Detailed information of effector
Summary
| Effector ID | EFF01888  |
| Effector type | Tpe |
| Cognate immunity protein | IMU17302 |
| Related T6SS (Type) | T6SS00788 (Type i1) |
| Strain | Aeromonas dhakensis strain SSU |
| Replicon | chromosome |
| Sequence | Protein sequence (208 a.a.); Nucleotide sequence (624 bp) |
| Description | Wild-type TseP reduced the optical density of cell wall to the baseline level within 5 min of treatment while TsePE663A had no effect on optical density showed evidence of the lysozyme-like activity of TseP cleaving the peptidoglycan chain. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | HMPREF1171_RS04800 (TseP) |
| Coordinates (Strand) | 1054604..1055227 (+) |
| NCBI ID | WP_029305891.1 |
| RefSeq | NZ_JH815591 |
| Uniprot ID | UPI0004932A7D |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-207 | MKEERAMAHFLSQVGHESNFKPVSENLRYSPKGMRKIFGCKGGSKNYDPILDDAKEGRLRPKLWTHESDYAFNPVALGNYVYANRPGSKNGDESSGDGYKYRGRGLIQITHKDAYIKFTEAHNAANPSDQKDFLASPDDILTLRYATSSAYFFWFIYKKSFNLHSTACTGTVKEVTKIVNGGYAGYADRLKRFNAVAAVIGIDGARE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9613 | - | - |
Protein sequence: 208 a.a. .
>EFF01888 NZ_JH815591:1054604-1055227 Type VI Peptidase Effector (Tpe) [Aeromonas dhakensis]
MKEERAMAHFLSQVGHESNFKPVSENLRYSPKGMRKIFGCKGGSKNYDPILDDAKEGRLRPKLWTHESDYAFNPVALGNY
VYANRPGSKNGDESSGDGYKYRGRGLIQITHKDAYIKFTEAHNAANPSDQKDFLASPDDILTLRYATSSAYFFWFIYKKS
FNLHSTACTGTVKEVTKIVNGGYAGYADRLKRFNAVAAVIGIDGARE*
MKEERAMAHFLSQVGHESNFKPVSENLRYSPKGMRKIFGCKGGSKNYDPILDDAKEGRLRPKLWTHESDYAFNPVALGNY
VYANRPGSKNGDESSGDGYKYRGRGLIQITHKDAYIKFTEAHNAANPSDQKDFLASPDDILTLRYATSSAYFFWFIYKKS
FNLHSTACTGTVKEVTKIVNGGYAGYADRLKRFNAVAAVIGIDGARE*
Nucleotide sequence: 624 bp .
>EFF01888 NZ_JH815591:1054604-1055227 Type VI Peptidase Effector (Tpe) [Aeromonas dhakensis]
ATGAAAGAAGAAAGAGCCATGGCCCATTTCTTATCCCAAGTAGGTCATGAGTCAAACTTTAAACCTGTAAGTGAGAATCT
TCGTTATAGTCCTAAAGGTATGCGGAAAATTTTTGGTTGTAAAGGGGGATCTAAAAACTACGATCCTATATTAGATGATG
CAAAGGAGGGAAGGTTAAGACCGAAGTTGTGGACTCATGAGAGCGATTATGCATTCAATCCTGTGGCTTTAGGAAACTAT
GTCTACGCCAACAGACCGGGAAGTAAAAACGGCGATGAGAGCTCTGGTGATGGATATAAATATCGAGGCAGAGGGTTGAT
TCAAATAACACACAAGGATGCATACATAAAATTCACCGAAGCTCATAATGCAGCAAACCCCTCAGACCAAAAAGACTTTT
TGGCTTCACCGGATGACATACTGACACTTCGATATGCTACATCATCTGCATACTTTTTTTGGTTTATATACAAAAAAAGC
TTTAATTTGCATTCAACTGCATGCACAGGGACAGTCAAAGAGGTTACAAAAATTGTAAATGGAGGCTATGCTGGTTATGC
AGATAGATTAAAGAGGTTTAATGCGGTTGCTGCAGTTATTGGTATTGATGGGGCGAGAGAGTGA
ATGAAAGAAGAAAGAGCCATGGCCCATTTCTTATCCCAAGTAGGTCATGAGTCAAACTTTAAACCTGTAAGTGAGAATCT
TCGTTATAGTCCTAAAGGTATGCGGAAAATTTTTGGTTGTAAAGGGGGATCTAAAAACTACGATCCTATATTAGATGATG
CAAAGGAGGGAAGGTTAAGACCGAAGTTGTGGACTCATGAGAGCGATTATGCATTCAATCCTGTGGCTTTAGGAAACTAT
GTCTACGCCAACAGACCGGGAAGTAAAAACGGCGATGAGAGCTCTGGTGATGGATATAAATATCGAGGCAGAGGGTTGAT
TCAAATAACACACAAGGATGCATACATAAAATTCACCGAAGCTCATAATGCAGCAAACCCCTCAGACCAAAAAGACTTTT
TGGCTTCACCGGATGACATACTGACACTTCGATATGCTACATCATCTGCATACTTTTTTTGGTTTATATACAAAAAAAGC
TTTAATTTGCATTCAACTGCATGCACAGGGACAGTCAAAGAGGTTACAAAAATTGTAAATGGAGGCTATGCTGGTTATGC
AGATAGATTAAAGAGGTTTAATGCGGTTGCTGCAGTTATTGGTATTGATGGGGCGAGAGAGTGA