATGCAG
CAGCTCAAGGCCGAGCGTCAGCACGAGGTGGTGCTGGAGCAGGATCCGGTCAAGGGGCTCATCGACAGGCAGGCGCTGTT
CCTCGATCGCTGGCTGCAGGCGCTGAATACCCATCTGGGCAAGGGGGCGCTCTACGCCATGCCCTGGTATTTGGTGCTGG
GGCTGCCCGGCAGCGGCAAGAGCAGCCTCATTCACCGCGCCAACCCGGCCAACAAGCTCAACCCGAGACTCGATACCGAA
CTGCGCGACGTGGCGCAGGATCAGCTGGTGGATTGCTGGCTGGGGGAACAGGCGGTGATGCTGGATCCGGCCGGGGTACT
GCTCTCCCAGAGCGAAGCCGAACTGGATCCGCAGGCCCGCAAACATGAGCGGCTCTGGCTGCACCTGCTTGGCTGGCTCA
ATGAACATCGCCGTCGCCAGCCCCTCAACGGCCTGGTGCTGACCGTGGATCTGGCCTGGCTCTCCCACGCCAGCGTGGCT
GAACGCAAGGCTTATGCCCAGCTGATGCGCTCGCGCCTGCAGGAGGTGTCGGCCACCATGAATACCCGGTTGCCGCTCTA
TGTCACCTTCACCAAGCTCGATCTGCTGCGCGGCTTTGACGTGATCTACCAGCAGCTCGACAAGGAGGCCCGCGAGGCCG
TGCTGGGGGTGACCTTCAAGCCGGGCGCCGACTGGCAGCAGGATCTGGCCCTGTTCTGGGATCAGTGGGTGGACAACCTC
AACCAGAATCTGCCCGAACTGATGCTGAGCCGGCTCGATGCCGCCCAGCGCAACGCGCTGTTCTCGTTCGTGCGCCAGCT
GGCGGGACTCAAGGATTACGTGACCAGCCTGCTGGCCGAGACCCTGGCCATCGAGGAGAGCAAGCCGCTCTTGGTACGCG
GGGTTTATGTTAGCTCCGTCTATCAGCAGGGGGTACCGTTCGATGCGTTTGCCCAGGCAGCCTCCCGCCGCTACAACCTG
CCAGAGCCCATCCACTCGGCCCTGCGTGGGGAGTCGAACACCTATTTCGTGCGCCAGCTGTTCTCCTCCATCATCTTCCC
GGAGGCCCATCTGGCCGGCGAAAACCGGCTGCACACCCTCTATCGCCGTCGCCGCATGGCCATCGGCCTCAGCTGCCTGT
CGCTCTTCTCGGCAGCCCTCATCGGTGGCTGGCACTACTTCTACCGGGTCAACGAGGAGGCGGGCCGCAACGTATTGACC
AAGGCCCAGGCCTTCATGGAGACCAACGAGGTGGCCGACGCCCACGCCTTCGGCGTGAGCCAGCTGCCGCGCCTCAACCT
CATTCGCGAGGCGACCCTCTCCTTTGGTAACTACCGCGAGCGCATGCCACTGGTGGCCGACCTCGGCCTCTACCAGGGGG
ACGAAATTGGTCCCTATGTGGAGGGTTCCTACCTGCAACTGCTGAGCCTGCGCTTCCTGCCGGCCCAGATGCAGGGGTTG
CTTGAGGATCTCAACCAGGCGCCTGCGGGGAGTGAGGAGAAGCTTGCCATCCTGCGGGTGATGCGGATGCTGGACGATGC
GTCCGGTCGCAACAAGGAGCTGGTGGCGCAGTACATGGCGAGCCGCTGGCAGAAGGCCTTCCCGGGGCAGGGGGCGGTAC
AGGAGCAACTGATGGGGCACCTGGACTATGCCCTCGATCACACCAACTGGTATGGCGCCAGGGCAGAGCGGGATCAGGCC
GCCATCACCGCCTTCGTGCCCTTCAAGGAGCCGGTCTACGGTGCCCAGCGCGAGCTCGGCAAGCTGCCCATGTATCAGCG
GGTCTACCAGAACCTGGTGGTGAAGGCGAACGACGTGCTGCCGCCGGATCTCAACGTGCGTGACGAGGTGGGCCCCACCT
TCGACACCGTGTTTGCCCTGCGCAGCGACAACGCCGGCCAGGTGCCGCGGCTGCTGACCTGGCCCGGTTTCAACGACTTC
TTCCTCAAGCAAGACAAGGCCCTCATCGATCTCACCGCCATGGATGCCTGGGTGCTGGGCCAGCGCAAGCTGAGCCAGCT
GAGCGAGGCGGATCGCAAGGAGATCACCCGTCAGGTCAACGACCGTTACGTCACCGATTACGTCAACCAGTGGCAGAAAC
TGCTGACCAATCTGGATGTGCAGACCCTGGAGAGCCCGGAGCAGGCGCTGGACGTGCTCGCTGCCATTACCGGCAACGAT
CAGCCGTTCCAGCGGGTACTGGCCTCGCTGGATGACAACACCCGCATTCGCAAGATCTCCGATGTGGAAGGGGACCCGGC
CCAGGCCATCAGCGCCCGCATCGGTCGCCCCTTTATGGCCACTAATGGAGTATTGGCTGGACGTGGTGAGCAGGGGCCGC
TCATTCAGGAGGTCAACCAGAAGCTGGTGGAGCTGCAGCACTACCTGGAGCTCATCGTCAACGCCACCGAGCCGGGCCAG
TCTGCCTTGAAAGCGGTGCAGCTGCGCATGACCAACAAGTACGCCGATCCGGTGTTCGCGCTGCAGCAATATGCCCGCAG
CCTGCCGGCCCCGCTCGATCGCTGGGTGGGTCAGCTCTCCGAGCAGAGCTCGCGGCTGGTGATCGACCTGGCCATGTCCT
CCCTCAACCAGGAGTGGCAGGACAAGGTGCTGGCGCCCTTCAACAGCCAGCTGGCCGGCCGTTATCCGTTTGATCCGAGC
TCGAACAAGGACGTGCCCCTCTCCGAGATGGAGCGCTTCTTCGCGCCGGGCGGGACGCTGGACAGCTTCTATCAGGTCAA
CCTCAAACCCATGGTGGAGAGCGGCCTGATGGAAGGGGAGTTCAGTTCGCCCATCCAGGCCGAGCTGGTCAAACAGCTGG
ACCGTGCTGCCCGCATCCGTCAGATCTTCTTCAGCCAGCAGGGCAACCTGGAGGTGCAGTTCGCCCTCGAGCCCATCGAG
CTCACCGCCAACAAGCGGCGCAGCGTGCTGAACCTGGATGGTCAGCTGTTGGAATACGCCCATGGCCGCCGTACCAAGAT
CCCGCTGGTGTGGCCCAACACCATGCGCGACGGGGCCGAGAGCAAGATCACCCTGGTGCCTGCCGCCCGCGAGCGTTCAC
CGCGCAGTGAAGGCTTCGTCGGCCCCTGGGCCATGTTCCGCCTGATGGACAAGGGGGAGCTGACCCAGGTCAATGACGCC
ACCTTCGATGTGCGCTTCCCGGTGGATCAGGGGGCCATGACCTATCGCGTCTACACCGACAGCGCGCAAAACCCCTTCAC
CGGCGGTCTGTTCAGCCAGTTCAGACTGCCCGAATCCCTCTATTGA