Detailed information of regulator


Summary

Regulator ID REG0254 experimental
Regulator type protein
Regulatory pathway (from literatures) Transcription regulator
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00788 experimental (Type i1)
Strain Aeromonas dhakensis strain SSU
Replicon chromosome
Sequence Protein sequence (513 a.a.); Nucleotide sequence (1539 bp)
Description Deletion of the vasH gene abrogated the expression and secretion of Hcp and complementation of the mutant in vivo indicating no potential polar effects of the mutation
Reference
External database links

Locus tag (Gene) HMPREF1171_RS04760
Coordinates (Strand) 1041366..1042904 (+)
NCBI ID WP_005299999.1
RefSeq NZ_JH815591
Uniprot ID K1KBF0_9GAMM
KEGG ID -
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
hcp expression ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Sigma54_activat PF00158.28 8.8e-66 198..361
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-512 MEQALAFALALTAQRDEPHLCHWWSSTLHASFQPKGLLLGMLDVSGRQLECKGWVRGKDVALGLAVDDFSHPLAYVLHKAQSRTWDSLYGGARIEHAGFRALLADLGQQCGLHAYPLLDSNGKPFAVLALMDEGEVLRAWADGPELAQLSQVFCNQLTLIRDLGRSRRDQGVLRDSLRQMKGEGERLRQHEKLIGDQLVGQSAVIRGLREQINQAGQHKLTVLIQGETGSGKEVVARLVHQCSDRANKPFVAINCAAIPENLIESELFGYQKGAFSGALANKTGLVAQANGGTLFLDEVGDMPAAMQAKLLRVLETRSYRPLGAEQEYHSDFRLIAATHQPLTRHVEEGQFRADLYHRLCQCLLLIAPLREHIEDVPLLCQHFMAQFAAQDGKTLAGLQRKFLKQLQTYDFPGNVRELRNLLEVACAHTRSGEEVGLEALPPELRERVCVELPGYMDDYNHIRDLRRAMQQYEASVIEARLRHFHGNRMLVAESLNIPKRTLDHKCQKLEVN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9214 - -


  Protein sequence of regulator: 513 a.a.    .

>REG0254 NZ_JH815591:1041366-1042904 [Aeromonas dhakensis]
MEQALAFALALTAQRDEPHLCHWWSSTLHASFQPKGLLLGMLDVSGRQLECKGWVRGKDVALGLAVDDFSHPLAYVLHKA
QSRTWDSLYGGARIEHAGFRALLADLGQQCGLHAYPLLDSNGKPFAVLALMDEGEVLRAWADGPELAQLSQVFCNQLTLI
RDLGRSRRDQGVLRDSLRQMKGEGERLRQHEKLIGDQLVGQSAVIRGLREQINQAGQHKLTVLIQGETGSGKEVVARLVH
QCSDRANKPFVAINCAAIPENLIESELFGYQKGAFSGALANKTGLVAQANGGTLFLDEVGDMPAAMQAKLLRVLETRSYR
PLGAEQEYHSDFRLIAATHQPLTRHVEEGQFRADLYHRLCQCLLLIAPLREHIEDVPLLCQHFMAQFAAQDGKTLAGLQR
KFLKQLQTYDFPGNVRELRNLLEVACAHTRSGEEVGLEALPPELRERVCVELPGYMDDYNHIRDLRRAMQQYEASVIEAR
LRHFHGNRMLVAESLNIPKRTLDHKCQKLEVN*

  Nucleotide sequence of regulator: 1539 bp    .

>REG0254 NZ_JH815591:1041366-1042904 [Aeromonas dhakensis]
ATGGAGCAAGCCCTCGCATTTGCCCTGGCCCTCACCGCCCAGCGCGATGAGCCGCATCTGTGCCACTGGTGGAGCTCGAC
CCTTCATGCCAGCTTCCAGCCCAAGGGGCTGCTGCTCGGCATGCTGGATGTGAGCGGCCGCCAGCTCGAATGCAAAGGCT
GGGTCAGGGGGAAGGACGTGGCGCTGGGGCTGGCGGTGGACGATTTTTCCCACCCTCTTGCCTATGTGTTGCACAAGGCT
CAGTCCCGCACCTGGGACTCCCTCTACGGCGGTGCCCGCATCGAGCACGCCGGTTTTCGCGCACTGCTGGCCGACCTTGG
CCAGCAGTGCGGCCTGCACGCCTATCCGCTGCTGGATAGCAACGGCAAGCCGTTTGCCGTGCTGGCCCTGATGGATGAGG
GGGAGGTGCTGCGGGCCTGGGCCGACGGCCCCGAGCTGGCCCAGCTCTCCCAGGTCTTTTGCAACCAGCTCACCCTCATT
CGCGATCTCGGTCGCAGCCGGCGGGATCAGGGGGTACTGCGTGACTCCCTGCGCCAGATGAAGGGAGAGGGGGAGCGACT
GCGTCAGCACGAGAAGCTGATCGGCGATCAGCTGGTCGGCCAGTCGGCGGTGATCCGCGGGCTGCGCGAGCAGATCAACC
AGGCTGGCCAGCACAAGCTGACCGTGCTGATCCAGGGGGAGACCGGCAGCGGCAAGGAGGTGGTGGCACGGCTGGTGCAT
CAGTGCTCCGATCGCGCCAACAAGCCGTTTGTCGCCATCAACTGCGCCGCCATCCCGGAGAACCTCATCGAGAGCGAGCT
GTTCGGCTACCAGAAGGGGGCCTTCTCTGGCGCCCTTGCTAACAAGACCGGGCTGGTGGCCCAGGCCAACGGCGGTACCC
TGTTTCTGGACGAGGTGGGCGACATGCCCGCCGCCATGCAGGCCAAGCTGCTGCGGGTGCTGGAAACCCGCAGTTACCGG
CCGCTCGGGGCCGAGCAGGAGTATCACTCCGATTTTCGCCTCATCGCCGCCACCCATCAGCCGCTCACCCGTCATGTGGA
GGAGGGGCAGTTTCGGGCCGACCTCTACCACCGTCTCTGCCAGTGCCTGCTGCTGATCGCCCCCCTGCGCGAGCATATCG
AGGATGTGCCGCTCTTGTGCCAGCACTTCATGGCCCAGTTTGCCGCTCAGGATGGCAAGACGCTCGCCGGCTTGCAGCGC
AAGTTCTTGAAACAGCTGCAGACCTATGACTTCCCCGGCAACGTGCGCGAGCTGCGCAACCTGCTGGAGGTGGCCTGTGC
CCATACCCGCTCCGGGGAGGAGGTGGGGCTGGAGGCGTTGCCGCCAGAGCTGCGCGAGCGGGTCTGCGTCGAGTTGCCCG
GCTACATGGATGACTACAACCACATCCGCGATCTGCGCCGGGCCATGCAGCAGTACGAGGCCTCCGTCATCGAGGCGCGC
CTGCGCCACTTCCACGGCAACCGGATGCTGGTGGCCGAGAGTCTCAACATTCCCAAGCGCACCCTGGATCACAAGTGCCA
GAAACTGGAGGTGAACTGA