Detailed information of regulator
Summary
| Regulator ID | REG0278  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Transcription regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00939 (Type i5) |
| Strain | Vibrio alginolyticus 12G01 |
| Replicon | chromosome |
| Sequence | Protein sequence (136 a.a.); Nucleotide sequence (408 bp) |
| Description | H-NS is a repressor of VaT6SS1 activity |
| Reference |
External database links
| Locus tag (Gene) | V12G01_20201 (H-NS) |
| Coordinates (Strand) | 63783..64190 (+) |
| NCBI ID | 91189474 |
| GenBank | AAPS01000015 |
| Uniprot ID | UPI0000D54A10 |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| VaT6SS1 transcripts | ↑ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Histone_HNS | PF00816.23 | 4.7e-19 | 35..129 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-135 | MSELTKTLLNIRSLRAFSRELTLEQLEEALDKLTTVVDERREAEEAERAAQAEQEAKLAEIAEKIAQDGIDVEALITALSGETKTKGKAKRAPRPAKYKYTDSNGNEKTWTGQGRTPSAIQEQLDAGKSLDEFLI |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9953 | - | - |
Protein sequence of regulator: 136 a.a. .
>REG0278 AAPS01000015:63783-64190 [Vibrio alginolyticus 12G01]
MSELTKTLLNIRSLRAFSRELTLEQLEEALDKLTTVVDERREAEEAERAAQAEQEAKLAEIAEKIAQDGIDVEALITALS
GETKTKGKAKRAPRPAKYKYTDSNGNEKTWTGQGRTPSAIQEQLDAGKSLDEFLI*
MSELTKTLLNIRSLRAFSRELTLEQLEEALDKLTTVVDERREAEEAERAAQAEQEAKLAEIAEKIAQDGIDVEALITALS
GETKTKGKAKRAPRPAKYKYTDSNGNEKTWTGQGRTPSAIQEQLDAGKSLDEFLI*
Nucleotide sequence of regulator: 408 bp .
>REG0278 AAPS01000015:63783-64190 [Vibrio alginolyticus 12G01]
ATGTCAGAGCTGACTAAAACCCTTCTAAATATCCGTAGCCTTCGTGCATTTTCTCGTGAGCTGACTCTAGAACAATTGGA
AGAAGCTCTAGATAAACTCACTACTGTCGTAGATGAGCGTCGTGAAGCAGAAGAAGCAGAACGCGCAGCACAAGCAGAGC
AAGAAGCTAAGCTTGCAGAGATTGCTGAGAAAATCGCTCAAGACGGTATTGACGTAGAAGCACTTATTACTGCGCTATCT
GGCGAAACTAAGACGAAAGGCAAAGCGAAGCGCGCACCTCGCCCTGCTAAATACAAATACACAGATTCAAACGGTAATGA
GAAAACTTGGACTGGCCAAGGTCGTACACCTTCGGCGATTCAAGAGCAACTTGATGCAGGTAAATCTCTGGATGAGTTCC
TAATCTAG
ATGTCAGAGCTGACTAAAACCCTTCTAAATATCCGTAGCCTTCGTGCATTTTCTCGTGAGCTGACTCTAGAACAATTGGA
AGAAGCTCTAGATAAACTCACTACTGTCGTAGATGAGCGTCGTGAAGCAGAAGAAGCAGAACGCGCAGCACAAGCAGAGC
AAGAAGCTAAGCTTGCAGAGATTGCTGAGAAAATCGCTCAAGACGGTATTGACGTAGAAGCACTTATTACTGCGCTATCT
GGCGAAACTAAGACGAAAGGCAAAGCGAAGCGCGCACCTCGCCCTGCTAAATACAAATACACAGATTCAAACGGTAATGA
GAAAACTTGGACTGGCCAAGGTCGTACACCTTCGGCGATTCAAGAGCAACTTGATGCAGGTAAATCTCTGGATGAGTTCC
TAATCTAG