Detailed information of component protein


Summary

Component ID T6CP010919
Component protein type TssL
T6SS ID (Type) T6SS00788 experimental (Type i1)
Strain Aeromonas dhakensis strain SSU
Replicon chromosome
Sequence Protein sequence (260 a.a.); Nucleotide sequence (780 bp)
Reference
[1] Suarez G, Sierra JC, Sha J, et al. Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog. 2008 Apr;44(4):344-61. Epub 2007 Oct 24. PMID: 18037263
[2] Suarez G, Sierra JC, Erova TE, et al. A type VI secretion system effector protein, VgrG1, from Aeromonas hydrophila that induces host cell toxicity by ADP ribosylation of actin. J Bacteriol. 2010 Jan;192(1):155-68. doi: 10.1128/JB.01260-09. PMID: 19880608
[3] Sha J, Rosenzweig JA, Kozlova EV, et al. Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis. Microbiology (Reading). 2013 Jun;159(Pt 6):1120-1135. doi: 10.1099/mic.0.063495-0. Epub 2013 Mar 21. PMID: 23519162
[4] Suarez G, Sierra JC, Kirtley ML, et al. Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. Microbiology (Reading). 2010 Dec;156(Pt 12):3678-3688. doi: 10.1099/mic.0.041277-0. Epub 2010 Aug 26. PMID: 20798163
[5] Grim CJ, Kozlova EV, Ponnusamy D, et al. Functional genomic characterization of virulence factors from necrotizing fasciitis-causing strains of Aeromonas hydrophila. Appl Environ Microbiol. 2014 Jul;80(14):4162-83. doi: 10.1128/AEM.00486-14. Epub 2014 May 2. PMID: 24795370
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) HMPREF1171_RS04750
Coordinate (Strand) 1037921..1038700 (+)
NCBI ID WP_005299995.1
RefSeq NZ_JH815591
Uniprot ID K1JMB7_9GAMM
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 9.3e-62 39..241
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-218 MTTDIIKNEQLSDLLFDHAEQLDMDSDYWFRLRGQSINPMIDAVTPLLGLVQRVRLLSRYDQVPELYQRVVTEIQAIEQELMAQGYENGVVLSFRYILCTFIDEAVMGRDWGSQSEWSQHSLLARFHNETWGGEKVFVLLARLQEDPVRYRDILEFIYLCLCLGFEGRYKVMSQGREEFERIVRQLHKQLAHEAGGEAPSVFHLDLGQQASRYQLRKQ
Transmembrane helix 219-241 VSLRSLFMGGALILALIFGLYHH
Outside 242-259 QLNNQTQDVLRQLGELLK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9966 - -


  Protein sequence: 260 a.a.    

>T6CP010919 NZ_JH815591:1037921-1038700 [Aeromonas dhakensis] [TssL]
MTTDIIKNEQLSDLLFDHAEQLDMDSDYWFRLRGQSINPMIDAVTPLLGLVQRVRLLSRYDQVPELYQRVVTEIQAIEQE
LMAQGYENGVVLSFRYILCTFIDEAVMGRDWGSQSEWSQHSLLARFHNETWGGEKVFVLLARLQEDPVRYRDILEFIYLC
LCLGFEGRYKVMSQGREEFERIVRQLHKQLAHEAGGEAPSVFHLDLGQQASRYQLRKQVSLRSLFMGGALILALIFGLYH
HQLNNQTQDVLRQLGELLK*

  Nucleotide sequence: 780 bp    

>T6CP010919 NZ_JH815591:1037921-1038700 [Aeromonas dhakensis] [TssL]
ATGACCACGGACATTATCAAGAACGAGCAACTCAGCGACCTGCTGTTCGATCACGCCGAGCAGCTGGACATGGATTCGGA
CTACTGGTTCCGCCTGCGCGGCCAGAGCATCAACCCGATGATCGACGCGGTGACCCCCTTGCTGGGGCTGGTGCAGCGGG
TGCGCCTGCTGTCGCGCTATGACCAGGTGCCCGAGCTCTACCAGCGGGTGGTGACCGAGATCCAGGCCATCGAACAGGAG
CTGATGGCCCAGGGTTACGAGAACGGGGTGGTGCTGTCGTTTCGCTACATCCTCTGCACCTTCATCGACGAGGCCGTGAT
GGGGCGCGACTGGGGCAGCCAGAGCGAGTGGTCCCAGCACTCCCTGCTGGCCCGCTTCCACAACGAGACCTGGGGCGGCG
AGAAGGTGTTCGTGCTGCTGGCGCGCCTGCAGGAAGACCCGGTGCGCTACCGCGACATTCTGGAGTTCATCTACCTGTGC
CTCTGCCTCGGTTTCGAGGGGCGCTACAAGGTGATGAGCCAGGGGCGGGAAGAGTTCGAGCGCATCGTGCGCCAGCTGCA
CAAGCAGTTGGCGCACGAGGCAGGTGGTGAGGCTCCCAGCGTGTTTCACCTCGATCTGGGGCAGCAGGCCTCCCGCTATC
AGCTGCGCAAGCAGGTTTCCCTGCGCAGTCTGTTCATGGGCGGGGCGCTGATCCTGGCGCTGATCTTCGGCCTCTACCAC
CACCAACTCAATAACCAGACCCAGGACGTACTGCGTCAACTGGGCGAATTATTAAAATAA