Detailed information of component protein


Summary

Component ID T6CP259518
Component protein type TssH
T6SS ID (Type) T6SS00788 experimental (Type i1)
Strain Aeromonas dhakensis strain SSU
Replicon chromosome
Sequence Protein sequence (750 a.a.); Nucleotide sequence (2250 bp)
Reference
[1] Suarez G, Sierra JC, Sha J, et al. Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog. 2008 Apr;44(4):344-61. Epub 2007 Oct 24. PMID: 18037263
[2] Suarez G, Sierra JC, Erova TE, et al. A type VI secretion system effector protein, VgrG1, from Aeromonas hydrophila that induces host cell toxicity by ADP ribosylation of actin. J Bacteriol. 2010 Jan;192(1):155-68. doi: 10.1128/JB.01260-09. PMID: 19880608
[3] Sha J, Rosenzweig JA, Kozlova EV, et al. Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis. Microbiology (Reading). 2013 Jun;159(Pt 6):1120-1135. doi: 10.1099/mic.0.063495-0. Epub 2013 Mar 21. PMID: 23519162
[4] Suarez G, Sierra JC, Kirtley ML, et al. Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. Microbiology (Reading). 2010 Dec;156(Pt 12):3678-3688. doi: 10.1099/mic.0.041277-0. Epub 2010 Aug 26. PMID: 20798163
[5] Grim CJ, Kozlova EV, Ponnusamy D, et al. Functional genomic characterization of virulence factors from necrotizing fasciitis-causing strains of Aeromonas hydrophila. Appl Environ Microbiol. 2014 Jul;80(14):4162-83. doi: 10.1128/AEM.00486-14. Epub 2014 May 2. PMID: 24795370
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) HMPREF1171_RS04840
Coordinate (Strand) 1058805..1061054 (+)
NCBI ID WP_005300030.1
RefSeq NZ_JH815591
Uniprot ID K1JBQ2_9GAMM
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 7.8e-49 482..643
AAA_lid_9 PF17871.3 3.4e-29 346..449
ClpB_D2-small PF10431.11 4.7e-23 649..729
Clp_N PF02861.22 9.1e-13 13..62
AAA PF00004.31 2.4e-11 207..297
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-749 MLNKDLEKTLNEAFKLARDERHEFMTVEHLLLALLDNSSANEALSSCGADVEQMRKEISAFISQTTPLIPRDDEDRETQPTLGFQRVLQRAVFHVQSSGNTEVSGANVLVAIFSEQESQAAYFLKKAEISRLDVVNFLSHGVRKDSKPDNSNNVESDDEVSAAQIESFATNLNQLVLEGRIDPLIGRDQELNRTIQVLCRRRKNNPLLVGEAGVGKTAIAEGLAYRIVKGDVPEVIAGSTIYSLDMGSLLAGTKYRGDFEKRLKVLLKQIERQEGAILFIDEIHTIIGAGAASGGQLDAANLIKPLLSNGLLRCVGSTTYQEYSQIFEKDRALARRFQKIDIVEPSVDDTTRILMGLKSRYEAHHGVRYTVKAIRAAVELSAKYINDRHLPDKAIDVIDEAGAGQRLLPVSKRKKVINVQEIETIVAKIARIPEKSVSSSDKEVLKNLERNLKMVVFGQDKAIEVLTDSIRLSRSGLGNERRPVGCFLFAGPTGVGKTEVTQQLGKALGVEVLRFDMSEYMERHTVSRLIGAPPGYVGFEQGGLLTDSVLKHPHSVVLLDEIEKAHPDVFNLLLQVMDNGTLTDNNGRKADFRNVILVMTTNAGVQETQRKSIGFQQQDMSHDAMAVINKTFSPEFRNRLDHIIWFNHLDMQIIHQVVDKFIVELQVQLDAKGVSLEVSEGVRHWLAEKGYDRAMGARPMGRVIQEHLKKPLANEILFGALVDGGVVKVELINDSLSFDYQVNVEAVSH


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9356 - -


  Protein sequence: 750 a.a.    

>T6CP259518 NZ_JH815591:1058805-1061054 [Aeromonas dhakensis] [TssH]
MLNKDLEKTLNEAFKLARDERHEFMTVEHLLLALLDNSSANEALSSCGADVEQMRKEISAFISQTTPLIPRDDEDRETQP
TLGFQRVLQRAVFHVQSSGNTEVSGANVLVAIFSEQESQAAYFLKKAEISRLDVVNFLSHGVRKDSKPDNSNNVESDDEV
SAAQIESFATNLNQLVLEGRIDPLIGRDQELNRTIQVLCRRRKNNPLLVGEAGVGKTAIAEGLAYRIVKGDVPEVIAGST
IYSLDMGSLLAGTKYRGDFEKRLKVLLKQIERQEGAILFIDEIHTIIGAGAASGGQLDAANLIKPLLSNGLLRCVGSTTY
QEYSQIFEKDRALARRFQKIDIVEPSVDDTTRILMGLKSRYEAHHGVRYTVKAIRAAVELSAKYINDRHLPDKAIDVIDE
AGAGQRLLPVSKRKKVINVQEIETIVAKIARIPEKSVSSSDKEVLKNLERNLKMVVFGQDKAIEVLTDSIRLSRSGLGNE
RRPVGCFLFAGPTGVGKTEVTQQLGKALGVEVLRFDMSEYMERHTVSRLIGAPPGYVGFEQGGLLTDSVLKHPHSVVLLD
EIEKAHPDVFNLLLQVMDNGTLTDNNGRKADFRNVILVMTTNAGVQETQRKSIGFQQQDMSHDAMAVINKTFSPEFRNRL
DHIIWFNHLDMQIIHQVVDKFIVELQVQLDAKGVSLEVSEGVRHWLAEKGYDRAMGARPMGRVIQEHLKKPLANEILFGA
LVDGGVVKVELINDSLSFDYQVNVEAVSH*

