Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00939 experimental
T6SS type Type i5
Strain Vibrio alginolyticus 12G01
Replicon (RefSeq) chromosome (NZ_CH902589)
Location 1440993..1467006
Function anti-bacterial
Comment V.alginolyticus can use its T6SS for interbacterial competition by showing that it is able to kill E. coli in a T6SS-dependent manner. VaT6SS1 is more active under low salt conditions (on LB plates), whereas VaT6SS2 is more active under high salt conditions (on MLB plates). Both T6SSs are active at 30°C, but only VaT6SS2 is also active at 37°C.
References
[1] Salomon D et al (2014) Marker for type VI secretion system effectors. Proc Natl Acad Sci U S A. 111(25):9271-6. [PudMed:24927539]
[2] Salomon D et al (2015) Type VI Secretion System Toxins Horizontally Shared between Marine Bacteria. PLoS Pathog. 11(8):e1005128. [PudMed:26305100]
experimental Experimental investigation has been performed.
T6SS components and genome coordinates

ComponentPAARTagETagFTagGTagHTagJTssATssBTssCTssDTssETssFTssGTssHTssITssJTssKTssLTssM
Number1111111121111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
V12G01_RS31125 1436258..1436833 - 576 491518273 TetR/AcrR family transcriptional regulator
V12G01_RS31130 1437118..1437732 + 615 491518274 glutathione S-transferase
V12G01_RS31135 1437953..1438213 + 261 491518277 hypothetical protein
V12G01_RS31140 1438243..1439472 + 1230 491518279 SpoIIE family protein phosphatase
V12G01_RS31145 1439453..1439914 + 462 491518283 ATP-binding protein
V12G01_RS31150 1439985..1440176 - 192 491518285 hypothetical protein
V12G01_RS31155 1440211..1440546 - 336 491518287 Hpt domain-containing protein
T6CP015465 TagE V12G01_RS31160 1440993..1443143 - 2151 491518297 serine/threonine protein kinase
T6CP015466 TagH V12G01_RS31165 (tagH) 1443456..1444649 + 1194 491518300 type VI secretion system-associated FHA domain protein TagH
T6CP015467 TssJ V12G01_RS31170 (tssJ) 1444661..1445116 + 456 491518302 type VI secretion system lipoprotein TssJ
T6CP015468 TssK V12G01_RS31175 (tssK) 1445138..1446463 + 1326 1168680938 type VI secretion system baseplate subunit TssK
T6CP015469 TssL V12G01_RS31180 (tssL) 1446475..1447767 + 1293 491518308 type VI secretion system protein TssL, long form
T6CP015470 TssM V12G01_RS31185 (tssM) 1447784..1451302 + 3519 491518311 type VI secretion system membrane subunit TssM
T6CP015471 TagF V12G01_RS31190 (tagF) 1451284..1451979 + 696 491518313 type VI secretion system-associated protein TagF
T6CP015472 TagG V12G01_RS31195 1451989..1452783 + 795 491518316 serine/threonine-protein phosphatase
T6CP015473 TssA V12G01_RS31200 (tssA) 1452776..1453891 + 1116 491518317 type VI secretion system protein TssA
T6CP015474 TssB V12G01_RS31205 (tssB) 1453907..1454437 + 531 491518318 type VI secretion system contractile sheath small subunit
T6CP015475 TssC V12G01_RS31210 (tssC) 1454437..1455930 + 1494 491518319 type VI secretion system contractile sheath large subunit
T6CP015476 TssC V12G01_RS31215 (tssC) 1455983..1457494 + 1512 491518320 type VI secretion system contractile sheath large subunit
T6CP015477 TagJ V12G01_RS31220 1457505..1458299 + 795 491518321 hypothetical protein
T6CP015478 TssE V12G01_RS31225 (tssE) 1458307..1458771 + 465 491518322 type VI secretion system baseplate subunit TssE
