SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00361
Cognate effector	EFF76650
Related T6SS (Type)	T6SS00788 (Type i1)
Strain	Aeromonas dhakensis strain SSU
Replicon	chromosome
Sequence	Protein sequence (269 a.a.); Nucleotide sequence (807 bp)
Description	The tseC tsiC mutant was efficiently killed by 10⁴-fold when exposed to WT SSU, and complementation with a plasmid-borne tsiC fully protected the tseC tsiC mutant from killing, indicating that TsiC is the cognate immunity protein to TseC.
Reference	[1] Liang X, Moore R, Wilton M, et al. Identification of divergent type VI secretion effectors using a conserved chaperone domain. Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):9106-11. doi: 10.1073/pnas.1505317112. Epub 2015 Jul 6. PMID: 26150500

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	HMPREF1171_RS12050 (tsiC)
Coordinates (Strand)	2752679..2753485 (-)
NCBI ID	WP_005303621.1
RefSeq	NZ_JH815591
Uniprot ID	K1J935_9GAMM
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-54	MNNTNEVAEANEQETPFHLLPQEEQQFIIDTNQKRVREAQTLHEWDIRVSTAFA
Transmembrane helix	55-77	WPMILIVVGSMMLFGGAAVWFAG
Outside	78-80	DND
Transmembrane helix	81-103	WTFVYMMIGMSIVMTLYLRYLVM
Inside	104-137	ADKHYHYRLTTEGLIVTYHDAIPEVAYTIVRGLA
Transmembrane helix	138-160	WLGVVVCVMAMAVLGPLALVGAG
Outside	161-268	GMAIFAVFFTNFEKKVSVEYTVFNKDKAYVVKVVKNRSLIRFDIIPFSWFEFSYLYCNEGELYKILSSLMKELGCDEYREFKSELSLRRAPVGVLDVGTPRRLDEINK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9929	-	-

Protein sequence: 269 a.a. .

                                    >IMU00361 NZ_JH815591:c2753485-2752679 [Aeromonas dhakensis]
MNNTNEVAEANEQETPFHLLPQEEQQFIIDTNQKRVREAQTLHEWDIRVSTAFAWPMILIVVGSMMLFGGAAVWFAGDND
WTFVYMMIGMSIVMTLYLRYLVMADKHYHYRLTTEGLIVTYHDAIPEVAYTIVRGLAWLGVVVCVMAMAVLGPLALVGAG
GMAIFAVFFTNFEKKVSVEYTVFNKDKAYVVKVVKNRSLIRFDIIPFSWFEFSYLYCNEGELYKILSSLMKELGCDEYRE
FKSELSLRRAPVGVLDVGTPRRLDEINK*

                                

Nucleotide sequence: 807 bp .

                                    >IMU00361 NZ_JH815591:c2753485-2752679 [Aeromonas dhakensis]
ATGAACAACACGAATGAAGTAGCTGAGGCAAACGAGCAAGAAACCCCTTTTCATCTGCTGCCGCAGGAAGAGCAACAATT
CATAATCGATACCAATCAGAAAAGGGTGCGTGAGGCACAAACTCTTCATGAATGGGATATCCGGGTATCTACAGCATTTG
CATGGCCAATGATATTAATAGTCGTTGGCTCAATGATGCTATTTGGAGGTGCGGCGGTATGGTTTGCTGGAGATAATGAT
TGGACTTTTGTTTATATGATGATCGGTATGTCGATAGTGATGACGCTCTATTTACGTTACTTGGTGATGGCGGATAAACA
CTACCATTACCGGCTGACGACAGAAGGCTTAATCGTCACTTATCATGATGCCATTCCTGAAGTGGCCTACACCATAGTGC
GTGGCTTGGCTTGGCTTGGGGTGGTGGTTTGCGTGATGGCTATGGCTGTATTGGGGCCGCTAGCGCTGGTTGGTGCAGGT
GGTATGGCAATTTTTGCTGTTTTTTTTACTAACTTTGAAAAGAAAGTATCAGTTGAATACACGGTTTTCAATAAAGATAA
AGCATATGTTGTGAAAGTGGTTAAAAATAGAAGCCTTATTCGCTTTGATATCATTCCATTCAGTTGGTTCGAGTTCTCTT
ATTTATATTGCAATGAAGGTGAACTATATAAAATATTGTCAAGTTTAATGAAAGAGCTTGGGTGTGATGAATATAGAGAG
TTTAAAAGTGAATTATCTCTTCGCAGAGCTCCCGTTGGTGTATTGGATGTTGGCACGCCAAGAAGGCTGGATGAGATCAA
TAAGTAA

                                