Detailed information of immunity protein
Summary
| Immunity protein ID | IMU17302  |
| Cognate effector | EFF01888 |
| Related T6SS (Type) | T6SS00788 (Type i1) |
| Strain | Aeromonas dhakensis strain SSU |
| Replicon | chromosome |
| Sequence | Protein sequence (163 a.a.); Nucleotide sequence (489 bp) |
| Description | Complementation with a plasmid-borne tsiP fully restored survival of ΔtsePei mutant which was efficiently outcompeted by the wild type but not by the T6SS-null ΔvasK mutant, the DtseP mutant, or the chromosomal E655A and E663A TseP mutants. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | HMPREF1171_RS23530 |
| Coordinates (Strand) | 1055395..1055883 (+) |
| NCBI ID | WP_154956507.1 |
| RefSeq | NZ_JH815591 |
| Uniprot ID | UPI0012B82CFA |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-6 | MKRYDF |
| Transmembrane helix | 7-24 | LFFVFLFFSFFVNAADII |
| Outside | 25-162 | QGPYRISNDTASVKIEKNDDINCPLSMVIYDKSSHYELDKICENGDYPNLRSVFFFTLKGVNHIGTIVSWHNKHQAEGIDETNFEVKIYKKNAGGEYTVDKEKTLDPVLSGTEDGAGDGTYKFNNAISVKKYMKERYG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9857 | pos: 20-21. VNA-AD. | MKRYDFLFFVFLFFSFFVNA |
Protein sequence: 163 a.a. .
>IMU17302 NZ_JH815591:1055395-1055883 [Aeromonas dhakensis]
MKRYDFLFFVFLFFSFFVNAADIIQGPYRISNDTASVKIEKNDDINCPLSMVIYDKSSHYELDKICENGDYPNLRSVFFF
TLKGVNHIGTIVSWHNKHQAEGIDETNFEVKIYKKNAGGEYTVDKEKTLDPVLSGTEDGAGDGTYKFNNAISVKKYMKER
YG*
MKRYDFLFFVFLFFSFFVNAADIIQGPYRISNDTASVKIEKNDDINCPLSMVIYDKSSHYELDKICENGDYPNLRSVFFF
TLKGVNHIGTIVSWHNKHQAEGIDETNFEVKIYKKNAGGEYTVDKEKTLDPVLSGTEDGAGDGTYKFNNAISVKKYMKER
YG*
Nucleotide sequence: 489 bp .
>IMU17302 NZ_JH815591:1055395-1055883 [Aeromonas dhakensis]
ATGAAAAGATATGATTTTCTATTTTTTGTTTTTCTGTTTTTTTCTTTCTTTGTGAATGCGGCTGATATTATTCAAGGGCC
CTATAGGATTAGTAATGATACAGCGAGCGTTAAAATTGAGAAAAATGATGACATAAACTGCCCATTATCTATGGTCATAT
ATGATAAATCATCACACTATGAGTTGGATAAAATATGTGAGAACGGCGATTACCCGAATCTCAGGAGCGTTTTTTTCTTT
ACTCTGAAAGGTGTGAATCATATAGGAACTATAGTTTCATGGCATAATAAGCATCAGGCAGAAGGGATTGATGAGACAAA
TTTTGAAGTTAAAATTTATAAAAAGAACGCTGGTGGAGAATATACTGTTGATAAGGAAAAGACTTTGGATCCAGTTTTAA
GTGGCACAGAGGATGGGGCCGGTGATGGAACATATAAGTTTAACAATGCAATATCGGTAAAGAAGTATATGAAAGAAAGA
TATGGTTAA
ATGAAAAGATATGATTTTCTATTTTTTGTTTTTCTGTTTTTTTCTTTCTTTGTGAATGCGGCTGATATTATTCAAGGGCC
CTATAGGATTAGTAATGATACAGCGAGCGTTAAAATTGAGAAAAATGATGACATAAACTGCCCATTATCTATGGTCATAT
ATGATAAATCATCACACTATGAGTTGGATAAAATATGTGAGAACGGCGATTACCCGAATCTCAGGAGCGTTTTTTTCTTT
ACTCTGAAAGGTGTGAATCATATAGGAACTATAGTTTCATGGCATAATAAGCATCAGGCAGAAGGGATTGATGAGACAAA
TTTTGAAGTTAAAATTTATAAAAAGAACGCTGGTGGAGAATATACTGTTGATAAGGAAAAGACTTTGGATCCAGTTTTAA
GTGGCACAGAGGATGGGGCCGGTGATGGAACATATAAGTTTAACAATGCAATATCGGTAAAGAAGTATATGAAAGAAAGA
TATGGTTAA