Detailed information of component protein


Summary

Component ID T6CP010920
Component protein type TssH
T6SS ID (Type) T6SS00788 experimental (Type i1)
Strain Aeromonas dhakensis strain SSU
Replicon chromosome
Sequence Protein sequence (881 a.a.); Nucleotide sequence (2643 bp)
Reference
[1] Suarez G, Sierra JC, Sha J, et al. Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog. 2008 Apr;44(4):344-61. Epub 2007 Oct 24. PMID: 18037263
[2] Suarez G, Sierra JC, Erova TE, et al. A type VI secretion system effector protein, VgrG1, from Aeromonas hydrophila that induces host cell toxicity by ADP ribosylation of actin. J Bacteriol. 2010 Jan;192(1):155-68. doi: 10.1128/JB.01260-09. PMID: 19880608
[3] Sha J, Rosenzweig JA, Kozlova EV, et al. Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis. Microbiology (Reading). 2013 Jun;159(Pt 6):1120-1135. doi: 10.1099/mic.0.063495-0. Epub 2013 Mar 21. PMID: 23519162
[4] Suarez G, Sierra JC, Kirtley ML, et al. Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. Microbiology (Reading). 2010 Dec;156(Pt 12):3678-3688. doi: 10.1099/mic.0.041277-0. Epub 2010 Aug 26. PMID: 20798163
[5] Grim CJ, Kozlova EV, Ponnusamy D, et al. Functional genomic characterization of virulence factors from necrotizing fasciitis-causing strains of Aeromonas hydrophila. Appl Environ Microbiol. 2014 Jul;80(14):4162-83. doi: 10.1128/AEM.00486-14. Epub 2014 May 2. PMID: 24795370
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) HMPREF1171_RS04755
Coordinate (Strand) 1038721..1041363 (+)
NCBI ID WP_005299997.1
RefSeq NZ_JH815591
Uniprot ID K1JQU7_9GAMM
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 6.7e-37 617..778
AAA_lid_9 PF17871.3 1.4e-28 362..453
ClpB_D2-small PF10431.11 3.1e-10 784..858
AAA PF00004.31 5.8e-08 222..317
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-880 MIRIELPVLVERLNPICRHMLEEAAALCVNHQGAEIRIEHLLLKMLETPLSDVRQILKVAEVDVEELKALLQPSPADNGYGQGYPSFSPMLVEWLQDSWLLASAELQHAQLRSGVMLLVLLMTPQRYLPGSVTRLLAKVNRELLRQQFDEWVKESAETQVTTTPGGKTAQAALPADASLLARFTVNVTEQARQGSLDPVLCRDHEIDLMIDILSRRRKNNPIVVGEAGVGKSALIEGLALRMVAGQVPEKLRGVELMTLDLGAMQAGASVKGEFEKRFKGVMQEVKDAVQPVILFIDEAHTLIGAGNQAGGLDVSNLIKPALARGELRTIAATTWGEYKKYVEKDAALSRRFQLVKVGEPDADEATVILRGLRSIYEKAHGVLIDEEALQASAQLSARYISGRQLPDKAIDVLDTACARVAINLTTPPRAVSHLQNALRQRELEIRQLERQSLIGLGDNAERLAELQTAQQACREELAEQEARWQQQQGLVHQIVELRAALLADQQDEMLAREALDLADAPLDPQAAAEQLATLERELAELQQGEVLVSAHVDKTQIAAVIAEWTGVPLNRISQGELDVVTRLPEYLGELIKGQDVAVAHLHKHLLTARADLRRPGRPLGAFLLVGPSGVGKTETVLQIAELMFGGRQYLTTINMSEYQEKHTVSRLIGSPPGYVGFGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAFDKGELADGEGRIIDCKNVVFFLTSNLGFQTIVDHAEQPDVLLDALYPELAAFFKPALLARMEVIPYLPLGHDTLVQIVGGKLNRLVKLLKERFGAEVVLDDEVAEEILLRANRSENGARMLESVIDGALLPPVSLQLLQRLSAGEPIKRVHFSVADHQFVAEVGA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9980 - -


  Protein sequence: 881 a.a.    

