SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015473
Component protein type	TssA
T6SS ID (Type)	T6SS00939 (Type i5)
Strain	Vibrio alginolyticus 12G01
Replicon	chromosome
Sequence	Protein sequence (372 a.a.); Nucleotide sequence (1116 bp)
Reference	[1] Salomon D, Kinch LN, Trudgian DC, et al. Marker for type VI secretion system effectors. Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9271-6. doi: 10.1073/pnas.1406110111. Epub 2014 Jun 9. PMID: 24927539 [2] Salomon D, Klimko JA, Trudgian DC, et al. Type VI Secretion System Toxins Horizontally Shared between Marine Bacteria. PLoS Pathog. 2015 Aug 25;11(8):e1005128. doi: 10.1371/journal.ppat.1005128. eCollection 2015 Aug. PMID: 26305100

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	V12G01_RS31200
Coordinate (Strand)	1452776..1453891 (+)
NCBI ID	491518317
RefSeq	NZ_CH902589
Uniprot ID	UPI0000D5406C
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	2.5e-32	13..142

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-371	MSNEMFDWYEQALAPISQENPTGIDPREDVSPQSAYYRLKDQRMAARNAERNALIEEESIYTHSNLWRVFIEDVPEILTNQSKDLEFVAWLIEALTRLYGFRGMGVGYKLATSLIENFWDDLYPMPDEDGIETRISAIIGLNGIDSEGTLIFPIACIPLTDLGVEQAYAYWEYQQAIELERLDEDKRRNRVELGAVELSEIITTVKSTSDTFYQELITDLEFATESFNEFSQRLDEVVGDVTPSSYISKRLESIHSALKHLLGDRFNQPQAVFEELEDLDESSSEQPLSSQSCDALLATNMKSREQAIRQLQQVADFFRESEPHSPVSYSIEQIIRWCGMPLPELLAELISDGDAKKSYFRLVGIAEQNEN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9876	-	-

Protein sequence: 372 a.a.

						>T6CP015473 NZ_CH902589:1452776-1453891 [Vibrio alginolyticus 12G01] [TssA]
MSNEMFDWYEQALAPISQENPTGIDPREDVSPQSAYYRLKDQRMAARNAERNALIEEESIYTHSNLWRVFIEDVPEILTN
QSKDLEFVAWLIEALTRLYGFRGMGVGYKLATSLIENFWDDLYPMPDEDGIETRISAIIGLNGIDSEGTLIFPIACIPLT
DLGVEQAYAYWEYQQAIELERLDEDKRRNRVELGAVELSEIITTVKSTSDTFYQELITDLEFATESFNEFSQRLDEVVGD
VTPSSYISKRLESIHSALKHLLGDRFNQPQAVFEELEDLDESSSEQPLSSQSCDALLATNMKSREQAIRQLQQVADFFRE
SEPHSPVSYSIEQIIRWCGMPLPELLAELISDGDAKKSYFRLVGIAEQNEN*					

Nucleotide sequence: 1116 bp

						>T6CP015473 NZ_CH902589:1452776-1453891 [Vibrio alginolyticus 12G01] [TssA]
ATGAGTAATGAAATGTTTGATTGGTATGAACAAGCGTTGGCTCCTATATCTCAAGAAAATCCTACAGGTATTGATCCTAG
AGAGGATGTATCACCACAGTCTGCTTACTATCGGTTGAAAGATCAGCGTATGGCCGCCCGCAACGCTGAACGTAATGCTC
TTATAGAAGAAGAAAGTATCTATACCCACAGTAATTTATGGCGAGTATTTATTGAAGATGTCCCAGAAATACTCACGAAT
CAGTCTAAAGATTTGGAATTTGTTGCCTGGCTTATAGAAGCCCTAACTCGATTGTATGGTTTTCGTGGTATGGGCGTTGG
CTACAAACTCGCAACCAGTTTAATTGAGAACTTTTGGGATGATCTGTACCCAATGCCTGATGAAGATGGTATTGAGACAA
GAATATCGGCGATTATTGGCCTCAATGGTATCGATAGTGAAGGAACACTTATTTTTCCAATTGCTTGTATACCATTGACT
GACTTAGGCGTGGAGCAAGCTTATGCCTATTGGGAATATCAGCAAGCTATAGAGCTTGAGCGCTTAGACGAAGATAAGCG
CCGAAATCGAGTCGAGCTTGGGGCTGTTGAGTTATCTGAAATTATAACTACGGTTAAGTCGACTTCTGATACGTTTTATC
AAGAATTAATCACGGATTTGGAGTTCGCTACAGAATCATTTAATGAATTCAGTCAGCGATTAGACGAAGTGGTCGGTGAT
GTGACTCCTAGCTCTTATATATCAAAAAGACTTGAATCAATACACTCGGCATTAAAGCACCTTCTAGGAGATCGTTTCAA
TCAACCACAAGCCGTGTTTGAAGAGCTAGAGGATCTTGATGAATCTTCTTCTGAACAACCGTTAAGTTCACAAAGTTGTG
ATGCATTATTAGCGACAAACATGAAATCACGAGAGCAGGCAATTAGACAATTGCAGCAAGTAGCTGATTTCTTCCGAGAG
AGTGAACCTCACTCACCAGTTTCTTACTCCATCGAACAGATTATTCGTTGGTGTGGGATGCCTCTGCCGGAGTTACTGGC
GGAACTTATCTCCGACGGGGATGCAAAGAAAAGCTACTTCCGTTTGGTTGGCATAGCAGAACAAAATGAAAATTAA					