Detailed information of component protein


Summary

Component ID T6CP010921
Component protein type TssA
T6SS ID (Type) T6SS00788 experimental (Type i1)
Strain Aeromonas dhakensis strain SSU
Replicon chromosome
Sequence Protein sequence (482 a.a.); Nucleotide sequence (1446 bp)
Reference
[1] Suarez G, Sierra JC, Sha J, et al. Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog. 2008 Apr;44(4):344-61. Epub 2007 Oct 24. PMID: 18037263
[2] Suarez G, Sierra JC, Erova TE, et al. A type VI secretion system effector protein, VgrG1, from Aeromonas hydrophila that induces host cell toxicity by ADP ribosylation of actin. J Bacteriol. 2010 Jan;192(1):155-68. doi: 10.1128/JB.01260-09. PMID: 19880608
[3] Sha J, Rosenzweig JA, Kozlova EV, et al. Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis. Microbiology (Reading). 2013 Jun;159(Pt 6):1120-1135. doi: 10.1099/mic.0.063495-0. Epub 2013 Mar 21. PMID: 23519162
[4] Suarez G, Sierra JC, Kirtley ML, et al. Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. Microbiology (Reading). 2010 Dec;156(Pt 12):3678-3688. doi: 10.1099/mic.0.041277-0. Epub 2010 Aug 26. PMID: 20798163
[5] Grim CJ, Kozlova EV, Ponnusamy D, et al. Functional genomic characterization of virulence factors from necrotizing fasciitis-causing strains of Aeromonas hydrophila. Appl Environ Microbiol. 2014 Jul;80(14):4162-83. doi: 10.1128/AEM.00486-14. Epub 2014 May 2. PMID: 24795370
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) HMPREF1171_RS04770
Coordinate (Strand) 1043518..1044963 (+)
NCBI ID WP_005300003.1
RefSeq NZ_JH815591
Uniprot ID K1JFN2_9GAMM
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VasJ PF16989.7 2.5e-79 232..474
ImpA_N PF06812.14 2.8e-15 13..110
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-481 MSYQHPWCARLLTSLPDEQIRGAVLADEPRWDYVETELVKLGSLAHSQVDLNAVAEACLGLLESRTKDMRVLAQLLRCLQHPAKATPLGAAISLLEAWIQAYWLLAWPGNASQKQRLMVQIVKRFEGALPRICESASAAELAQLLAQAEQLEQVWLAQCPDKGELLDPLVMGLKRAQRQQVAQAQADAAGQPQSSSAAATGSPASVASTASGAGAMVLSGGGGSAGIDVDSSNDRAWRQTQLKVAELLIERQPEAAVGYRLRRHAVWAGITAVPMSGAGNKTPLAPMSADMVDEYRAAMNAPDQGLWQRIEQSLTLAPYWFEGHRLSAEVAQKLGFGAVAQAIAEELGTFLQRLPALRELAFSDGSPFLSPECSRWLQPAKGGSAGSGETGLAEEVAQRHGEQGVAAALALLDERIAQLKEPRDRFHALLVQAELLAQEGMEALARQHYQHLWQEASRLGLSHWEPGLVNRLESLAAPLSK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9899 - -


  Protein sequence: 482 a.a.    

>T6CP010921 NZ_JH815591:1043518-1044963 [Aeromonas dhakensis] [TssA]
MSYQHPWCARLLTSLPDEQIRGAVLADEPRWDYVETELVKLGSLAHSQVDLNAVAEACLGLLESRTKDMRVLAQLLRCLQ
HPAKATPLGAAISLLEAWIQAYWLLAWPGNASQKQRLMVQIVKRFEGALPRICESASAAELAQLLAQAEQLEQVWLAQCP
DKGELLDPLVMGLKRAQRQQVAQAQADAAGQPQSSSAAATGSPASVASTASGAGAMVLSGGGGSAGIDVDSSNDRAWRQT
QLKVAELLIERQPEAAVGYRLRRHAVWAGITAVPMSGAGNKTPLAPMSADMVDEYRAAMNAPDQGLWQRIEQSLTLAPYW
FEGHRLSAEVAQKLGFGAVAQAIAEELGTFLQRLPALRELAFSDGSPFLSPECSRWLQPAKGGSAGSGETGLAEEVAQRH
GEQGVAAALALLDERIAQLKEPRDRFHALLVQAELLAQEGMEALARQHYQHLWQEASRLGLSHWEPGLVNRLESLAAPLS
K*

