Detailed information of component protein


Summary

Component ID T6CP015469
Component protein type TssL
T6SS ID (Type) T6SS00939 experimental (Type i5)
Strain Vibrio alginolyticus 12G01
Replicon chromosome
Sequence Protein sequence (431 a.a.); Nucleotide sequence (1293 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) V12G01_RS31180
Coordinate (Strand) 1446475..1447767 (+)
NCBI ID 491518308
RefSeq NZ_CH902589
Uniprot ID A0A0H0YGE8_VIBAL
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 2.1e-55 51..252
OmpA PF00691.22 1.4e-13 322..420
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-229 MEQTIVKPTPGGRAPASKPQSEKSVDNTVVISKSPELVNNDSVVAYGNNSLLAEANGLLSIIGQIRSTATHSDPLFLKETLAQKLRDYENRLRQHDVDLDTIDTARYCLCCSLDEVVLNTNWGGNSFWSHDSLLSSFYASSQGGEAFFKHLDKCMAKPDVNLDLLELMYVCLSLGFLGQYRLEKNGLESHRKLRRQVIATLKTYGRGVPQELSNSIDQHILNGSKASDR
Transmembrane helix 230-252 APLWVVCSVTAALLVCIYMYFSY
Inside 253-430 ELNKASNRTFSQLVNLIQPQQLNQDATSESKSAPIAEKISMYLATEIGKGLINVEPLQDRVRISLKSQDLFESGSADVVAYIQPVISKLARTLESTQGKIIVTGHTDDRPIFTSKYPSNWHLSLARATAMSEQLISNSALEGRVIPEGLGDARPLVENSSDENRAINRRIEIDLLVAN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9447 - -


  Protein sequence: 431 a.a.    

>T6CP015469 NZ_CH902589:1446475-1447767 [Vibrio alginolyticus 12G01] [TssL]
MEQTIVKPTPGGRAPASKPQSEKSVDNTVVISKSPELVNNDSVVAYGNNSLLAEANGLLSIIGQIRSTATHSDPLFLKET
LAQKLRDYENRLRQHDVDLDTIDTARYCLCCSLDEVVLNTNWGGNSFWSHDSLLSSFYASSQGGEAFFKHLDKCMAKPDV
NLDLLELMYVCLSLGFLGQYRLEKNGLESHRKLRRQVIATLKTYGRGVPQELSNSIDQHILNGSKASDRAPLWVVCSVTA
ALLVCIYMYFSYELNKASNRTFSQLVNLIQPQQLNQDATSESKSAPIAEKISMYLATEIGKGLINVEPLQDRVRISLKSQ
DLFESGSADVVAYIQPVISKLARTLESTQGKIIVTGHTDDRPIFTSKYPSNWHLSLARATAMSEQLISNSALEGRVIPEG
LGDARPLVENSSDENRAINRRIEIDLLVAN*

  Nucleotide sequence: 1293 bp    

>T6CP015469 NZ_CH902589:1446475-1447767 [Vibrio alginolyticus 12G01] [TssL]
ATGGAACAAACGATAGTAAAACCAACACCGGGTGGAAGAGCACCCGCATCGAAACCACAATCAGAAAAGTCTGTGGATAA
TACCGTAGTTATCTCAAAAAGTCCTGAATTGGTGAACAATGATTCAGTGGTCGCTTATGGGAATAACTCTCTACTAGCAG
AGGCGAATGGCCTGTTATCTATTATCGGTCAAATTCGCTCGACAGCGACTCATTCCGATCCGTTGTTTCTAAAAGAGACA
TTGGCACAAAAGCTTAGAGATTATGAGAACCGATTACGTCAGCATGACGTTGATTTGGATACCATTGATACAGCTCGTTA
TTGTTTATGCTGTTCACTAGATGAAGTTGTCCTTAACACCAATTGGGGTGGGAATTCATTCTGGAGTCACGATTCATTAC
TTAGCTCCTTCTATGCTAGCAGTCAAGGTGGGGAAGCGTTCTTTAAGCATCTTGATAAGTGCATGGCGAAACCAGATGTG
AACTTAGATTTGCTTGAACTAATGTATGTGTGCTTATCTCTCGGGTTTCTGGGTCAATACCGTTTAGAAAAAAATGGACT
GGAATCGCACCGGAAATTGAGAAGACAGGTTATTGCTACTCTTAAGACTTATGGGAGAGGAGTCCCTCAGGAACTAAGCA
ATAGTATCGACCAACACATTTTGAATGGCTCAAAGGCTTCTGATAGAGCTCCTCTTTGGGTTGTATGTTCTGTAACGGCG
GCACTATTGGTCTGCATATATATGTATTTTAGTTATGAGCTAAATAAGGCATCCAATCGCACCTTTTCTCAATTAGTCAA
TTTGATTCAACCGCAACAACTTAACCAAGATGCAACGTCAGAATCAAAATCAGCGCCAATTGCAGAAAAGATTTCAATGT
ATCTAGCAACTGAGATCGGTAAGGGGCTAATCAATGTTGAGCCGCTGCAAGACAGGGTAAGAATTTCGTTAAAGTCTCAA
GATCTATTTGAATCAGGTAGTGCTGATGTTGTCGCTTACATCCAACCCGTTATTTCTAAGCTTGCGAGAACATTGGAGTC
GACCCAAGGCAAGATCATTGTTACTGGTCACACCGACGATAGGCCTATTTTCACGAGTAAATATCCCTCGAACTGGCACC
TATCATTAGCTCGAGCCACTGCTATGTCTGAACAACTGATCTCGAATAGTGCATTAGAAGGGCGAGTGATACCCGAGGGA
TTGGGGGATGCAAGACCATTAGTTGAAAACAGTAGTGATGAAAACCGAGCTATTAACCGCCGGATTGAAATCGACCTTCT
TGTCGCGAATTAA