  Nucleotide sequence: 2250 bp    

>T6CP259518 NZ_JH815591:1058805-1061054 [Aeromonas dhakensis] [TssH]
ATGTTGAATAAAGATCTAGAAAAGACGCTGAACGAGGCTTTCAAACTGGCCCGTGACGAGCGCCACGAATTCATGACCGT
CGAGCACCTGTTGTTAGCCCTGCTGGATAACAGCTCAGCCAATGAGGCGCTGAGTTCTTGTGGTGCTGACGTGGAGCAGA
TGCGCAAAGAGATCAGCGCATTCATCAGTCAAACAACCCCGCTTATTCCCCGTGATGATGAAGACCGCGAGACCCAGCCC
ACACTGGGCTTCCAGCGGGTGCTGCAACGCGCCGTGTTCCACGTTCAGTCCTCCGGCAACACCGAGGTCAGTGGAGCCAA
CGTGCTGGTTGCCATCTTCAGCGAGCAGGAGTCCCAGGCTGCTTACTTCCTGAAAAAGGCCGAGATCAGCCGGCTGGATG
TGGTCAACTTCCTCTCCCATGGGGTACGTAAAGACAGCAAACCGGACAACAGCAACAACGTCGAGTCTGACGATGAGGTC
TCTGCTGCCCAGATTGAGAGCTTTGCCACCAACCTCAATCAGCTGGTACTGGAAGGGCGTATCGATCCCCTCATCGGGCG
CGATCAGGAGCTCAACCGTACCATCCAGGTGCTCTGCCGCCGTCGCAAGAACAACCCCTTGCTGGTGGGCGAAGCCGGGG
TGGGCAAGACGGCCATTGCGGAAGGGCTGGCCTATCGTATCGTCAAGGGCGATGTACCCGAGGTGATTGCGGGCAGTACC
ATCTACTCCCTGGATATGGGGTCGCTGCTGGCAGGGACCAAATATCGCGGTGACTTCGAGAAGCGCCTCAAGGTACTGCT
CAAGCAGATCGAGCGCCAGGAAGGGGCGATCCTCTTCATCGACGAGATCCACACCATCATAGGGGCGGGGGCTGCCTCCG
GTGGTCAGCTGGATGCGGCCAACCTCATCAAGCCGCTGCTCTCCAATGGCTTGCTGCGTTGCGTCGGCTCTACCACCTAC
CAGGAATACTCCCAGATCTTTGAAAAAGACCGCGCCCTGGCGCGTCGCTTCCAGAAGATCGACATCGTCGAGCCCAGCGT
GGATGACACCACGCGCATCCTGATGGGCCTCAAGAGCCGCTATGAGGCTCACCACGGGGTACGTTACACCGTCAAGGCCA
TCCGTGCTGCGGTGGAGCTCTCCGCCAAATACATCAATGACCGCCATCTGCCGGACAAGGCGATCGACGTGATCGACGAG
GCCGGGGCGGGTCAACGCTTGCTGCCGGTGAGCAAGCGCAAGAAAGTGATCAATGTGCAGGAGATCGAAACCATTGTCGC
CAAGATTGCCCGCATCCCGGAGAAATCAGTCTCCTCTTCCGACAAGGAAGTGCTGAAGAATCTGGAGCGCAATCTCAAGA
TGGTGGTCTTTGGCCAGGACAAGGCCATCGAGGTACTGACAGACTCCATTCGCCTCTCCCGCTCAGGGCTGGGCAACGAG
CGTCGCCCTGTGGGCTGCTTCCTGTTTGCCGGCCCGACCGGGGTAGGCAAGACCGAGGTCACCCAGCAGCTTGGCAAGGC
GCTTGGCGTGGAGGTGCTGCGCTTTGATATGTCCGAGTACATGGAGCGCCATACCGTCTCTCGCCTTATCGGGGCGCCTC
CCGGTTATGTGGGGTTCGAGCAGGGAGGCCTGCTGACCGACTCTGTCCTCAAGCATCCGCACTCAGTGGTACTGCTGGAC
GAGATCGAAAAAGCGCATCCGGACGTGTTCAACCTGCTGCTGCAGGTGATGGACAACGGTACCCTGACTGACAACAACGG
GCGCAAGGCGGATTTTCGCAACGTGATCCTAGTGATGACCACCAACGCGGGGGTACAGGAGACCCAGCGCAAGTCCATTG
GCTTCCAGCAGCAGGACATGAGCCACGATGCCATGGCGGTGATCAACAAGACGTTCAGTCCGGAGTTTCGCAACCGGCTT
GATCACATCATCTGGTTCAACCATCTGGACATGCAGATCATCCATCAGGTGGTCGACAAGTTCATCGTCGAGTTGCAGGT
TCAGCTGGATGCGAAAGGGGTATCCCTTGAAGTGTCCGAGGGTGTCCGCCACTGGCTGGCCGAGAAAGGATATGACAGGG
CCATGGGGGCAAGACCCATGGGGCGGGTCATCCAGGAACACCTCAAGAAACCCCTGGCCAACGAGATCCTGTTCGGGGCG
CTGGTCGATGGGGGGGTTGTGAAGGTTGAGTTGATAAACGACAGCCTCAGCTTTGACTATCAGGTCAATGTTGAAGCCGT
TTCCCACTAA