  Nucleotide sequence: 1446 bp    

>T6CP010921 NZ_JH815591:1043518-1044963 [Aeromonas dhakensis] [TssA]
ATGAGCTATCAACACCCCTGGTGTGCACGCCTGCTCACCAGCCTGCCGGATGAACAGATCAGAGGCGCGGTGCTGGCCGA
TGAGCCGCGCTGGGACTATGTGGAGACCGAGCTGGTCAAGCTGGGCTCCCTCGCTCACAGCCAGGTCGATCTCAATGCGG
TGGCCGAGGCCTGCCTCGGGTTGCTGGAGAGCCGTACCAAGGACATGCGGGTGCTGGCCCAGCTGCTGCGCTGCCTGCAG
CACCCAGCCAAGGCAACCCCGCTGGGGGCGGCGATCAGCCTGCTGGAGGCCTGGATCCAGGCCTACTGGCTGTTGGCCTG
GCCCGGCAATGCCAGCCAGAAACAGCGGCTGATGGTGCAAATCGTCAAGCGCTTCGAAGGGGCACTGCCGCGTATTTGCG
AGAGCGCTTCGGCAGCCGAGCTGGCCCAGTTGCTGGCCCAGGCCGAGCAGCTGGAGCAGGTCTGGCTGGCGCAGTGTCCC
GACAAGGGAGAGCTGCTCGACCCGTTGGTGATGGGGCTGAAACGGGCCCAGCGCCAGCAGGTGGCTCAGGCGCAGGCTGA
TGCTGCTGGGCAGCCCCAGAGCAGCAGCGCGGCGGCAACGGGCTCCCCGGCCTCGGTGGCATCGACCGCCAGCGGTGCCG
GCGCCATGGTACTCAGCGGTGGTGGCGGCAGTGCCGGCATCGATGTCGACAGCTCCAACGATCGCGCCTGGCGCCAGACT
CAGCTCAAGGTGGCGGAGCTCCTGATCGAGCGCCAGCCGGAGGCGGCAGTGGGCTATCGGCTGCGCCGTCATGCCGTCTG
GGCCGGGATCACGGCGGTTCCCATGAGCGGGGCGGGCAATAAAACCCCGCTGGCGCCCATGTCGGCGGACATGGTGGATG
AGTACCGCGCCGCCATGAACGCCCCTGATCAGGGGCTCTGGCAGCGCATCGAGCAGAGCCTGACCCTGGCGCCTTACTGG
TTCGAGGGGCACAGGCTCTCGGCCGAGGTGGCCCAGAAACTCGGCTTTGGAGCGGTGGCCCAGGCGATCGCCGAGGAGCT
CGGGACTTTTCTGCAGCGCCTGCCGGCCCTGCGGGAGCTCGCCTTCAGCGACGGCTCGCCGTTTCTCTCCCCCGAGTGCA
GCCGCTGGCTGCAACCGGCCAAGGGCGGCAGTGCCGGCAGTGGCGAAACAGGGCTGGCCGAGGAGGTCGCCCAGCGCCAT
GGCGAGCAGGGGGTCGCGGCGGCGCTCGCCCTGCTCGATGAGCGGATTGCGCAGTTGAAAGAGCCAAGAGATCGTTTCCA
CGCCTTGCTGGTGCAGGCGGAGCTGTTGGCGCAGGAAGGCATGGAGGCGCTTGCGCGCCAGCACTATCAACACTTGTGGC
AAGAGGCCAGTCGCCTCGGGTTGTCGCACTGGGAGCCCGGGCTGGTCAACCGCCTGGAGAGCCTGGCGGCGCCGTTGTCG
AAATGA