>T6CP010920 NZ_JH815591:1038721-1041363 [Aeromonas dhakensis] [TssH]
MIRIELPVLVERLNPICRHMLEEAAALCVNHQGAEIRIEHLLLKMLETPLSDVRQILKVAEVDVEELKALLQPSPADNGY
GQGYPSFSPMLVEWLQDSWLLASAELQHAQLRSGVMLLVLLMTPQRYLPGSVTRLLAKVNRELLRQQFDEWVKESAETQV
TTTPGGKTAQAALPADASLLARFTVNVTEQARQGSLDPVLCRDHEIDLMIDILSRRRKNNPIVVGEAGVGKSALIEGLAL
RMVAGQVPEKLRGVELMTLDLGAMQAGASVKGEFEKRFKGVMQEVKDAVQPVILFIDEAHTLIGAGNQAGGLDVSNLIKP
ALARGELRTIAATTWGEYKKYVEKDAALSRRFQLVKVGEPDADEATVILRGLRSIYEKAHGVLIDEEALQASAQLSARYI
SGRQLPDKAIDVLDTACARVAINLTTPPRAVSHLQNALRQRELEIRQLERQSLIGLGDNAERLAELQTAQQACREELAEQ
EARWQQQQGLVHQIVELRAALLADQQDEMLAREALDLADAPLDPQAAAEQLATLERELAELQQGEVLVSAHVDKTQIAAV
IAEWTGVPLNRISQGELDVVTRLPEYLGELIKGQDVAVAHLHKHLLTARADLRRPGRPLGAFLLVGPSGVGKTETVLQIA
ELMFGGRQYLTTINMSEYQEKHTVSRLIGSPPGYVGFGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAFDKGEL
ADGEGRIIDCKNVVFFLTSNLGFQTIVDHAEQPDVLLDALYPELAAFFKPALLARMEVIPYLPLGHDTLVQIVGGKLNRL
VKLLKERFGAEVVLDDEVAEEILLRANRSENGARMLESVIDGALLPPVSLQLLQRLSAGEPIKRVHFSVADHQFVAEVGA
*

