SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015465
Component protein type	TagE
T6SS ID (Type)	T6SS00939 (Type i5)
Strain	Vibrio alginolyticus 12G01
Replicon	chromosome
Sequence	Protein sequence (717 a.a.); Nucleotide sequence (2151 bp)
Reference	[1] Salomon D, Kinch LN, Trudgian DC, et al. Marker for type VI secretion system effectors. Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9271-6. doi: 10.1073/pnas.1406110111. Epub 2014 Jun 9. PMID: 24927539 [2] Salomon D, Klimko JA, Trudgian DC, et al. Type VI Secretion System Toxins Horizontally Shared between Marine Bacteria. PLoS Pathog. 2015 Aug 25;11(8):e1005128. doi: 10.1371/journal.ppat.1005128. eCollection 2015 Aug. PMID: 26305100

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	V12G01_RS31160
Coordinate (Strand)	1440993..1443143 (-)
NCBI ID	491518297
RefSeq	NZ_CH902589
Uniprot ID	UPI0000D5401D
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Pkinase	PF00069.27	3.2e-39	93..328

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-716	MEDSNKNNSINNEESGDKTKIVKTQKRDNAVKSTKLTNNETSVKNKNETNTKLSQKENTPLVNKKKNNKDDSLNNNKDVINESLIGATVKNRYEIESVIGHGGLCDVYLAKDRILESSGSETPYVALKILQKEFASQPETARMLIREAQQTQRLSHPNIIRVFDFGVDGEIYFLVMEYINGETLESLIQRSRPHGLKFNATLSILNQVLDALSYAHSLGVVHADLKPANVILTSEGNVKLLDFGVSKTHQLKQDQYAAKRKSENVETLGYTPNYASLNLLSGKEPNLSDDMFALFCIAYELLSCKHPYARTPVNEALKKKIKVSKPTNMPASKWQIFSSVFSTGIIPNELTPEQLKRKLNMRYWPIASGAAASILLVSGIVFGYQQQQETIANLETVISKQNDQIAGQEWQLDTPAEKSLALIDSEKSLHPVVKAGLLRLHKTYILSEFESEIDQVLNSDANNYPNYDEIESILDKAKTYYPDSHKIEVLALDIQSSKHSTLLSIARQINSQLEKSRYSKEDGSKSIYELKDELNQIHQGYPFTPSSLSSDVFGQHLTEALQKKDAAALVALIKVGNTFFSNVEEHQENLQISNAMKDAVLEMKLYETALDSNSPLPFPEDAARILFQKEFENFYSRLEKARTTTHLDNLVKDVDLFSKNFPIGFQDVNDLRFKTADKYLRFSDILLNKRRTSSARRAMKKANELMKQIEQDSKQS

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9515	-	-

Protein sequence: 717 a.a.

						>T6CP015465 NZ_CH902589:c1443143-1440993 [Vibrio alginolyticus 12G01] [TagE]
MEDSNKNNSINNEESGDKTKIVKTQKRDNAVKSTKLTNNETSVKNKNETNTKLSQKENTPLVNKKKNNKDDSLNNNKDVI
NESLIGATVKNRYEIESVIGHGGLCDVYLAKDRILESSGSETPYVALKILQKEFASQPETARMLIREAQQTQRLSHPNII
RVFDFGVDGEIYFLVMEYINGETLESLIQRSRPHGLKFNATLSILNQVLDALSYAHSLGVVHADLKPANVILTSEGNVKL
LDFGVSKTHQLKQDQYAAKRKSENVETLGYTPNYASLNLLSGKEPNLSDDMFALFCIAYELLSCKHPYARTPVNEALKKK
IKVSKPTNMPASKWQIFSSVFSTGIIPNELTPEQLKRKLNMRYWPIASGAAASILLVSGIVFGYQQQQETIANLETVISK
QNDQIAGQEWQLDTPAEKSLALIDSEKSLHPVVKAGLLRLHKTYILSEFESEIDQVLNSDANNYPNYDEIESILDKAKTY
YPDSHKIEVLALDIQSSKHSTLLSIARQINSQLEKSRYSKEDGSKSIYELKDELNQIHQGYPFTPSSLSSDVFGQHLTEA
LQKKDAAALVALIKVGNTFFSNVEEHQENLQISNAMKDAVLEMKLYETALDSNSPLPFPEDAARILFQKEFENFYSRLEK
ARTTTHLDNLVKDVDLFSKNFPIGFQDVNDLRFKTADKYLRFSDILLNKRRTSSARRAMKKANELMKQIEQDSKQS*					

