SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00337
Cognate effector	EFF01455
Related T6SS (Type)	T6SS00939 (Type i5)
Strain	Vibrio alginolyticus 12G01
Replicon	chromosome
Sequence	Protein sequence (300 a.a.); Nucleotide sequence (900 bp)
Description	-
Reference	[1] Salomon D, Klimko JA, Trudgian DC, et al. Type VI Secretion System Toxins Horizontally Shared between Marine Bacteria. PLoS Pathog. 2015 Aug 25;11(8):e1005128. doi: 10.1371/journal.ppat.1005128. eCollection 2015 Aug. PMID: 26305100

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	V12G01_RS35195
Coordinates (Strand)	503860..504759 (-)
NCBI ID	91191211
RefSeq	NZ_CH902590
Uniprot ID	UPI0000D52F3D
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Imm49	PF15575.8	2.5e-44	111..283

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-299	MVDNSKVKKVERVAHVELSDFEREAYREEIKESLDKRKRRISIKRENLVYLSDGISYQLRLYTWLPPLDDDERIGTLCLYESSLMRAFYHQLAIKPSEPQSIKLPDYPEVTWKGEGILKTGCLHPNRWLDAYFTSVIVRDKASMDVLANFPISLMRQSSTKAGELSYMLVDVIQSFHNRTSDYPDKLVAAMDAAVAQGDNWALEIAMGILETFAALTTNIGYDFEEVLVKNLQFQEKYHLRELGPDRIGIRTFVNFPLLGMACMWYDKGNRLSVETGWLPPYLVEGRWLEEVKAGKISR

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9977	-	-

Protein sequence: 300 a.a. .

                                    >IMU00337 NZ_CH902590:c504759-503860 [Vibrio alginolyticus 12G01]
MVDNSKVKKVERVAHVELSDFEREAYREEIKESLDKRKRRISIKRENLVYLSDGISYQLRLYTWLPPLDDDERIGTLCLY
ESSLMRAFYHQLAIKPSEPQSIKLPDYPEVTWKGEGILKTGCLHPNRWLDAYFTSVIVRDKASMDVLANFPISLMRQSST
KAGELSYMLVDVIQSFHNRTSDYPDKLVAAMDAAVAQGDNWALEIAMGILETFAALTTNIGYDFEEVLVKNLQFQEKYHL
RELGPDRIGIRTFVNFPLLGMACMWYDKGNRLSVETGWLPPYLVEGRWLEEVKAGKISR*

                                

Nucleotide sequence: 900 bp .

                                    >IMU00337 NZ_CH902590:c504759-503860 [Vibrio alginolyticus 12G01]
ATGGTTGATAACTCAAAGGTAAAAAAGGTTGAACGAGTAGCTCATGTTGAACTAAGTGATTTTGAAAGAGAAGCTTATCG
AGAAGAGATAAAAGAGAGCTTAGACAAAAGGAAAAGGCGCATATCTATAAAACGAGAAAACTTGGTTTACTTGAGCGATG
GTATTTCTTATCAGCTCCGTTTGTATACATGGTTACCTCCATTAGATGATGATGAGAGAATCGGCACGCTATGCCTATAT
GAATCATCATTGATGCGTGCTTTTTACCATCAACTTGCCATCAAACCAAGCGAGCCTCAAAGCATTAAATTACCTGATTA
TCCAGAAGTGACCTGGAAAGGTGAAGGTATTTTGAAAACTGGATGCTTACACCCTAATCGATGGTTAGATGCCTATTTTA
CCTCTGTCATCGTCCGTGACAAAGCTAGCATGGATGTGTTGGCGAACTTTCCGATTTCTTTGATGAGACAGAGCTCTACT
AAAGCGGGTGAATTGTCTTACATGTTAGTAGATGTCATCCAGTCGTTTCATAATCGCACATCGGACTATCCTGATAAACT
CGTCGCAGCAATGGACGCAGCGGTAGCCCAAGGGGATAACTGGGCGTTGGAAATTGCGATGGGGATCCTAGAAACCTTCG
CAGCGCTCACTACTAACATTGGTTATGATTTTGAGGAAGTGCTCGTTAAAAACCTACAGTTTCAAGAGAAATATCATCTT
CGAGAATTAGGACCCGACAGGATAGGCATCCGAACTTTTGTTAACTTCCCTCTTCTTGGCATGGCATGTATGTGGTATGA
CAAAGGTAACCGTTTATCTGTTGAAACTGGTTGGCTCCCTCCCTATCTTGTCGAAGGACGTTGGCTAGAAGAAGTAAAAG
CCGGGAAGATCTCTCGATAA

                                