Detailed information of regulator
Summary
| Regulator ID | REG0283  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | QS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00939 (Type i5) |
| Strain | Vibrio alginolyticus 12G01 |
| Replicon | chromosome |
| Sequence | Protein sequence (293 a.a.); Nucleotide sequence (879 bp) |
| Description | LysR family protein,identified to bind to the promoters of both aphA and luxR. |
| Reference |
External database links
| Locus tag (Gene) | V12G01_06711 (HP) |
| Coordinates (Strand) | 47707..48585 (-) |
| NCBI ID | 91188835 |
| GenBank | AAPS01000024 |
| Uniprot ID | UPI0000D542C4 |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| V12G01-21538 (luxR) promoter | TGAGTGTAA TGGTGCATATGCACAAGGTCAA | |
| V12G01-14830 (aphA) promoter | CACAAGTTAACACTATTGATTATTATGTTAGTTTT |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| HTH_1 | PF00126.29 | 1.1e-16 | 3..62 |
| LysR_substrate | PF03466.22 | 1.6e-15 | 108..287 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-292 | MNLTQVEAFCTIAECGSVSEAARQLDCNRTKLSMSIKALEKDLDVELFNRTGNQLTLSEAGKAIYKDCEHLLIIAQRVRKTCAQISEGFNAEVWVARDDSLPDDLWQELSHTLNNRFPSTTFNLILASSGDLANLVSTHQVDFAFGVDYERVDDPRIVYNPLGKIRMMSVCSVEHRLSKMRRVSDEDLRSQMQALMVYLNEKDNPELQPFSTRYIGFSSFDYMLNTILEEDAWGVLPEPLIRHYLRQQKLAVIKHTYGLTQEDYCMFSPNGHTEHPAMTWLADKLSEYLFDF |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9456 | - | - |
Protein sequence of regulator: 293 a.a. .
>REG0283 AAPS01000024:c48585-47707 [Vibrio alginolyticus 12G01]
MNLTQVEAFCTIAECGSVSEAARQLDCNRTKLSMSIKALEKDLDVELFNRTGNQLTLSEAGKAIYKDCEHLLIIAQRVRK
TCAQISEGFNAEVWVARDDSLPDDLWQELSHTLNNRFPSTTFNLILASSGDLANLVSTHQVDFAFGVDYERVDDPRIVYN
PLGKIRMMSVCSVEHRLSKMRRVSDEDLRSQMQALMVYLNEKDNPELQPFSTRYIGFSSFDYMLNTILEEDAWGVLPEPL
IRHYLRQQKLAVIKHTYGLTQEDYCMFSPNGHTEHPAMTWLADKLSEYLFDF*
MNLTQVEAFCTIAECGSVSEAARQLDCNRTKLSMSIKALEKDLDVELFNRTGNQLTLSEAGKAIYKDCEHLLIIAQRVRK
TCAQISEGFNAEVWVARDDSLPDDLWQELSHTLNNRFPSTTFNLILASSGDLANLVSTHQVDFAFGVDYERVDDPRIVYN
PLGKIRMMSVCSVEHRLSKMRRVSDEDLRSQMQALMVYLNEKDNPELQPFSTRYIGFSSFDYMLNTILEEDAWGVLPEPL
IRHYLRQQKLAVIKHTYGLTQEDYCMFSPNGHTEHPAMTWLADKLSEYLFDF*
Nucleotide sequence of regulator: 879 bp .
>REG0283 AAPS01000024:c48585-47707 [Vibrio alginolyticus 12G01]
ATGAACTTAACTCAAGTAGAAGCCTTCTGCACCATCGCTGAGTGTGGTTCTGTTTCTGAAGCGGCAAGGCAACTCGACTG
CAATCGCACCAAACTCAGTATGTCGATTAAGGCATTAGAGAAAGATCTCGACGTTGAATTGTTTAACCGAACTGGCAACC
AGCTCACCCTATCTGAAGCTGGGAAAGCCATTTACAAAGATTGCGAACACCTACTTATCATCGCACAACGGGTCAGAAAG
ACCTGCGCTCAAATCTCGGAAGGGTTTAACGCGGAAGTTTGGGTAGCACGTGACGACTCCTTACCTGATGATCTCTGGCA
AGAATTATCTCATACATTGAATAACCGCTTTCCTTCAACAACCTTCAACTTAATCTTGGCATCCAGTGGCGACTTAGCCA
ACTTGGTCAGTACCCACCAAGTAGATTTTGCTTTCGGCGTTGATTATGAGCGAGTAGACGATCCTCGTATCGTATATAAC
CCATTAGGCAAGATACGTATGATGTCAGTATGCAGTGTAGAACACCGCCTAAGTAAGATGCGCCGTGTCTCAGACGAAGA
TCTACGTAGTCAGATGCAAGCACTAATGGTGTACCTAAATGAGAAAGACAACCCTGAACTGCAACCTTTCTCGACTCGAT
ATATTGGTTTTTCGAGTTTTGACTACATGCTGAACACCATCTTAGAAGAAGACGCTTGGGGCGTGCTTCCAGAACCATTA
ATCCGCCATTACCTTCGTCAGCAGAAGCTCGCCGTTATCAAACACACATACGGATTGACGCAAGAAGACTACTGTATGTT
TTCACCTAACGGCCATACTGAGCATCCAGCAATGACGTGGTTAGCAGATAAATTGAGCGAGTATTTATTTGACTTCTAA
ATGAACTTAACTCAAGTAGAAGCCTTCTGCACCATCGCTGAGTGTGGTTCTGTTTCTGAAGCGGCAAGGCAACTCGACTG
CAATCGCACCAAACTCAGTATGTCGATTAAGGCATTAGAGAAAGATCTCGACGTTGAATTGTTTAACCGAACTGGCAACC
AGCTCACCCTATCTGAAGCTGGGAAAGCCATTTACAAAGATTGCGAACACCTACTTATCATCGCACAACGGGTCAGAAAG
ACCTGCGCTCAAATCTCGGAAGGGTTTAACGCGGAAGTTTGGGTAGCACGTGACGACTCCTTACCTGATGATCTCTGGCA
AGAATTATCTCATACATTGAATAACCGCTTTCCTTCAACAACCTTCAACTTAATCTTGGCATCCAGTGGCGACTTAGCCA
ACTTGGTCAGTACCCACCAAGTAGATTTTGCTTTCGGCGTTGATTATGAGCGAGTAGACGATCCTCGTATCGTATATAAC
CCATTAGGCAAGATACGTATGATGTCAGTATGCAGTGTAGAACACCGCCTAAGTAAGATGCGCCGTGTCTCAGACGAAGA
TCTACGTAGTCAGATGCAAGCACTAATGGTGTACCTAAATGAGAAAGACAACCCTGAACTGCAACCTTTCTCGACTCGAT
ATATTGGTTTTTCGAGTTTTGACTACATGCTGAACACCATCTTAGAAGAAGACGCTTGGGGCGTGCTTCCAGAACCATTA
ATCCGCCATTACCTTCGTCAGCAGAAGCTCGCCGTTATCAAACACACATACGGATTGACGCAAGAAGACTACTGTATGTT
TTCACCTAACGGCCATACTGAGCATCCAGCAATGACGTGGTTAGCAGATAAATTGAGCGAGTATTTATTTGACTTCTAA