Nucleotide sequence: 2151 bp

						>T6CP015465 NZ_CH902589:c1443143-1440993 [Vibrio alginolyticus 12G01] [TagE]
ATGGAAGACAGTAATAAAAACAATTCTATAAATAACGAAGAAAGCGGAGATAAAACTAAAATTGTAAAGACTCAAAAACG
AGACAATGCAGTTAAATCAACCAAACTCACTAATAATGAAACCTCCGTTAAAAATAAAAATGAAACAAATACAAAACTGT
CTCAAAAAGAAAACACTCCGTTAGTTAATAAGAAAAAAAACAATAAAGATGACTCATTAAATAATAACAAAGATGTTATT
AACGAAAGCTTAATAGGAGCGACCGTTAAAAATCGGTATGAAATTGAGTCGGTTATTGGACATGGTGGGCTTTGCGATGT
TTACTTAGCTAAAGATAGGATTCTTGAATCTTCAGGTTCAGAAACACCCTACGTTGCGTTAAAAATATTACAGAAAGAGT
TTGCTTCTCAACCAGAGACTGCACGCATGTTAATACGTGAAGCTCAACAAACTCAAAGGTTAAGCCACCCTAATATCATT
AGGGTTTTTGATTTTGGTGTTGATGGCGAAATATACTTTTTAGTTATGGAGTATATTAACGGAGAAACATTAGAGAGTTT
AATACAGCGTTCTAGGCCCCATGGGCTGAAGTTTAATGCAACTTTATCAATCCTAAATCAGGTACTCGATGCACTCTCAT
ATGCTCACTCTCTTGGTGTAGTGCACGCCGACCTAAAGCCCGCTAATGTGATTTTAACGTCTGAGGGTAACGTTAAGTTG
CTTGATTTTGGTGTATCTAAAACTCATCAATTAAAGCAGGATCAATATGCGGCTAAACGTAAATCCGAAAATGTTGAAAC
GCTTGGTTATACTCCCAACTATGCATCACTCAACCTGTTATCCGGTAAGGAACCGAACTTAAGCGACGATATGTTTGCTC
TATTTTGTATTGCTTACGAGCTATTGAGTTGCAAACACCCTTACGCAAGAACTCCAGTTAATGAAGCTCTCAAAAAGAAG
ATCAAGGTCTCTAAACCAACTAACATGCCTGCATCTAAGTGGCAAATATTTTCAAGCGTATTTTCAACTGGCATCATTCC
GAACGAGCTGACCCCTGAGCAGTTGAAACGCAAATTGAATATGCGCTATTGGCCTATCGCTAGCGGCGCGGCAGCGAGTA
TATTATTAGTTAGCGGCATTGTATTTGGTTATCAGCAGCAACAAGAAACCATCGCCAATTTGGAAACCGTTATTAGCAAG
CAAAATGATCAAATTGCCGGACAAGAGTGGCAGCTTGATACGCCAGCAGAAAAAAGTTTGGCACTGATTGATAGTGAAAA
AAGCTTACATCCAGTCGTTAAGGCTGGCTTATTACGCTTACACAAGACCTATATTCTCAGCGAGTTTGAATCTGAAATTG
ATCAGGTTTTGAATTCTGATGCCAACAACTACCCGAATTATGATGAAATTGAGTCCATACTTGATAAAGCAAAAACCTAC
TACCCTGACTCACATAAAATAGAAGTTCTTGCCTTAGATATTCAGTCAAGCAAGCACTCCACTTTACTTTCGATAGCAAG
GCAAATCAACAGTCAACTTGAAAAGTCTCGGTACAGTAAAGAAGACGGCAGTAAATCCATCTATGAGCTCAAGGATGAGT
TAAATCAGATTCATCAAGGTTATCCTTTCACGCCAAGTTCGTTATCTAGCGATGTTTTCGGTCAGCACCTCACTGAAGCA
CTCCAAAAGAAAGACGCAGCTGCACTTGTTGCTCTTATTAAGGTTGGGAACACCTTCTTTTCAAACGTAGAAGAGCATCA
AGAGAACTTACAGATAAGTAATGCGATGAAAGATGCAGTGCTGGAAATGAAATTATATGAGACAGCGCTAGATTCCAACA
GCCCTCTACCGTTTCCAGAAGATGCCGCTAGAATCCTGTTCCAAAAAGAGTTTGAAAACTTTTATTCTCGCCTAGAAAAG
GCAAGGACGACAACTCATCTCGATAACTTGGTGAAGGATGTGGATCTGTTCTCGAAAAACTTTCCTATTGGTTTTCAGGA
TGTTAACGATTTAAGATTCAAAACTGCTGATAAATACTTACGCTTCTCCGATATATTACTTAATAAGCGAAGGACTTCAA
GCGCAAGACGCGCTATGAAGAAAGCCAACGAGCTTATGAAGCAAATAGAACAGGACTCTAAGCAAAGCTAG					