SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01456
Effector type	Tme
Cognate immunity protein	IMU00338
Related T6SS (Type)	T6SS00939 (Type i5)
Strain	Vibrio alginolyticus 12G01
Replicon	chromosome
Sequence	Protein sequence (321 a.a.); Nucleotide sequence (963 bp)
Description	V12G01_03175 possess pore-forming colicin-like domains
Reference	[1] Salomon D, Klimko JA, Trudgian DC, et al. Type VI Secretion System Toxins Horizontally Shared between Marine Bacteria. PLoS Pathog. 2015 Aug 25;11(8):e1005128. doi: 10.1371/journal.ppat.1005128. eCollection 2015 Aug. PMID: 26305100

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	V12G01_RS27070
Coordinates (Strand)	494035..494997 (+)
NCBI ID	491516494
RefSeq	NZ_CH902589
Uniprot ID	A0A0P7EX22_VIBAL
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-320	MVLTHIMTLNKHISDPNRIRAGDIIWLSALPKSSSQLKTQSNNALPPKSIAKLGIYPASKPQMPSSPPHKVLPPNLLSYNQPPKSISNQVPPQHADMFWALAWLENNANMITIPGGTLMGATGNLMSKGNIKLIENINDLYANYKNGVISKGQYDGRRKKHLDLLKKNVGPMEKLLFGNKTTHQSIRIARSGGLPANAHIVKQAEKLNKIAQASKVGGHVLVGVGLTASCIQIANTQDQHEKNEIFVESITSTAVGMGTSALVGLFLVSNPIGWGTAIVLAAGSTAVGYASGKVAKSFYNSIESKPNLVASTGIESICSK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9185	-	-

Protein sequence: 321 a.a. .

                                    >EFF01456 NZ_CH902589:494035-494997 Type VI Membrane-disrupting Effector (Tme) [Vibrio alginolyticus 12G01]
MVLTHIMTLNKHISDPNRIRAGDIIWLSALPKSSSQLKTQSNNALPPKSIAKLGIYPASKPQMPSSPPHKVLPPNLLSYN
QPPKSISNQVPPQHADMFWALAWLENNANMITIPGGTLMGATGNLMSKGNIKLIENINDLYANYKNGVISKGQYDGRRKK
HLDLLKKNVGPMEKLLFGNKTTHQSIRIARSGGLPANAHIVKQAEKLNKIAQASKVGGHVLVGVGLTASCIQIANTQDQH
EKNEIFVESITSTAVGMGTSALVGLFLVSNPIGWGTAIVLAAGSTAVGYASGKVAKSFYNSIESKPNLVASTGIESICSK
*

                                

Nucleotide sequence: 963 bp .

                                    >EFF01456 NZ_CH902589:494035-494997 Type VI Membrane-disrupting Effector (Tme) [Vibrio alginolyticus 12G01]
ATGGTACTGACTCATATAATGACATTAAACAAGCATATTTCAGATCCTAACCGCATTAGAGCAGGCGATATCATTTGGTT
ATCAGCTCTACCCAAATCAAGCTCTCAACTTAAAACTCAAAGTAACAATGCGTTACCTCCTAAAAGTATCGCGAAGCTAG
GGATCTACCCAGCGTCTAAACCTCAAATGCCCAGCTCTCCTCCCCATAAAGTTTTGCCTCCCAATCTTCTCAGCTACAAT
CAACCTCCAAAATCAATCTCAAATCAAGTTCCACCGCAACATGCTGACATGTTTTGGGCGTTGGCATGGCTAGAAAACAA
CGCCAATATGATTACTATTCCAGGAGGTACCTTGATGGGTGCTACAGGGAACTTGATGAGTAAAGGAAATATTAAGCTCA
TTGAGAACATCAACGACCTTTACGCAAACTATAAAAACGGAGTAATAAGTAAAGGACAATATGATGGTCGACGTAAAAAA
CATTTAGACCTACTAAAGAAAAATGTTGGCCCTATGGAAAAGCTGTTATTTGGCAACAAAACGACTCACCAGTCTATTCG
CATAGCTCGAAGTGGAGGGTTACCTGCGAATGCTCATATAGTTAAACAAGCCGAAAAACTGAATAAAATAGCTCAAGCAT
CTAAAGTGGGCGGACATGTTTTAGTCGGTGTTGGTCTGACGGCTTCCTGCATTCAGATTGCGAATACACAAGACCAACAT
GAGAAGAATGAAATTTTTGTTGAGTCAATAACTAGCACAGCTGTAGGAATGGGTACCAGCGCGTTAGTCGGTCTATTTTT
AGTATCAAACCCTATTGGCTGGGGGACTGCCATTGTACTCGCTGCCGGTTCAACGGCTGTAGGTTATGCATCCGGTAAAG
TGGCAAAAAGCTTTTATAATTCAATTGAAAGCAAGCCAAACTTAGTCGCTAGTACTGGAATAGAGTCTATATGCAGCAAG
TAA

                                