Detailed information of component protein


Summary

Component ID T6CP010923
Component protein type TssA
T6SS ID (Type) T6SS00788 experimental (Type i1)
Strain Aeromonas dhakensis strain SSU
Replicon chromosome
Sequence Protein sequence (477 a.a.); Nucleotide sequence (1431 bp)
Reference
[1] Suarez G, Sierra JC, Sha J, et al. Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog. 2008 Apr;44(4):344-61. Epub 2007 Oct 24. PMID: 18037263
[2] Suarez G, Sierra JC, Erova TE, et al. A type VI secretion system effector protein, VgrG1, from Aeromonas hydrophila that induces host cell toxicity by ADP ribosylation of actin. J Bacteriol. 2010 Jan;192(1):155-68. doi: 10.1128/JB.01260-09. PMID: 19880608
[3] Sha J, Rosenzweig JA, Kozlova EV, et al. Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis. Microbiology (Reading). 2013 Jun;159(Pt 6):1120-1135. doi: 10.1099/mic.0.063495-0. Epub 2013 Mar 21. PMID: 23519162
[4] Suarez G, Sierra JC, Kirtley ML, et al. Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. Microbiology (Reading). 2010 Dec;156(Pt 12):3678-3688. doi: 10.1099/mic.0.041277-0. Epub 2010 Aug 26. PMID: 20798163
[5] Grim CJ, Kozlova EV, Ponnusamy D, et al. Functional genomic characterization of virulence factors from necrotizing fasciitis-causing strains of Aeromonas hydrophila. Appl Environ Microbiol. 2014 Jul;80(14):4162-83. doi: 10.1128/AEM.00486-14. Epub 2014 May 2. PMID: 24795370
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) HMPREF1171_RS04780
Coordinate (Strand) 1048537..1049967 (+)
NCBI ID WP_005300006.1
RefSeq NZ_JH815591
Uniprot ID K1JQV1_9GAMM
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VasL PF12486.10 1.8e-53 324..470
ImpA_N PF06812.14 5.8e-23 10..115
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-249 MSQNQQGQQALKVGRDPRMLPEYEALRAEINKLSHASRPEVDWQRIHQLASLIFEKHGVDLQTAIYFTLARSRLQGLSGFTEGCEFLANLIVTQWESFWPPVHQERARIEMLDWFIARISDVIRQYQISHEDKRLIYRCERALQLISEKLHNADLSRIPRVENLVHFIEGYTHLFDETEIVIVSDEPGLKEDLQIPPMVFFKSDMESDHGGTVSASTPHLPQGSILVGREKGQVKPTVLKIEQHRRQRP
Transmembrane helix 250-272 AWFWFGCGLLSCALPVMGWLGWQ
Outside 273-476 QHQQEKTVAAQRLVQPAAELPRALSYDDIRQARIVLGEQTLQNMESDLVARYQNQLTRLEQTSPLYWYRYGEGLRNSLQMLYPDSLAVKALDKQWQTQLGSQQGDVISVPTYLDARAGVDALLDQLLELERQRKTVTISYLKSQLYEVQKNLMQNIPFSLRLDELEARKASQEPITAAELKSLENDLKALNIRLYQLQQGASGS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9958 - -


  Protein sequence: 477 a.a.    

>T6CP010923 NZ_JH815591:1048537-1049967 [Aeromonas dhakensis] [TssA]
MSQNQQGQQALKVGRDPRMLPEYEALRAEINKLSHASRPEVDWQRIHQLASLIFEKHGVDLQTAIYFTLARSRLQGLSGF
TEGCEFLANLIVTQWESFWPPVHQERARIEMLDWFIARISDVIRQYQISHEDKRLIYRCERALQLISEKLHNADLSRIPR
VENLVHFIEGYTHLFDETEIVIVSDEPGLKEDLQIPPMVFFKSDMESDHGGTVSASTPHLPQGSILVGREKGQVKPTVLK
IEQHRRQRPAWFWFGCGLLSCALPVMGWLGWQQHQQEKTVAAQRLVQPAAELPRALSYDDIRQARIVLGEQTLQNMESDL
VARYQNQLTRLEQTSPLYWYRYGEGLRNSLQMLYPDSLAVKALDKQWQTQLGSQQGDVISVPTYLDARAGVDALLDQLLE
LERQRKTVTISYLKSQLYEVQKNLMQNIPFSLRLDELEARKASQEPITAAELKSLENDLKALNIRLYQLQQGASGS*