T6CP015479 TssF V12G01_RS31230 (tssF) 1458768..1460591 + 1824 491518323 type VI secretion system baseplate subunit TssF
T6CP015480 TssG V12G01_RS31235 (tssG) 1460588..1461553 + 966 491518332 type VI secretion system baseplate subunit TssG
T6CP015481 TssH V12G01_RS31240 (tssH) 1461564..1464131 + 2568 491518334 type VI secretion system ATPase TssH
T6CP015482 TssD V12G01_RS31245 1464340..1464819 + 480 491518336 type VI secretion system tube protein Hcp
T6CP015483 TssI V12G01_RS31250 (tssI) 1464869..1466704 + 1836 491518337 type VI secretion system tip protein VgrG
T6CP015484 PAAR V12G01_RS31255 1466716..1467006 + 291 491518338 PAAR domain-containing protein
V12G01_RS31260 1467007..1467846 + 840 491518339 hypothetical protein
V12G01_RS31265 1468208..1469341 + 1134 491518340 exonuclease SbcCD subunit D
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0275 experimental V12G01_21538 (LuxR) protein QS regulatory pathway Vibrio alginolyticus 12G01 chromosome (AAPS01000053) 13551..14165 (-) 91187814 T6SS2 transcripts (↓); V12G01-18672 (vqsA) promotor
REG0276 experimental V12G01_19736 (TfoX) protein Transcription regulator Vibrio alginolyticus 12G01 chromosome (AAPS01000019) 47689..48276 (-) 91189146 T6SS2 transcripts (↓)
REG0277 experimental V12G01_20658 (TfoY) protein Transcription regulator Vibrio alginolyticus 12G01 chromosome (AAPS01000039) 21665..22255 (+) 91188194 T6SS transcripts (↓)
REG0278 experimental V12G01_20201 (H-NS) protein Transcription regulator Vibrio alginolyticus 12G01 chromosome (AAPS01000015) 63783..64190 (+) 91189474 VaT6SS1 transcripts (↑)
REG0279 experimental V12G01_18672 (vqsA) protein QS regulatory pathway Vibrio alginolyticus 12G01 chromosome (AAPS01000003) 114208..115086 (+) 91191289 V12G01-21538 (luxR) promoter (↓); V12G01-14830 (aphA) promoter (↓); V12G01-18672 (vqsA) promotor (↓)
REG0280 experimental V12G01_10813 (AsnC) protein QS regulatory pathway Vibrio alginolyticus 12G01 chromosome (AAPS01000004) 181050..181484 (-) 91191052 V12G01-21538 (luxR) promoter; V12G01-14830 (aphA) promoter
REG0281 experimental V12G01_02325 (HP) protein QS regulatory pathway Vibrio alginolyticus 12G01 chromosome (AAPS01000004) 115263..116411 (+) 91190993 V12G01-21538 (luxR) promoter; V12G01-14830 (aphA) promoter
REG0282 experimental V12G01_01665 (HP) protein QS regulatory pathway Vibrio alginolyticus 12G01 chromosome (AAPS01000007) 185299..185712 (+) 91190406 V12G01-21538 (luxR) promoter; V12G01-14830 (aphA) promoter
REG0283 experimental V12G01_06711 (HP) protein QS regulatory pathway Vibrio alginolyticus 12G01 chromosome (AAPS01000024) 47707..48585 (-) 91188835 V12G01-21538 (luxR) promoter; V12G01-14830 (aphA) promoter
REG0284 experimental V12G01_16177 (MetR) protein QS regulatory pathway Vibrio alginolyticus 12G01 chromosome (AAPS01000023) 58420..59331 (-) 91188901 V12G01-21538 (luxR) promoter; V12G01-14830 (aphA) promoter
REG0285 experimental V12G01_02620 (SoxR) protein QS regulatory pathway Vibrio alginolyticus 12G01 chromosome (AAPS01000004) 181050..181484 (-) 91191052 V12G01-21538 (luxR) promoter; V12G01-14830 (aphA) promoter
REG0287 experimental V12G01_14830 (aphA) protein QS regulatory pathway Vibrio alginolyticus 12G01 chromosome (AAPS01000027) 42305..42880 (+) 91188695 V12G01-18672 (vqsA) promotor

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector