Detailed information of effector


Summary

Effector ID EFF01455 experimental
Effector type Tde
Cognate immunity protein IMU00337 experimental
Related T6SS (Type) T6SS00939 experimental (Type i5)
Strain Vibrio alginolyticus 12G01
Replicon chromosome
Sequence Protein sequence (1462 a.a.); Nucleotide sequence (4386 bp)
Description V12G01_18287 possess predicted nuclease
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) V12G01_RS35200
Coordinates (Strand) 504752..509137 (-)
NCBI ID 491519899
RefSeq NZ_CH902590
Uniprot ID UPI0000D52E4E
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DUF6531 PF20148.1 6.9e-14 153..223
RHS PF03527.16 1.8e-13 1029..1062
RHS_repeat PF05593.16 2.2e-07 422..458
RHS_repeat PF05593.16 2e-05 886..913
RHS_repeat PF05593.16 6.4e-05 954..986
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-1461 MIPQFVIPITNRFGESYHFSGQLLTSGEPKTFVTKQEAIEFLESFGELDLRSVEDLFYAIGQFKPNIPDGELTSQHYNSTFANALVNGDLHVARIVPTGENYALSSAVTPPPTQNTVNAKPSKGKGKSNKKSGRSAASAKPQQQSSEKHEKAGDPVSLVTGEEILELYDIELQQGTTWQRTYRSSQSDVNHGMGFGWRHAFQFELREKTDEKDNVIAWEFVDDKADITEFEPVDIGATSYQIYAGASCYFLSSTTRIVTLANGHQYRFEQKDDRWLVKQVRNGTFSTFQLQYSRNHRLIEIAYNKRPVLECQYDKQGRLVELLNAKSQKVLTTYTYDEHDDLVSATNHLGLKEQYQYRGHLIVKRVRPTGFTHYFEWDGEGANANCIRNVGDAGIYDYRFHYQGNHSSYSDSLDHQWSFLHDEQGNLLEKISPTGKTWRWQYDELGRKAQAIYPDQSTVNYQYNQHGQLVSKFHSSGKEIQYGYDGLGKLVKTVSPDGDIEKAYYNSLGQRVWDTDQFGCVTEYDYDKHGQVVKKESEDGKKSQWWWDEQQRLIANEIDGTLLRYSYNEEDLVNGVAYPDGCIAQLAYDQFGRRTSVRYFNDEDKVGYSEEYSYDEHSRVAQIRTPAGITSYQWGSLAQQEAVIFPDGSHISYEYDQQRNLTKLVRSDGLEFEFLYDDEGQLSGTIGFDNLQSQFDYDDMGRIHRKQVADRTVLYRYDAAGYLKHVKAGNASGIVEHHFNYTLGGRLTFASNRHQTLQYQYSSFGHLTKRIQGQFEIAQEFNRVGQRVSQVLPDNTELNFSYDRYGRFSAIRFGNLDLPQIKFHYDEMGRVSQIETAHFNERIIYDGVGRLVEQHWPDREKKYVYNAQNRISSIIDNTAGATHYQYDALGQLKRVNASGEVTKFEYDSFGNPTLPSSEVFADRIEVHDGVRFKYDAQGNQTKREGQGHVQTRVFDALNQLIEVHTDDGISQYEYDALGRRTKKITQQGVTEFLWEGERLIGERSSCAFRWYLYQPETYTPVALLENDVIYWYQCDQVGKPERLIDCEGNVVWSASYDAHGFAHVHIEKVVNPLRFQGQYFDQETDLHYNLARYYDPKLGRFIQQDPISIAGGINHYQYAVNPIQWIDPTGFLCEEGLKRLGQMLAAYQAQSDVPQEVCDQILEAAKESSVGEDGVRSQVKIRKPKGKNHIRYEYDLDHIDCEKNEITFYRHINHSDGSRHEVQYVVGIEEFVNAHVFDSEKVGGEEGCTIFTEDLVLNKAEYKENSELNTSDYELTERNKEKIDECLKERQEAIDARAGEEGYNAQIGNINQQSAKIGELAADDFVRSKRPNAKLLHPKDIGTSISKPGDFDMVYEVEEPPPGEIIIVEAKGGSSPLGSRKIGNMAYQQGTTEYATEIVSLMDSKKYGTTEKAAANSINSAIQDGIPIRYVHTQAEISSSGKVSKFIAKEFDIDIGEFRNG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9881 - -


  Protein sequence: 1462 a.a.    .

>EFF01455 NZ_CH902590:c509137-504752 Type VI DNase and RNase Effector (Tde) [Vibrio alginolyticus 12G01]
MIPQFVIPITNRFGESYHFSGQLLTSGEPKTFVTKQEAIEFLESFGELDLRSVEDLFYAIGQFKPNIPDGELTSQHYNST
FANALVNGDLHVARIVPTGENYALSSAVTPPPTQNTVNAKPSKGKGKSNKKSGRSAASAKPQQQSSEKHEKAGDPVSLVT
GEEILELYDIELQQGTTWQRTYRSSQSDVNHGMGFGWRHAFQFELREKTDEKDNVIAWEFVDDKADITEFEPVDIGATSY
QIYAGASCYFLSSTTRIVTLANGHQYRFEQKDDRWLVKQVRNGTFSTFQLQYSRNHRLIEIAYNKRPVLECQYDKQGRLV
ELLNAKSQKVLTTYTYDEHDDLVSATNHLGLKEQYQYRGHLIVKRVRPTGFTHYFEWDGEGANANCIRNVGDAGIYDYRF
HYQGNHSSYSDSLDHQWSFLHDEQGNLLEKISPTGKTWRWQYDELGRKAQAIYPDQSTVNYQYNQHGQLVSKFHSSGKEI
QYGYDGLGKLVKTVSPDGDIEKAYYNSLGQRVWDTDQFGCVTEYDYDKHGQVVKKESEDGKKSQWWWDEQQRLIANEIDG
TLLRYSYNEEDLVNGVAYPDGCIAQLAYDQFGRRTSVRYFNDEDKVGYSEEYSYDEHSRVAQIRTPAGITSYQWGSLAQQ
EAVIFPDGSHISYEYDQQRNLTKLVRSDGLEFEFLYDDEGQLSGTIGFDNLQSQFDYDDMGRIHRKQVADRTVLYRYDAA
GYLKHVKAGNASGIVEHHFNYTLGGRLTFASNRHQTLQYQYSSFGHLTKRIQGQFEIAQEFNRVGQRVSQVLPDNTELNF
SYDRYGRFSAIRFGNLDLPQIKFHYDEMGRVSQIETAHFNERIIYDGVGRLVEQHWPDREKKYVYNAQNRISSIIDNTAG
ATHYQYDALGQLKRVNASGEVTKFEYDSFGNPTLPSSEVFADRIEVHDGVRFKYDAQGNQTKREGQGHVQTRVFDALNQL
IEVHTDDGISQYEYDALGRRTKKITQQGVTEFLWEGERLIGERSSCAFRWYLYQPETYTPVALLENDVIYWYQCDQVGKP
ERLIDCEGNVVWSASYDAHGFAHVHIEKVVNPLRFQGQYFDQETDLHYNLARYYDPKLGRFIQQDPISIAGGINHYQYAV
NPIQWIDPTGFLCEEGLKRLGQMLAAYQAQSDVPQEVCDQILEAAKESSVGEDGVRSQVKIRKPKGKNHIRYEYDLDHID
CEKNEITFYRHINHSDGSRHEVQYVVGIEEFVNAHVFDSEKVGGEEGCTIFTEDLVLNKAEYKENSELNTSDYELTERNK
EKIDECLKERQEAIDARAGEEGYNAQIGNINQQSAKIGELAADDFVRSKRPNAKLLHPKDIGTSISKPGDFDMVYEVEEP
PPGEIIIVEAKGGSSPLGSRKIGNMAYQQGTTEYATEIVSLMDSKKYGTTEKAAANSINSAIQDGIPIRYVHTQAEISSS
GKVSKFIAKEFDIDIGEFRNG*

  Nucleotide sequence: 4386 bp    .

>EFF01455 NZ_CH902590:c509137-504752 Type VI DNase and RNase Effector (Tde) [Vibrio alginolyticus 12G01]
ATGATCCCTCAATTCGTCATTCCTATCACGAATCGCTTTGGCGAAAGTTATCACTTCTCTGGTCAGTTACTTACTAGCGG
CGAACCTAAAACATTCGTGACCAAACAAGAGGCAATAGAGTTTCTTGAGAGCTTTGGTGAGCTTGACCTTCGCTCTGTCG
AGGACTTGTTCTATGCGATTGGTCAATTCAAGCCAAACATACCTGACGGTGAGCTTACTTCCCAACACTATAATTCAACG
TTTGCCAATGCTTTGGTGAACGGTGATTTGCATGTCGCTCGAATTGTACCTACCGGCGAAAACTACGCACTCTCGTCAGC
GGTTACGCCTCCACCGACTCAAAATACCGTCAATGCAAAACCATCGAAGGGAAAGGGGAAGTCGAATAAAAAGAGTGGCA
GAAGTGCCGCATCCGCCAAGCCTCAGCAACAAAGCAGCGAAAAACATGAGAAAGCCGGTGATCCTGTATCACTTGTGACG
GGTGAAGAAATTCTCGAACTCTATGATATCGAGCTACAACAAGGTACTACTTGGCAGCGTACTTACCGCTCTTCGCAGTC
GGATGTAAATCATGGAATGGGCTTTGGTTGGCGACATGCATTTCAGTTTGAATTACGCGAAAAGACGGACGAGAAAGACA
ATGTTATCGCTTGGGAGTTTGTTGACGATAAGGCCGATATCACAGAATTTGAGCCAGTAGATATTGGAGCAACAAGTTAT
CAAATATACGCTGGTGCGAGCTGTTACTTCCTGAGCTCAACAACTCGGATTGTAACGCTCGCTAATGGGCATCAATATCG
GTTTGAGCAAAAAGATGATCGCTGGCTTGTTAAACAAGTTAGAAATGGCACGTTTTCAACGTTTCAATTGCAGTATTCTC
GAAACCATCGATTGATCGAGATCGCATATAACAAGCGTCCGGTTCTTGAATGTCAATATGATAAGCAAGGACGCCTTGTA
GAACTGCTTAATGCAAAGTCCCAAAAAGTTCTGACAACTTATACCTATGATGAGCACGATGATTTGGTCAGTGCAACTAA
TCATTTGGGCTTGAAGGAACAGTATCAATACCGCGGCCATTTAATTGTTAAGCGTGTACGACCAACTGGCTTTACTCATT
ATTTTGAATGGGATGGCGAAGGTGCTAACGCGAACTGTATCAGGAACGTTGGGGACGCAGGAATTTATGACTATCGCTTC
CATTATCAGGGAAATCATTCTTCTTATTCTGACTCGTTAGATCATCAATGGTCTTTCTTGCATGACGAGCAGGGTAATTT
ACTCGAGAAAATAAGCCCCACAGGTAAAACTTGGCGATGGCAATATGATGAATTAGGACGAAAAGCACAAGCTATCTATC
CTGATCAGTCAACGGTCAATTATCAATATAATCAGCATGGTCAATTAGTCTCTAAGTTTCATTCCTCTGGAAAAGAAATT
CAATATGGTTATGACGGCTTAGGTAAGTTAGTTAAAACGGTTTCACCTGATGGGGATATCGAGAAGGCTTACTACAATAG
CCTCGGTCAAAGAGTTTGGGATACCGATCAGTTCGGCTGTGTCACGGAATATGATTACGATAAACACGGTCAAGTCGTCA
AAAAAGAGTCTGAAGATGGTAAGAAAAGTCAATGGTGGTGGGATGAACAACAAAGACTTATCGCGAATGAAATAGACGGT
ACATTACTTCGTTACAGCTACAACGAAGAAGATCTGGTGAATGGCGTTGCTTATCCCGATGGGTGTATTGCTCAACTCGC
TTATGATCAATTTGGCAGGCGTACATCAGTTCGTTATTTTAACGACGAAGACAAAGTCGGTTATAGCGAAGAATATTCTT
ACGATGAGCACAGCCGTGTTGCTCAGATTCGAACGCCAGCAGGCATTACCTCGTATCAATGGGGATCACTCGCGCAACAA
GAAGCAGTCATCTTTCCCGATGGCAGCCATATTTCTTACGAATACGATCAACAACGTAACCTGACTAAATTAGTTCGGAG
TGACGGCTTAGAGTTCGAATTCCTGTATGACGATGAAGGCCAGCTGTCAGGAACTATCGGGTTTGATAATCTACAAAGCC
AATTTGATTATGACGATATGGGGCGGATCCATCGTAAGCAAGTCGCCGATAGGACCGTGCTGTACCGCTATGACGCTGCG
GGTTATCTCAAGCACGTCAAAGCAGGAAATGCTAGCGGTATTGTAGAGCACCACTTTAATTATACGCTTGGTGGGCGACT
TACGTTCGCCTCCAATCGCCATCAAACATTGCAATACCAATACTCTTCATTCGGGCATTTAACAAAGCGCATTCAAGGTC
AGTTTGAGATAGCGCAGGAGTTTAACAGAGTTGGCCAACGCGTATCTCAGGTACTGCCAGATAATACGGAGCTGAACTTC
TCTTATGATCGATATGGGCGTTTTTCAGCGATTCGGTTTGGAAACCTAGATTTACCTCAAATCAAATTTCATTACGATGA
AATGGGACGAGTTTCCCAAATTGAAACGGCACATTTTAATGAGCGCATCATTTATGATGGTGTTGGTCGTTTGGTTGAAC
AACATTGGCCAGATAGAGAGAAAAAGTACGTTTACAATGCGCAAAACCGTATTTCATCAATCATTGATAATACGGCAGGA
GCAACGCACTATCAGTACGATGCCTTAGGGCAACTGAAACGAGTCAATGCTTCCGGAGAAGTGACTAAGTTTGAATACGA
TAGTTTTGGCAATCCTACTTTGCCATCTTCAGAGGTATTCGCTGATCGTATTGAAGTACACGATGGTGTCCGGTTCAAAT
ATGACGCGCAAGGAAACCAGACAAAACGTGAGGGGCAAGGACATGTTCAGACTCGCGTATTTGATGCGTTAAATCAGCTC
ATTGAAGTTCACACCGACGATGGCATCAGTCAATATGAATATGATGCATTAGGGCGCAGGACGAAGAAAATTACACAACA
AGGCGTTACGGAGTTCTTGTGGGAAGGGGAACGTTTAATTGGTGAACGCTCTTCATGCGCTTTCCGCTGGTACCTATATC
AACCAGAAACCTACACGCCAGTGGCACTGCTAGAAAATGACGTTATTTATTGGTACCAGTGCGATCAAGTTGGTAAACCA
GAGCGACTGATCGATTGCGAGGGGAATGTTGTATGGAGTGCCAGCTACGATGCTCATGGCTTTGCTCATGTACATATTGA
AAAAGTAGTAAATCCACTGCGTTTTCAAGGGCAGTACTTCGATCAAGAAACCGACTTACACTACAACCTCGCTCGGTATT
ACGATCCTAAGCTAGGACGCTTTATCCAACAAGATCCGATATCTATTGCTGGTGGCATTAACCATTACCAATATGCAGTC
AATCCTATCCAGTGGATTGATCCAACAGGTTTCCTTTGTGAAGAAGGGTTAAAACGCTTGGGTCAAATGTTGGCAGCGTA
TCAGGCGCAAAGCGATGTCCCCCAAGAGGTTTGCGATCAGATTTTAGAGGCAGCAAAAGAATCATCCGTCGGAGAAGATG
GCGTTCGCTCGCAAGTCAAAATCCGAAAACCCAAAGGTAAAAATCATATTCGATATGAATACGATTTAGACCATATTGAT
TGCGAGAAAAATGAAATTACTTTTTATCGTCATATCAATCATTCAGATGGCAGCAGGCACGAAGTTCAGTATGTTGTTGG
GATTGAGGAATTTGTAAATGCGCATGTTTTTGACTCAGAGAAAGTTGGCGGGGAAGAAGGTTGCACGATCTTTACTGAAG
ATCTTGTGCTGAATAAAGCAGAGTATAAAGAAAACTCTGAACTCAATACGAGTGATTATGAGCTAACAGAAAGAAACAAA
GAAAAAATTGACGAATGCTTAAAAGAGCGTCAAGAGGCAATCGACGCTCGAGCTGGCGAAGAGGGATATAACGCTCAAAT
TGGTAATATTAACCAACAGTCTGCAAAAATAGGTGAGTTGGCCGCGGATGACTTTGTCCGGAGTAAACGTCCTAATGCTA
AGCTGCTTCATCCCAAAGATATTGGAACCTCAATTTCGAAGCCGGGCGACTTTGATATGGTTTACGAGGTGGAAGAGCCG
CCACCAGGTGAAATTATAATTGTTGAGGCTAAAGGAGGGAGCAGCCCGCTGGGCAGCCGCAAGATTGGAAATATGGCATA
TCAGCAAGGGACAACTGAATATGCGACAGAAATAGTATCTTTGATGGACAGTAAGAAATATGGGACTACAGAAAAGGCAG
CTGCTAATAGCATTAACTCTGCTATTCAAGATGGTATACCAATACGTTATGTACATACGCAAGCAGAAATATCTTCTAGT
GGTAAGGTTTCCAAGTTCATAGCAAAAGAGTTTGATATAGATATAGGAGAGTTCAGAAATGGTTGA