  Nucleotide sequence: 1431 bp    

>T6CP010923 NZ_JH815591:1048537-1049967 [Aeromonas dhakensis] [TssA]
ATGTCACAGAATCAACAAGGTCAGCAGGCCCTGAAAGTGGGGCGCGACCCCAGGATGTTGCCGGAGTACGAGGCGCTGCG
GGCCGAGATCAACAAGCTCAGCCACGCTTCGCGCCCCGAGGTAGACTGGCAGCGGATCCATCAGCTCGCCAGCCTCATCT
TCGAAAAACACGGGGTGGATCTGCAGACCGCCATCTACTTCACCCTGGCCCGTTCGCGCCTGCAGGGGCTGAGCGGTTTT
ACCGAAGGGTGCGAGTTTCTCGCCAACCTCATCGTCACCCAGTGGGAGAGCTTCTGGCCGCCGGTCCATCAGGAACGAGC
CCGTATCGAGATGCTCGACTGGTTCATCGCCCGCATCAGCGACGTCATTCGCCAGTACCAGATCAGCCACGAGGACAAGC
GGCTGATCTATCGCTGCGAGCGGGCGCTGCAGCTCATCAGCGAGAAGCTGCACAACGCGGATCTGAGCCGCATTCCCCGG
GTCGAGAATCTGGTGCACTTCATCGAGGGGTATACCCACCTGTTCGATGAGACCGAGATCGTCATCGTCTCCGACGAGCC
GGGCCTCAAGGAGGATCTGCAGATCCCGCCCATGGTGTTCTTCAAGTCCGACATGGAGAGTGATCACGGCGGCACGGTTT
CGGCATCGACCCCGCACCTGCCCCAGGGCAGCATTCTGGTGGGGCGCGAGAAGGGGCAGGTCAAGCCCACCGTGCTGAAG
ATTGAGCAGCACCGCCGGCAGCGTCCCGCCTGGTTCTGGTTTGGCTGCGGCCTGCTCAGCTGCGCCCTGCCGGTGATGGG
GTGGCTCGGCTGGCAGCAACATCAGCAGGAGAAAACCGTGGCGGCCCAGCGCCTGGTACAGCCGGCCGCCGAGCTGCCCC
GAGCCCTCAGCTACGACGACATTCGCCAGGCACGCATCGTGCTGGGGGAGCAAACTCTGCAGAACATGGAGAGCGATCTG
GTGGCCCGCTACCAGAACCAGTTGACCCGGCTCGAGCAGACGTCGCCGCTCTACTGGTATCGCTACGGCGAGGGACTGCG
GAACTCGTTACAGATGCTCTATCCCGACTCGCTGGCGGTCAAGGCGCTGGACAAGCAGTGGCAGACGCAGCTTGGCAGTC
AGCAGGGGGATGTCATCAGCGTGCCCACCTATCTGGATGCCCGCGCCGGGGTGGATGCCCTGCTTGATCAGCTGCTGGAG
CTGGAGCGCCAGCGCAAGACGGTGACCATTTCCTACCTCAAATCCCAGCTCTACGAAGTGCAGAAGAACCTGATGCAGAA
CATCCCGTTCAGTCTGCGCCTGGATGAACTGGAGGCGAGAAAAGCCTCTCAGGAGCCCATCACGGCAGCGGAGCTCAAGA
GCCTGGAAAATGATCTGAAAGCGCTCAACATCCGCCTCTATCAGCTGCAACAGGGCGCTTCTGGTAGCTGA