  Nucleotide sequence: 2643 bp    

>T6CP010920 NZ_JH815591:1038721-1041363 [Aeromonas dhakensis] [TssH]
TTGATTCGTATAGAGCTACCGGTACTGGTGGAAAGACTCAACCCCATCTGCCGTCACATGCTGGAGGAGGCCGCGGCCCT
CTGTGTCAATCACCAGGGGGCCGAGATCCGCATCGAGCACCTGCTGCTCAAGATGCTGGAGACCCCGCTGAGCGACGTGC
GCCAGATCCTGAAGGTGGCCGAGGTCGACGTTGAAGAGCTGAAGGCCCTGCTGCAACCCTCGCCAGCTGACAACGGTTAT
GGCCAGGGTTACCCCTCCTTCTCGCCCATGCTGGTGGAGTGGCTGCAAGACAGCTGGCTGCTCGCCTCCGCCGAGCTGCA
ACACGCCCAGCTGCGCAGCGGTGTCATGCTGCTGGTGCTGCTGATGACCCCCCAGCGCTATCTGCCGGGCTCGGTGACCC
GCCTGCTTGCCAAGGTCAATCGCGAGCTGCTGCGCCAGCAGTTTGACGAGTGGGTCAAGGAGTCTGCCGAGACACAAGTG
ACCACAACCCCCGGCGGCAAGACGGCTCAGGCCGCGCTGCCGGCCGATGCCAGCCTGCTGGCCCGCTTCACCGTCAATGT
GACCGAACAGGCCCGCCAGGGCAGCCTGGATCCCGTGCTCTGCCGGGATCACGAGATCGACCTGATGATCGACATCCTCT
CCCGTCGTCGCAAGAACAACCCCATAGTCGTGGGGGAGGCTGGTGTCGGCAAGAGCGCCCTCATCGAGGGGCTGGCTCTG
CGCATGGTGGCGGGCCAGGTGCCCGAGAAGCTGCGCGGGGTCGAGCTGATGACGCTGGATCTCGGTGCCATGCAGGCCGG
CGCTTCGGTCAAGGGGGAGTTCGAGAAACGCTTTAAGGGGGTGATGCAGGAGGTCAAGGACGCCGTGCAGCCGGTCATCC
TCTTCATCGACGAGGCGCACACCCTGATCGGGGCCGGCAACCAGGCCGGCGGGCTGGATGTCTCCAACCTCATCAAGCCG
GCGCTGGCCCGGGGCGAGCTGCGCACCATCGCCGCCACCACCTGGGGCGAGTACAAGAAATACGTTGAAAAAGACGCGGC
CCTGTCGCGCCGCTTCCAGCTGGTGAAAGTCGGTGAGCCCGATGCCGACGAGGCCACCGTGATCCTGCGCGGCCTGCGCA
GCATCTACGAGAAGGCCCACGGGGTGCTGATCGACGAGGAGGCGCTGCAGGCCTCCGCCCAGCTCTCCGCCCGCTATATC
TCCGGCCGTCAGTTGCCGGACAAGGCGATCGACGTGCTCGACACCGCCTGTGCCCGGGTGGCCATCAATCTCACCACGCC
GCCGCGCGCGGTCAGCCACCTGCAAAACGCGCTGCGCCAGCGCGAGCTGGAGATCCGCCAGCTGGAGCGCCAGAGCCTGA
TCGGCCTCGGTGACAACGCAGAGCGCCTGGCAGAGTTGCAGACGGCCCAGCAGGCTTGCCGCGAAGAGCTGGCCGAGCAG
GAGGCCCGCTGGCAGCAGCAGCAGGGGCTGGTACACCAGATTGTCGAGCTGCGCGCCGCCCTGCTGGCCGATCAGCAGGA
TGAGATGCTGGCCAGAGAGGCGCTGGATCTCGCCGATGCGCCCCTCGATCCGCAAGCCGCTGCCGAGCAGCTGGCCACCC
TCGAGCGCGAGCTGGCCGAGCTGCAGCAAGGAGAAGTGCTGGTCTCCGCCCACGTCGACAAGACCCAGATTGCTGCCGTC
ATCGCCGAGTGGACCGGGGTGCCCCTCAATCGCATCTCGCAAGGGGAGCTGGACGTGGTGACCCGATTGCCGGAGTACCT
CGGCGAGCTTATCAAGGGGCAGGATGTGGCGGTGGCCCACCTGCACAAGCACCTGCTGACCGCCCGCGCCGATTTGCGTC
GTCCCGGCCGCCCGCTCGGCGCCTTCCTGCTGGTAGGGCCGAGCGGCGTCGGCAAGACCGAAACCGTGCTGCAGATTGCC
GAGCTGATGTTCGGTGGTCGCCAGTATCTGACCACCATCAACATGTCCGAGTATCAAGAGAAGCACACCGTTTCCCGTCT
CATCGGCTCGCCGCCCGGCTACGTCGGTTTCGGCGAGGGGGGCGTGCTGACCGAGGCCATTCGGCAGAAACCTTATTCCG
TGGTGCTGCTGGACGAGGTGGAGAAGGCCCACCCGGACGTGCTCAACCTCTTCTATCAGGCGTTCGACAAGGGCGAGCTG
GCGGATGGGGAAGGGCGCATCATCGACTGCAAGAACGTGGTTTTCTTCCTCACCTCCAACCTCGGTTTCCAGACCATAGT
CGATCACGCCGAGCAGCCAGACGTGCTGCTGGATGCTCTCTATCCGGAGCTCGCCGCCTTCTTCAAGCCGGCGCTGCTGG
CACGGATGGAGGTGATCCCCTACCTGCCGCTCGGTCACGACACCCTGGTGCAGATCGTCGGCGGCAAGCTCAACCGGCTG
GTCAAGCTGCTCAAGGAGCGTTTCGGTGCCGAGGTGGTGCTGGACGACGAGGTGGCCGAGGAGATCCTGCTGCGGGCCAA
TCGCAGCGAGAACGGCGCCCGCATGCTGGAATCCGTCATCGATGGGGCCCTGCTGCCGCCGGTCTCCCTGCAACTGCTGC
AACGCCTCTCGGCCGGTGAACCCATCAAGCGCGTCCACTTTTCCGTGGCCGATCACCAGTTCGTGGCCGAGGTGGGGGCT
TGA